MetaCyc Enzyme: pyruvate flavodoxin oxidoreductase

Gene: nifJ Accession Number: G-370 (MetaCyc)

Species: Klebsiella pneumoniae

Subunit composition of pyruvate flavodoxin oxidoreductase = [NifJ]2

The function of the Klebsiella pneumoniae pyruvate flavodoxin oxidoreductase is to supply electrons to nitrogenase. The oxidoreductase employs a nitrogen fixation-specific flavodoxin, the nifF gene product. The oxidoreductase contains two Fe4S4 clusters [Wahl87, Bogusz81, Shah83].

Molecular Weight of Polypeptide: 128.04 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:X01007 , Entrez-Nucleotide:X13303 , Protein Model Portal:P03833 , UniProt:P03833

Relationship Links: InterPro:IN-FAMILY:IPR001450 , InterPro:IN-FAMILY:IPR002869 , InterPro:IN-FAMILY:IPR002880 , InterPro:IN-FAMILY:IPR009014 , InterPro:IN-FAMILY:IPR011766 , InterPro:IN-FAMILY:IPR011895 , InterPro:IN-FAMILY:IPR017896 , InterPro:IN-FAMILY:IPR017900 , InterPro:IN-FAMILY:IPR019456 , InterPro:IN-FAMILY:IPR019752 , InterPro:IN-FAMILY:IPR029061 , Pfam:IN-FAMILY:PF00037 , Pfam:IN-FAMILY:PF01558 , Pfam:IN-FAMILY:PF01855 , Pfam:IN-FAMILY:PF02775 , Pfam:IN-FAMILY:PF10371 , Prosite:IN-FAMILY:PS00198 , Prosite:IN-FAMILY:PS51379 , Smart:IN-FAMILY:SM00890

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Enzymatic reaction of: pyruvate flavodoxin oxidoreductase

Synonyms: pyruvate:flavodoxin oxidoreductase

EC Number: 1.2.99.-

pyruvate + an oxidized flavodoxin + coenzyme A + H+ <=> acetyl-CoA + CO2 + a reduced flavodoxin

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

Alternative Substrates [Comment 1]:

Cofactors or Prosthetic Groups: a [4Fe-4S] iron-sulfur cluster [Comment 2], thiamin diphosphate [Comment 3]

Inhibitors (Unknown Mechanism): sodium dithionite [Wahl87]


Bogusz81: Bogusz D, Houmard J, Aubert JP (1981). "Electron transport to nitrogenase in Klebsiella pneumoniae: purification and properties of the nifJ protein." Eur J Biochem 1981;120(2);421-6. PMID: 6274638

Shah83: Shah VK, Stacey G, Brill WJ (1983). "Electron transport to nitrogenase. Purification and characterization of pyruvate:flavodoxin oxidoreductase. The nifJ gene product." J Biol Chem 1983;258(19);12064-8. PMID: 6352705

Wahl87: Wahl RC, Orme-Johnson WH (1987). "Clostridial pyruvate oxidoreductase and the pyruvate-oxidizing enzyme specific to nitrogen fixation in Klebsiella pneumoniae are similar enzymes." J Biol Chem 1987;262(22);10489-96. PMID: 3038882

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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