|Gene:||nifJ||Accession Number: G-370 (MetaCyc)|
Species: Klebsiella pneumoniae
Subunit composition of pyruvate flavodoxin oxidoreductase = [NifJ]2
Molecular Weight of Polypeptide: 128.04 kD (from nucleotide sequence) [Bairoch93 ]
Enzymatic reaction of: pyruvate flavodoxin oxidoreductase
Synonyms: pyruvate:flavodoxin oxidoreductase
EC Number: 1.2.99.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
This reaction is reversible.
Alternative Substrates [Comment 1]:
The function of the Klebsiella pneumoniae pyruvate flavodoxin oxidoreductase is to supply electrons to nitrogenase. The oxidoreductase employs a nitrogen fixation-specific flavodoxin, the nifF gene product. The oxidoreductase contains two Fe4S4 clusters. [Wahl87, Shah83, Bogusz81]
Inhibitors (Unknown Mechanism): sodium dithionite [Wahl87]
Bogusz81: Bogusz D, Houmard J, Aubert JP (1981). "Electron transport to nitrogenase in Klebsiella pneumoniae: purification and properties of the nifJ protein." Eur J Biochem 1981;120(2);421-6. PMID: 6274638
Shah83: Shah VK, Stacey G, Brill WJ (1983). "Electron transport to nitrogenase. Purification and characterization of pyruvate:flavodoxin oxidoreductase. The nifJ gene product." J Biol Chem 1983;258(19);12064-8. PMID: 6352705
Wahl87: Wahl RC, Orme-Johnson WH (1987). "Clostridial pyruvate oxidoreductase and the pyruvate-oxidizing enzyme specific to nitrogen fixation in Klebsiella pneumoniae are similar enzymes." J Biol Chem 1987;262(22);10489-96. PMID: 3038882
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