Species: Rhodospirillum rubrum
Subunit composition of Carbon monoxide accessory protein CooCTJ = [CooJ][CooT][CooC]
During anaerobic growth in the presence of CO, the phototrophic bacterium Rhodospirillum rubrum expresses a [NiFe] carbon monoxide dehydrogenase (CODH) which couples CO oxidation to H2 production. CODH genes are encoded in the cooFSCTJ operon and entail the structural gene products of CooS and CooF for a complete enzyme complex. A unique [Ni4Fe4S] cluster, known as the C cluster, constitutes the active site of the enzyme [Jeon05].
About this Protein
The gene products of CooCTJ are involved in Ni insertion into the CODH and are located downstream of the CODH structural gene (CooS) [Kerby97]. The products of cooC and cooJ are required for normal Ni processing into the apo-CODH during CO dependent growth. The Ni-deficient apo form of CODH accumulates in Rhodospirillum rubrum in Ni-deficient medium, but this form can be readily activated in the presence of Ni [Jeon05]. This activation of apo-CODH involves the rapid formation of an inactive apo-CODH-Ni complex prior to conversion to the active holo-CODH [Jeon05].
Enzymatic reaction of: nickel insertion (Carbon monoxide accessory protein CooCTJ)
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Reversibility of this reaction is unspecified.
|Gene:||cooJ||Accession Numbers: G-12730 (MetaCyc), A|
Unification Links: UniProt:P72321
|Gene:||cooT||Accession Number: G-12729 (MetaCyc)|
Unification Links: UniProt:P72320
|Gene:||cooC||Accession Number: G-12728 (MetaCyc)|
Jeon05: Jeon WB, Singer SW, Ludden PW, Rubio LM (2005). "New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster." J Biol Inorg Chem 10(8);903-12. PMID: 16283394
Kerby97: Kerby RL, Ludden PW, Roberts GP (1997). "In vivo nickel insertion into the carbon monoxide dehydrogenase of Rhodospirillum rubrum: molecular and physiological characterization of cooCTJ." J Bacteriol 179(7);2259-66. PMID: 9079911
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