|Gene:||GNA1||Accession Number: AT5G15770 (MetaCyc)|
Species: Arabidopsis thaliana col
Subunit composition of
glucosamine-6-phosphate acetyltransferase = [GNA1]2
glucosamine 6-phosphate N-acetyltransferase = GNA1
GNA1 is a glucosamine-6-phosphate (GlcN6P) N-acetyltransferase that contributes to the synthesis of UDP-N-acetyl-α-D-glucosamine based on in vitro assays [Riegler12]. Expression of a GNA1:GFP fusion protein suggests that the protein is localized to the endoplasmic reticulum where it likely plays a role in protein glycosylation and GPI anchor biosynthesis. Like GNAs found in other species, the Arabidopsis protein appears to exist as a dimer [Riegler12]. Unlike the case for many Arabidopsis genes, there is no obvious close paralog encoding this important enzyme present in the genome. Therefore, it is unsurprising that GNA1 transcripts can be detected in many different Arabidopsis organs including siliques, flowers, stems, leaves, and roots [Riegler12].
Locations: endoplasmic reticulum
|Map Position: [5,144,096 -> 5,144,545]|
Molecular Weight of Multimer: 40.35 kD (experimental) [Riegler12]
|Cellular Component:||GO:0005783 - endoplasmic reticulum [Riegler12]|
Enzymatic reaction of: glucosamine-6-phosphate acetyltransferase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: UDP-N-acetyl-D-glucosamine biosynthesis II
T(opt): 35 °C [Riegler12]
pH(opt): 7-8 [Riegler12]
Jiang05: Jiang H, Dang L, Wang S, Chen H, Wu Y, Jiang X, Wu P (2005). "A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice." Plant Physiol 138(1);232-42. PMID: 15849305
Riegler12: Riegler H, Herter T, Grishkovskaya I, Lude A, Ryngajllo M, Bolger ME, Essigmann B, Usadel B (2012). "Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana." Biochem J. PMID: 22329777
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