Species: Rhodospirillum rubrum
Subunit composition of Carbon monoxide accessory protein CooCTJ = [CooJ][CooT][CooC]
During anaerobic growth in the presence of CO, the phototrophic bacterium Rhodospirillum rubrum expresses a [NiFe] carbon monoxide dehydrogenase (CODH) which couples CO oxidation to H2 production. CODH genes are encoded in the cooFSCTJ operon and entail the structural gene products of CooS and CooF for a complete enzyme complex. A unique [Ni4Fe4S] cluster, known as the C cluster, constitutes the active site of the enzyme [Jeon05].
About this Protein
The gene products of CooCTJ are involved in Ni insertion into the CODH and are located downstream of the CODH structural gene (CooS) [Kerby97]. The products of cooC and cooJ are required for normal Ni processing into the apo-CODH during CO dependent growth. The Ni-deficient apo form of CODH accumulates in Rhodospirillum rubrum in Ni-deficient medium, but this form can be readily activated in the presence of Ni [Jeon05]. This activation of apo-CODH involves the rapid formation of an inactive apo-CODH-Ni complex prior to conversion to the active holo-CODH [Jeon05].
Enzymatic reaction of: nickel insertion (Carbon monoxide accessory protein CooCTJ)
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.
Reversibility of this reaction is unspecified.
|Gene:||cooJ||Accession Numbers: G-12730 (MetaCyc), A|
Unification Links: UniProt:P72321
|Gene:||cooT||Accession Number: G-12729 (MetaCyc)|
Unification Links: UniProt:P72320
|Gene:||cooC||Accession Number: G-12728 (MetaCyc)|
Jeon05: Jeon WB, Singer SW, Ludden PW, Rubio LM (2005). "New insights into the mechanism of nickel insertion into carbon monoxide dehydrogenase: analysis of Rhodospirillum rubrum carbon monoxide dehydrogenase variants with substituted ligands to the [Fe3S4] portion of the active-site C-cluster." J Biol Inorg Chem 10(8);903-12. PMID: 16283394
Kerby97: Kerby RL, Ludden PW, Roberts GP (1997). "In vivo nickel insertion into the carbon monoxide dehydrogenase of Rhodospirillum rubrum: molecular and physiological characterization of cooCTJ." J Bacteriol 179(7);2259-66. PMID: 9079911
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