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June 28th 2015
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June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015
Join us at the Metabolomics Conference tutorial
June 28th 2015

MetaCyc Enzyme: 2-methylcitrate dehydratase

Gene: prpD Accession Number: G-10383 (MetaCyc)

Species: Salmonella enterica enterica serovar Typhimurium

The prpD gene from Salmonella enterica enterica serovar Typhimurium was cloned and overexpressed in Escherichia coli, and the recombinant protein was purified and characterized. 2-methylcitrate dehydratase activity was demonstrated in vitro [Horswill01].

The authors reported that purified PrpD enzyme did not contain an iron-sulfur center, and displayed no requirements for metal cations. This contrasts with sequence analysis which predicts that the protein sould contain one 2Fe-2S cluster.

Gene Citations: [Horswill97]

Molecular Weight of Polypeptide: 53.787 kD (from nucleotide sequence), 54.0 kD (experimental) [Horswill01 ]

Unification Links: Entrez-gene:1251889 , Pride:P74840 , Protein Model Portal:P74840 , SMR:P74840 , String:99287.STM0370 , UniProt:P74840

Relationship Links: Entrez-Nucleotide:RELATED-TO:U51879 , InterPro:IN-FAMILY:IPR005656 , InterPro:IN-FAMILY:IPR012705 , Panther:IN-FAMILY:PTHR16943 , Pfam:IN-FAMILY:PF03972

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 16-Jan-2001 by Pellegrini-Toole A , Marine Biological Laboratory
Revised 12-Dec-2007 by Caspi R , SRI International

Enzymatic reaction of: 2-methylcitrate dehydratase

Synonyms: 2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase

EC Number:

(2S,3S)-2-methylcitrate <=> cis-2-methylaconitate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for (2S,3S)-2-methylcitrate: cis-aconitate [Horswill01 ]

In Pathways: 2-methylcitrate cycle I

The purified enzyme had a specific activity of 2.8 μmol/min/mg protein. However, this value may be an underestimate of the dehydration rate of the substrate if the enzyme uses as substrate only one of the four 2-methylcitrate stereoisomers thatwere present in the mixture, or if any of the stereoisomers inhibited enzyme activity [Horswill01].


Horswill01: Horswill AR, Escalante-Semerena JC (2001). "In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate." Biochemistry 40(15);4703-13. PMID: 11294638

Horswill97: Horswill AR, Escalante-Semerena JC (1997). "Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon." J Bacteriol 179(3);928-40. PMID: 9006051

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Jun 30, 2015, biocyc14.