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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: mannosyltransferase

Gene: ALG9 Accession Number: G-5582 (MetaCyc)

Synonyms: Alg9p

Species: Saccharomyces cerevisiae

Summary:
Sequence analysis predicted that ALG9 encodes a protein with a cleavable signal sequence at the N-terminus. It appeared to be a hydrophobic protein with a hydrophilic C-terminal domain, and 4 potential N-linked glycosylation sites. An endoplasmic reticulum location was also suggested by these analyses [Burda96].
ALG9 mutants accumulate (mannosyl)6-(N-acetylglucosaminyl)2-diphosphodolichol [Burda96, Cipollo00].

Gene Citations: [Frank05a]

Locations: endoplasmic reticulum

Map Position: [235,995 <- 237,662]

Molecular Weight of Polypeptide: 63.8 kD (from nucleotide sequence)

Unification Links: DIP:DIP-4288N , Entrez-gene:855502 , Mint:MINT-507147 , ModBase:P53868 , Protein Model Portal:P53868 , String:4932.YNL219C , Swiss-Model:P53868 , UniProt:P53868

Relationship Links: CAZy:IN-FAMILY:GT22 , InterPro:IN-FAMILY:IPR005599 , Panther:IN-FAMILY:PTHR22760 , Pfam:IN-FAMILY:PF03901

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005783 - endoplasmic reticulum [Burda96]

Credits:
Revised 25-Sep-2006 by Fulcher CA , SRI International


Enzymatic reaction of: (mannosyl)8-(N-acetylglucosaminyl)2-diphosphodolichol:dolichyl β-D-mannosyl phosphate mannosyl transferase (mannosyltransferase)

EC Number: 2.4.1.261

a (mannosyl)8-(N-acetylglucosaminyl)2-diphosphodolichol + a dolichyl β-D-mannosyl phosphate <=> a (mannosyl)9-(N-acetylglucosaminyl)2-diphosphodolichol + a dolichyl phosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: dolichyl-diphosphooligosaccharide biosynthesis

Summary:
The Alg9 gene product is involved in the addition of the seventh and ninth mannose to the lipid-linked oligosaccharide chain [Frank05a].


Enzymatic reaction of: (mannosyl)6-(N-acetylglucosaminyl)2-diphosphodolichol:dolichyl β-D-mannosyl phosphate mannosyl transferase (mannosyltransferase)

EC Number: 2.4.1.259

a (mannosyl)6-(N-acetylglucosaminyl)2-diphosphodolichol + a dolichyl β-D-mannosyl phosphate <=> a (mannosyl)7-(N-acetylglucosaminyl)2-diphosphodolichol + a dolichyl phosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: dolichyl-diphosphooligosaccharide biosynthesis

Summary:
ALG9 mutants accumulate (mannosyl)6-(N-acetylglucosaminyl)2-diphosphodolichol [Cipollo00, Burda96].

The Alg9 gene product is involved in the addition of the seventh and ninth mannose to the lipid-linked oligosaccharide chain [Frank05a].

Citations: [Cipollo01]


References

Burda96: Burda P, te Heesen S, Brachat A, Wach A, Dusterhoft A, Aebi M (1996). "Stepwise assembly of the lipid-linked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: identification of the ALG9 gene encoding a putative mannosyl transferase." Proc Natl Acad Sci U S A 93(14);7160-5. PMID: 8692962

Cipollo00: Cipollo JF, Trimble RB (2000). "The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing." J Biol Chem 275(6);4267-77. PMID: 10660594

Cipollo01: Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N (2001). "The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum." J Biol Chem 276(24);21828-40. PMID: 11278778

Frank05a: Frank CG, Aebi M (2005). "ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis." Glycobiology 15(11);1156-63. PMID: 15987956


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13B.