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MetaCyc Enzyme: dTDP-fucosamine acetyltransferase

Gene: rffC Accession Numbers: EG11455 (MetaCyc), b3790, ECK3782

Synonyms: rff, wecD, yifH

Species: Escherichia coli K-12 substr. MG1655

Summary:
dTDP-fucosamine acetyltransferase catalyzes the synthesis of TDP-4-acetamido-4,6-dideoxy-D-galactose, which is utilized in the biosynthesis of Enterobacterial Common Antigen (ECA). Surprisingly little experimental work on this enzyme has been published.

Crystal structures of RffC from the uropathogenic strain CFT073, both in the apo form and in complex with acetyl-CoA, have been solved at 1.95 and 1.66 Å resolution, respectively [Hung06].

Mutants with a defective rffC gene accumulate lipid III [MeierDieter90, MeierDieter92].

Locations: inner membrane

Map Position: [3,972,619 -> 3,973,164]

Molecular Weight of Polypeptide: 19.545 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012382 , EchoBASE:EB1424 , EcoGene:EG11455 , EcoliWiki:b3790 , ModBase:P27832 , OU-Microarray:b3790 , PortEco:rffC , Protein Model Portal:P27832 , RefSeq:YP_026256 , RegulonDB:EG11455 , SMR:P27832 , String:511145.b3790 , UniProt:P27832

Relationship Links: InterPro:IN-FAMILY:IPR000182 , InterPro:IN-FAMILY:IPR002110 , InterPro:IN-FAMILY:IPR012752 , InterPro:IN-FAMILY:IPR016181 , InterPro:IN-FAMILY:IPR020683 , Pfam:IN-FAMILY:PF00583 , Prosite:IN-FAMILY:PS51186

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009246 - enterobacterial common antigen biosynthetic process Inferred from experiment Inferred by computational analysis [GOA01, Rahman01]
GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [UniProtGOA11]
GO:0009243 - O antigen biosynthetic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0008080 - N-acetyltransferase activity Inferred from experiment Inferred by computational analysis [GOA01, Matsuhashi64]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016746 - transferase activity, transferring acyl groups Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005886 - plasma membrane

MultiFun Terms: cell structure surface antigens (ECA, O antigen of LPS)

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: dTDP-fucosamine acetyltransferase

EC Number: 2.3.1.210

dTDP-thomosamine + acetyl-CoA <=> dTDP-N-acetylthomosamine + coenzyme A + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis , enterobacterial common antigen biosynthesis , dTDP-N-acetylthomosamine biosynthesis

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

History:
10/20/97 Gene b3790 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11455; confirmed by SwissProt match.


References

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hung06: Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A (2006). "Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis." J Bacteriol 188(15);5606-17. PMID: 16855251

Matsuhashi64: Matsuhashi M, Strominger JL (1964). "Thymidine Diphosphate 4-Acetamido-4, 6-dideoxyhexoses. I. Enzymatic Synthesis by Strains of Escherichia coli." J Biol Chem 239;2454-63. PMID: 14235522

MeierDieter90: Meier-Dieter U, Starman R, Barr K, Mayer H, Rick PD (1990). "Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis." J Biol Chem 1990;265(23);13490-7. PMID: 2166030

MeierDieter92: Meier-Dieter U, Barr K, Starman R, Hatch L, Rick PD (1992). "Nucleotide sequence of the Escherichia coli rfe gene involved in the synthesis of enterobacterial common antigen. Molecular cloning of the rfe-rff gene cluster." J Biol Chem 1992;267(2);746-53. PMID: 1730666

Rahman01: Rahman A, Barr K, Rick PD (2001). "Identification of the structural gene for the TDP-Fuc4NAc:lipid II Fuc4NAc transferase involved in synthesis of enterobacterial common antigen in Escherichia coli K-12." J Bacteriol 183(22);6509-16. PMID: 11673418

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC14A.