MetaCyc Enzyme: 2-methylcitrate dehydratase

Gene: prpD Accession Number: G-10383 (MetaCyc)

Species: Salmonella enterica enterica serovar Typhimurium

The prpD gene from Salmonella enterica enterica serovar Typhimurium was cloned and overexpressed in Escherichia coli, and the recombinant protein was purified and characterized. 2-methylcitrate dehydratase activity was demonstrated in vitro [Horswill01].

The authors reported that purified PrpD enzyme did not contain an iron-sulfur center, and displayed no requirements for metal cations. This contrasts with sequence analysis which predicts that the protein sould contain one 2Fe-2S cluster.

Gene Citations: [Horswill97]

Molecular Weight of Polypeptide: 53.787 kD (from nucleotide sequence), 54.0 kD (experimental) [Horswill01 ]

Unification Links: Entrez-gene:1251889 , Pride:P74840 , Protein Model Portal:P74840 , SMR:P74840 , String:99287.STM0370 , UniProt:P74840

Relationship Links: Entrez-Nucleotide:RELATED-TO:U51879 , InterPro:IN-FAMILY:IPR005656 , InterPro:IN-FAMILY:IPR012705 , Panther:IN-FAMILY:PTHR16943 , Pfam:IN-FAMILY:PF03972

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 16-Jan-2001 by Pellegrini-Toole A , Marine Biological Laboratory
Revised 12-Dec-2007 by Caspi R , SRI International

Enzymatic reaction of: 2-methylcitrate dehydratase

Synonyms: 2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase

EC Number:

(2S,3S)-2-methylcitrate <=> cis-2-methylaconitate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for (2S,3S)-2-methylcitrate: cis-aconitate [Horswill01 ]

In Pathways: 2-methylcitrate cycle I

The purified enzyme had a specific activity of 2.8 μmol/min/mg protein. However, this value may be an underestimate of the dehydration rate of the substrate if the enzyme uses as substrate only one of the four 2-methylcitrate stereoisomers thatwere present in the mixture, or if any of the stereoisomers inhibited enzyme activity [Horswill01].


Horswill01: Horswill AR, Escalante-Semerena JC (2001). "In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate." Biochemistry 40(15);4703-13. PMID: 11294638

Horswill97: Horswill AR, Escalante-Semerena JC (1997). "Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon." J Bacteriol 179(3);928-40. PMID: 9006051

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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