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discounted EARLY registration ends Dec 31, 2014
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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: Carbon monoxide accessory protein CooCTJ

Species: Rhodospirillum rubrum

Subunit composition of Carbon monoxide accessory protein CooCTJ = [CooJ][CooT][CooC]

Summary:
Background

During anaerobic growth in the presence of CO, the phototrophic bacterium Rhodospirillum rubrum expresses a [NiFe] carbon monoxide dehydrogenase (CODH) which couples CO oxidation to H2 production. CODH genes are encoded in the cooFSCTJ operon and entail the structural gene products of CooS and CooF for a complete enzyme complex. A unique [Ni4Fe4S] cluster, known as the C cluster, constitutes the active site of the enzyme [Jeon05].

About this Protein

The gene products of CooCTJ are involved in Ni insertion into the CODH and are located downstream of the CODH structural gene (CooS) [Kerby97]. The products of cooC and cooJ are required for normal Ni processing into the apo-CODH during CO dependent growth. The Ni-deficient apo form of CODH accumulates in Rhodospirillum rubrum in Ni-deficient medium, but this form can be readily activated in the presence of Ni [Jeon05]. This activation of apo-CODH involves the rapid formation of an inactive apo-CODH-Ni complex prior to conversion to the active holo-CODH [Jeon05].

Gene-Reaction Schematic: ?

Credits:
Created 31-Mar-2011 by Weerasinghe D , SRI International


Enzymatic reaction of: nickel insertion (Carbon monoxide accessory protein CooCTJ)

an apo carbon monoxide dehydrogenase + Ni2+ <=> a holo carbon monoxide dehydrogenase

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Subunit of Carbon monoxide accessory protein CooCTJ: CooJ

Gene: cooJ Accession Numbers: G-12730 (MetaCyc), A

Unification Links: UniProt:P72321


Subunit of Carbon monoxide accessory protein CooCTJ: CooT

Gene: cooT Accession Number: G-12729 (MetaCyc)

Unification Links: UniProt:P72320

Relationship Links: InterPro:IN-FAMILY:IPR019300 , Pfam:IN-FAMILY:PF10133


Subunit of Carbon monoxide accessory protein CooCTJ: CooC

Gene: cooC Accession Number: G-12728 (MetaCyc)

Unification Links: Protein Model Portal:P31897 , UniProt:P31897

Relationship Links: InterPro:IN-FAMILY:IPR002586 , InterPro:IN-FAMILY:IPR014433 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF01656


References

Jeon05: Jeon WB, Singer SW, Ludden PW, Rubio LM (2005). "New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster." J Biol Inorg Chem 10(8);903-12. PMID: 16283394

Kerby97: Kerby RL, Ludden PW, Roberts GP (1997). "In vivo nickel insertion into the carbon monoxide dehydrogenase of Rhodospirillum rubrum: molecular and physiological characterization of cooCTJ." J Bacteriol 179(7);2259-66. PMID: 9079911


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13A.