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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: phosphatidylinositide phosphatase SAC2

Gene: INPP5F Accession Number: HS12255 (MetaCyc)

Synonyms: KIAA0966, SAC2, Sac domain-containing inositol phosphatase 2

Species: Homo sapiens

Summary:
The INPP5F (SAC2) gene was identified based on the fact that it contains a Sac domain, a domain associated with inositol polyphosphate phosphatase activity. Unlike other mammalian Sac-containing proteins, this gene has no counterpart in yeast [Minagawa01]. Northern analysis showed that the transcript of this gene was present in every tissue tested, but was especially high in the brain, heart, skeletal muscle, kidney, and placenta.

A cDNA encoding the protein was used to generate a GST-conjugated full-length construct, which was expressed in insect cells and purified [Minagawa01]. The recombinant protein catalyzed a 5-phosphatase reaction only with the two lipid substrates a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate and 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate. These substrates are similar to those of type II inositol phosphate phosphatases, but the enzymne differs from those enzymes by the fact that soluble inositol phosphates are not recognized [Minagawa01].

Citations: [Nagase99]

Gene Citations: [Nagase99]

Map Position: [120,729,293 -> 120,832,474]

Molecular Weight of Polypeptide: 128.41 kD (from nucleotide sequence)

Unification Links: ArrayExpress:Q9Y2H2 , Entrez-Nucleotide:AB023183 , PhosphoSite:Q9Y2H2 , Pride:Q9Y2H2 , Protein Model Portal:Q9Y2H2 , SMR:Q9Y2H2 , String:9606.ENSP00000354519 , UniProt:Q9Y2H2

Relationship Links: InterPro:IN-FAMILY:IPR002013 , InterPro:IN-FAMILY:IPR022158 , Pfam:IN-FAMILY:PF02383 , Pfam:IN-FAMILY:PF12456 , Prosite:IN-FAMILY:PS50275

Gene-Reaction Schematic: ?

Gene Class: UNCLASSIFIED

Credits:
Created 27-Oct-2009 by Caspi R , SRI International


Enzymatic reaction of: phosphatidylinositol 4,5-bisphosphate 5-phosphatase (phosphatidylinositide phosphatase SAC2)

EC Number: 3.1.3.36

a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O <=> a 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: 3-phosphoinositide degradation

Activators (Unknown Mechanism): Mg2+ [Minagawa01]

Kinetic Parameters:

Substrate
Km (μM)
Citations
a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
14.3
[Minagawa01]

pH(opt): 6 [Minagawa01]


Enzymatic reaction of: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase (phosphatidylinositide phosphatase SAC2)

EC Number: 3.1.3.86

1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O <=> a 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: 3-phosphoinositide degradation

Activators (Unknown Mechanism): Mg2+ [Minagawa01]

pH(opt): 6 [Minagawa01]

Exons/Introns:


References

Lecompte08: Lecompte O, Poch O, Laporte J (2008). "PtdIns5P regulation through evolution: roles in membrane trafficking?." Trends Biochem Sci 33(10);453-60. PMID: 18774718

Minagawa01: Minagawa T, Ijuin T, Mochizuki Y, Takenawa T (2001). "Identification and characterization of a sac domain-containing phosphoinositide 5-phosphatase." J Biol Chem 276(25);22011-5. PMID: 11274189

Nagase99: Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." DNA Res 6(1);63-70. PMID: 10231032

Rohde02: Rohde G, Wenzel D, Haucke V (2002). "A phosphatidylinositol (4,5)-bisphosphate binding site within mu2-adaptin regulates clathrin-mediated endocytosis." J Cell Biol 158(2);209-14. PMID: 12119359


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14B.