MetaCyc Enzyme: Carbon monoxide accessory protein CooCTJ

Species: Rhodospirillum rubrum

Subunit composition of Carbon monoxide accessory protein CooCTJ = [CooJ][CooT][CooC]


During anaerobic growth in the presence of CO, the phototrophic bacterium Rhodospirillum rubrum expresses a [NiFe] carbon monoxide dehydrogenase (CODH) which couples CO oxidation to H2 production. CODH genes are encoded in the cooFSCTJ operon and entail the structural gene products of CooS and CooF for a complete enzyme complex. A unique [Ni4Fe4S] cluster, known as the C cluster, constitutes the active site of the enzyme [Jeon05].

About this Protein

The gene products of CooCTJ are involved in Ni insertion into the CODH and are located downstream of the CODH structural gene (CooS) [Kerby97]. The products of cooC and cooJ are required for normal Ni processing into the apo-CODH during CO dependent growth. The Ni-deficient apo form of CODH accumulates in Rhodospirillum rubrum in Ni-deficient medium, but this form can be readily activated in the presence of Ni [Jeon05]. This activation of apo-CODH involves the rapid formation of an inactive apo-CODH-Ni complex prior to conversion to the active holo-CODH [Jeon05].

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 31-Mar-2011 by Weerasinghe D, SRI International

Enzymatic reaction of: nickel insertion (Carbon monoxide accessory protein CooCTJ)

Inferred from experiment

an apo-[carbon monoxide dehydrogenase] + Ni2+ ⇄ a holo-[carbon monoxide dehydrogenase]

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Subunit of Carbon monoxide accessory protein CooCTJ: CooJ

Gene: cooJ Accession Numbers: G-12730 (MetaCyc), A

Unification Links: UniProt:P72321

Subunit of Carbon monoxide accessory protein CooCTJ: CooT

Gene: cooT Accession Number: G-12729 (MetaCyc)

Unification Links: UniProt:P72320

Relationship Links: InterPro:IN-FAMILY:IPR019300, Pfam:IN-FAMILY:PF10133

Subunit of Carbon monoxide accessory protein CooCTJ: CooC

Gene: cooC Accession Number: G-12728 (MetaCyc)

Unification Links: Protein Model Portal:P31897, UniProt:P31897

Relationship Links: InterPro:IN-FAMILY:IPR002586, InterPro:IN-FAMILY:IPR014433, InterPro:IN-FAMILY:IPR027417, Pfam:IN-FAMILY:PF01656


Jeon05: Jeon WB, Singer SW, Ludden PW, Rubio LM (2005). "New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster." J Biol Inorg Chem 10(8);903-12. PMID: 16283394

Kerby97: Kerby RL, Ludden PW, Roberts GP (1997). "In vivo nickel insertion into the carbon monoxide dehydrogenase of Rhodospirillum rubrum: molecular and physiological characterization of cooCTJ." J Bacteriol 179(7);2259-66. PMID: 9079911

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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