|Gene:||MTH_552||Accession Number: G-11078 (MetaCyc)|
Subunit composition of
phosphoglycerol geranylgeranyltransferase = [MTH_552]5
phosphoglycerol geranylgeranyltransferase = MTH_552
phosphoglycerol geranylgeranyltransferase is a cytosolic enzyme that catalyzes the transfer of a geranylgeranyl unit from geranylgeranyl diphosphate to the C3 hydroxyl of sn-glycerol 1-phosphate, forming 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate.
Enzymatic activity has been documented in cell-free extract of Methanothermobacter thermautotrophicus [Zhang90]. The enzyme has been subsequently isolated and purified to homogeneity [Chen93]. It requires Mg2+ for optimal activity, although activity was also observed in buffers containing Mn2+ or Zn2+.
The gene encoding the enzyme was cloned and expressed in Escherichia coli and the recombinant enzyme was purified to homogeneity [Soderberg01]. The enzyme was found to be active as a homopentamer, with optimal activity at 55 degrees C and pH 8.0 [Soderberg01].
Molecular Weight of Polypeptide: 26.119 kD (from nucleotide sequence), 29.0 kD (experimental) [Chen93 ]
Molecular Weight of Multimer: 137.0 kD (experimental) [Soderberg01]
|Cellular Component:||GO:0005829 - cytosol [Zhang90]|
Enzymatic reaction of: phosphoglycerol geranylgeranyltransferase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
Primary Physiological Regulators of Enzyme Activity: Mg2+
T(opt): 55 °C [Soderberg01]
pH(opt): 6-7.5 [Chen93]
Chen93: Chen A, Zhang D, Poulter CD (1993). "(S)-geranylgeranylglyceryl phosphate synthase. Purification and characterization of the first pathway-specific enzyme in archaebacterial membrane lipid biosynthesis." J Biol Chem 268(29);21701-5. PMID: 8408023
Soderberg01: Soderberg T, Chen A, Poulter CD (2001). "Geranylgeranylglyceryl phosphate synthase. Characterization of the recombinant enzyme from Methanobacterium thermoautotrophicum." Biochemistry 40(49);14847-54. PMID: 11732904
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