|Gene:||PNAE||Accession Number: G-6083 (MetaCyc)|
Species: Rauvolfia serpentina
A polyneuridine aldehyde esterase was partially purified from Rauvolfia serpentina. Using the available partial polypeptide sequence, a cDNA clone of the enzyme has been isolated. Sequence indicates the polyneuridine aldehyde esterase belongs to the alpha/beta hydrolase super family. The cDNA was heterologously expressed in E.coli, purified, and the enzyme activity was subsequently assayed. The enzyme has exceptionally high substrate-specificity towards polyneuridine aldehyde. Other structurally similar esters are not accepted.[Dogru00]
A range of plant species was detected for the existence of polyneuridine aldehyde esterase activity. High enzyme activities were only present in species which are known to contain sarpagine, including Rauvolfia serpentina and Rauvolfia vomitoria. Catharanthus roseus only had trace level enzyme activity. It indicates polyneuridine aldehyde esterase occupies a central role in the biosynthesis of sarpagine type alkaloids.[Pfitzner83]
Molecular Weight of Polypeptide: 29.6 kD (from nucleotide sequence), 30 kD (experimental) [Dogru00 ]
pI: 5.9 [Dogru00]
Revised 29-Aug-2006 by Zhang P
Enzymatic reaction of: polyneuridine aldehyde esterase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
In Pathways: ajmaline and sarpagine biosynthesis
Dogru00: Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J (2000). "The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family." Eur J Biochem 267(5);1397-406. PMID: 10691977
Pfitzner83: Pfitzner, Artur, Stockigt, Joachim (1983). "Polyneuridine aldehyde esterase: an unusually specific enzyme involved in the biosynthesis of sarpagine type alkaloids." J. Chem. SOC., Chem. Commun., 459-460.
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