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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: folylpolyglutamate synthetase

Gene: DHFS/FPGS4 Accession Number: AT3G55630 (MetaCyc)

Synonyms: At3g55630, AtDFD

Species: Arabidopsis thaliana col

Summary:
The Arabidopsis genome was searched for sequences similar to dihydrofolate synthetase/folylpolyglutamate synthetase (DHFS/FPGS) signatures and four cDNA's were retrieved. The molecular functions of the cDNA's were tested by functional complementing mutant yeasts defect in either DHFS or FPGS activity. Protein extracts from the complemented yeast were used to confirm the specific function of the enzyme in vitro. Three cDNA's encoded for folylpolyglutamate synthetase activity (FPGS) but did not exhibit any dihydrofolate synthetase activity (DHFS). Interestingly, in contrast to any other FPGS analyzed in other organisms, Arabidopsis has a set of FPGS's, which not only have different subcellular locations but are encoded by three different genes. One of them, named AtDFD (At3g55630), encoded for the FPGS located in the cytosol catalyzing the addition of glutamyl residues to the different tetrafolate derivatives. The fact that each compartment of the cell possesses its own, probably transcriptional regulated FPGS supports the view that the unconjugated form of tetrahydrofolate (THF) may represent the transport form of THF which has to be distributed between mitochondria (origin of THF), cytosol and chloroplasts. The FPGS in generating the polyglutamylated isoforms prevent the transport of those different derivatives and process their cell-compartment-dependent biosynthesis [Ravanel01].

Locations: cytosol

Map Position: [20,647,648 -> 20,650,471]

Molecular Weight of Polypeptide: 55.1 kD (from nucleotide sequence)

Unification Links: Entrez:CAC82079 , TAIR:At3g55630

Relationship Links: Entrez-Nucleotide:PART-OF:AJ292545

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005829 - cytosol [Ravanel01]


Enzymatic reaction of: 10-formyltetrahydrofolate:L-glutamate ligase (ADP-forming) (folylpolyglutamate synthetase)

EC Number: 6.3.2.17

10-formyl-tetrahydropteroyl-[γ-Glu](n) + L-glutamate + ATP <=> 10-formyl-tetrahydropteroyl-[γ-Glu](n+1) + ADP + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: folate polyglutamylation


Enzymatic reaction of: tetrahydropteroyl-γ-polyglutamate:L-glutamate γ-ligase (ADP-forming) (folylpolyglutamate synthetase)

EC Number: 6.3.2.17

tetrahydropteroyl-[γ-Glu](n) + L-glutamate + ATP <=> tetrahydropteroyl-[γ-Glu](n+1) + ADP + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: folate polyglutamylation

Citations: [Ravanel01]


References

Ravanel01: Ravanel S, Cherest H, Jabrin S, Grunwald D, Surdin-Kerjan Y, Douce R, Rebeille F (2001). "Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana." Proc Natl Acad Sci U S A 98(26);15360-5. PMID: 11752472


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14B.