|Gene:||ligJ||Accession Number: G-2547 (MetaCyc)|
Synonyms: DMA hydratase, 4-carboxy-2-hydroxymuconate hydratase
Species: Sphingomonas sp. SYK6
Subunit composition of
4-oxalomesaconate hydratase = [LigJ]2
4-oxalomesaconate hydratase monomer = LigJ
The ligJ gene of Sphingomonas sp. SYK6, which encodes 4-oxalomesaconate hydratase, was found in the gene cluster carrying the genes for several enzymes involved in protocatechuate degradation [Hara00]. The gene was cloned and expressed in Escherichia coli, and the recombinant protein was purified to near homogeneity and characterized. The enzyme was found to be a homodimer of 69.5 kDa by gel filtration chromatography. Activity was inhibited by the addition of thiol reagents, suggesting that a cysteine residue is part of the catalytic site.
Molecular Weight of Polypeptide: 38.052 kD (from nucleotide sequence)
Molecular Weight of Multimer: 69.5 kD (experimental) [Hara00]
pI: 4.9 [Hara00]
Enzymatic reaction of: 4-oxalomesaconate hydratase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
This reaction is reversible.
T(opt): 30 °C [Hara00]
Hara00: Hara H, Masai E, Katayama Y, Fukuda M (2000). "The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6." J Bacteriol 182(24);6950-7. PMID: 11092855
Masai99: Masai E, Shinohara S, Hara H, Nishikawa S, Katayama Y, Fukuda M (1999). "Genetic and biochemical characterization of a 2-pyrone-4, 6-dicarboxylic acid hydrolase involved in the protocatechuate 4, 5-cleavage pathway of Sphingomonas paucimobilis SYK-6." J Bacteriol 181(1);55-62. PMID: 9864312
Noda90: Noda Y, Nishikawa S, Shiozuka K, Kadokura H, Nakajima H, Yoda K, Katayama Y, Morohoshi N, Haraguchi T, Yamasaki M (1990). "Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis." J Bacteriol 172(5);2704-9. PMID: 2185230
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