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MetaCyc Polypeptide: Photosystem I iron-sulfur center

Gene: psaC Accession Number: G-414 (MetaCyc)

Synonyms: photosystem I subunit VII, 9 kD polypeptide, PSI-C

Species: Synechocystis sp. PCC 6803

Component of:
photosystem I complex
photosystem I (extended summary available)

Summary:
The gene psaC encodes a 2[4Fe-4S] bacterial ferredoxin type stromal subunit of photosystem I (PSI) [Fischer98a]. It binds the two terminal electron acceptors FA and FB which are both [4Fe-4S] proteins [Fischer98a][Fischer97]. The othermost iron-sulfur cluster of PsaC reduces soluble ferredoxin which binds to PSI via electrostatic interactions [Fischer99a].

Citations: [Kaneko96, Jung97a, Yu95]

Locations: thylakoid membrane

Molecular Weight of Polypeptide: 8.82 kD (from nucleotide sequence)

Unification Links: KEGG:ssl0563 , Protein Model Portal:P32422 , SMR:P32422 , String:1148.ssl0563 , UniProt:P32422

Relationship Links: InterPro:IN-FAMILY:IPR001450 , InterPro:IN-FAMILY:IPR017491 , InterPro:IN-FAMILY:IPR017896 , InterPro:IN-FAMILY:IPR017900 , Pfam:IN-FAMILY:PF12838 , Prosite:IN-FAMILY:PS00198 , Prosite:IN-FAMILY:PS51379

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0042651 - thylakoid membrane

Credits:
Revised 04-May-2011 by Weerasinghe D


Subunit of: photosystem I complex

Species: Synechocystis sp. PCC 6803

Subunit composition of photosystem I complex = [(PsaA)(PsaB)][PsaC][PsaD][PsaE][PsaF][PsaL][PsaJ][PsaK1][PsaL][PsaM][PsaK2]
         P700 protein = (PsaA)(PsaB) (summary available)
                 Photosystem I P700 chlorophyll a apoprotein A1 = PsaA (summary available)
                 Photosystem I P700 chlorophyll a apoprotein A2 = PsaB (summary available)
         Photosystem I iron-sulfur center = PsaC (summary available)
         Photosystem I reaction center subunit II = PsaD (summary available)
         Photosystem I reaction center subunit IV = PsaE (summary available)
         Photosystem I reaction center subunit III = PsaF (summary available)
         Photosystem I reaction center subunit VIII = PsaL (summary available)
         Photosystem I reaction center subunit IX = PsaJ (summary available)
         Photosystem I reaction center subunit PsaK 1 = PsaK1 (summary available)
         Photosystem I reaction center subunit XI = PsaL (summary available)
         Photosystem I reaction center subunit XII = PsaM (summary available)
         Photosystem I reaction center subunit PsaK 2 = PsaK2 (summary available)

Component of: photosystem I (extended summary available)


Subunit of: photosystem I

Synonyms: photosystem I trimer complex

Species: Synechocystis sp. PCC 6803

Subunit composition of photosystem I = [([PsaA][PsaB])(PsaC)(PsaD)(PsaE)(PsaF)(PsaL)(PsaJ)(PsaK1)(PsaL)(PsaM)(PsaK2)]3
         photosystem I complex = ([PsaA][PsaB])(PsaC)(PsaD)(PsaE)(PsaF)(PsaL)(PsaJ)(PsaK1)(PsaL)(PsaM)(PsaK2)
                 P700 protein = (PsaA)(PsaB) (summary available)
                         Photosystem I P700 chlorophyll a apoprotein A1 = PsaA (summary available)
                         Photosystem I P700 chlorophyll a apoprotein A2 = PsaB (summary available)
                 Photosystem I iron-sulfur center = PsaC (summary available)
                 Photosystem I reaction center subunit II = PsaD (summary available)
                 Photosystem I reaction center subunit IV = PsaE (summary available)
                 Photosystem I reaction center subunit III = PsaF (summary available)
                 Photosystem I reaction center subunit VIII = PsaL (summary available)
                 Photosystem I reaction center subunit IX = PsaJ (summary available)
                 Photosystem I reaction center subunit PsaK 1 = PsaK1 (summary available)
                 Photosystem I reaction center subunit XI = PsaL (summary available)
                 Photosystem I reaction center subunit XII = PsaM (summary available)
                 Photosystem I reaction center subunit PsaK 2 = PsaK2 (summary available)

Summary:
Photosystem I (PS I) is a large membrane protein complex that catalyzes the light driven electron transfer from plastocyanin, which is located on the inside of the thylakoids (the lumen), to ferredoxin, which is on the stromal side of the membrane.

Cyanobacterial PS I can exist in both trimeric and monomeric forms. The trimeric form has been shown to be the prominent oligomeric state at low light intensity. The monomeric unit of PSI from Synechocystis sp. PCC 6803 consists of 11 protein subunits, to which 127 cofactors are non-covalently bound. The 12 proteins are Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I P700 chlorophyll a apoprotein A2, Photosystem I iron-sulfur center, Photosystem I reaction center subunit II, Photosystem I reaction center subunit IV, Photosystem I reaction center subunit III, Photosystem I reaction center subunit IX, Photosystem I reaction center subunit IX, Photosystem I reaction center subunit PsaK 1, Photosystem I reaction center subunit XI, Photosystem I reaction center subunit XII. Some thermophilic cyanobacteria may contain an additional subunit, PsaX.

The processes catalyzed by Photosystem I can be divided into:

1. light capturing and excitation energy transfer

2. electron transfer.

The light capturing is performed by the large antenna system that consists of 90 antenna chlorophylls and 22 carotenoids. The excitation energy is transferred to the center of the complex, where the electron transport chain is located. The electron transport chain consists of six chlorophylls, two phylloquinones and three 4Fe4S clusters.

The charge separation is initiated from the P700 protein, the electron donor, which consists of a heterodimer of a chlorophyll a and a chlorophyll a' (the C13 epimer of chlorophyll a). From P700, the electron is transferred stepwise to A (a chlorophyll a molecule), A0 (also a chlorophyll a molecule), A1 (a phylloquinone molecule) and from there subsequently to the three a [4Fe-4S] iron-sulfur cluster, named FX, FA and FB. After the docking of ferredoxin, the electron is transferred from the terminal 4Fe4S cluster of PS I, FB, to the 2Fe2S cluster of ferredoxin, which transfers the electron to the ferredoxin-NADP oxidoreductase, where NADP+ is finally reduced to NADPH. Under conditions of iron limitation, a flavodoxin may replace the ferredoxin [Grotjohann05].

P700 is re-reduced with the electrons delivered from photosystem II by plastocyanin.

Credits:
Created 04-Jan-1999 by Zhang P , TAIR
Revised 23-May-2008 by Caspi R , SRI International


Enzymatic reaction of: photosystem I

EC Number: 1.97.1.12

a reduced plastocyanin + an oxidized ferredoxin + hν <=> an oxidized plastocyanin + a reduced ferredoxin

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: ethylene biosynthesis V (engineered) , photosynthetic 3-hydroxybutyrate biosynthesis (engineered) , oxygenic photosynthesis , photosynthesis light reactions


References

Fischer97: Fischer N, Setif P, Rochaix JD (1997). "Targeted mutations in the psaC gene of Chlamydomonas reinhardtii: preferential reduction of FB at low temperature is not accompanied by altered electron flow from photosystem I to ferredoxin." Biochemistry 36(1);93-102. PMID: 8993322

Fischer98a: Fischer N, Hippler M, Setif P, Jacquot JP, Rochaix JD (1998). "The PsaC subunit of photosystem I provides an essential lysine residue for fast electron transfer to ferredoxin." EMBO J 17(4);849-58. PMID: 9463363

Fischer99a: Fischer N, Setif P, Rochaix JD (1999). "Site-directed mutagenesis of the PsaC subunit of photosystem I. F(b) is the cluster interacting with soluble ferredoxin." J Biol Chem 274(33);23333-40. PMID: 10438510

Grotjohann05: Grotjohann I, Fromme P (2005). "Structure of cyanobacterial photosystem I." Photosynth Res 85(1);51-72. PMID: 15977059

Jung97a: Jung YS, Vassiliev IR, Yu J, McIntosh L, Golbeck JH (1997). "Strains of Synechocystis sp. PCC 6803 with altered PsaC. II. EPR and optical spectroscopic properties of FA and FB in aspartate, serine, and alanine replacements of cysteines 14 and 51." J Biol Chem 272(12);8040-9. PMID: 9065477

Kaneko96: Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E, Nakamura Y, Miyajima N, Hirosawa M, Sugiura M, Sasamoto S, Kimura T, Hosouchi T, Matsuno A, Muraki A, Nakazaki N, Naruo K, Okumura S, Shimpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S (1996). "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." DNA Res 1996;3(3);109-36. PMID: 8905231

Park06: Park YJ, Yoo CB, Choi SY, Lee HB (2006). "Purifications and characterizations of a ferredoxin and its related 2-oxoacid:ferredoxin oxidoreductase from the hyperthermophilic archaeon, Sulfolobus solfataricus P1." J Biochem Mol Biol 39(1);46-54. PMID: 16466637

Yu95: Yu J, Smart LB, Jung YS, Golbeck J, McIntosh L (1995). "Absence of PsaC subunit allows assembly of photosystem I core but prevents the binding of PsaD and PsaE in Synechocystis sp. PCC6803." Plant Mol Biol 29(2);331-42. PMID: 7579183


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14B.