Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: UDPG:nicotinate N-glucosyltransferase

Species: Petroselinum crispum

Summary:
The enzyme catalyzing the convertion of nicotinate to its N-glucosyl conjugate has been partially purified from cell cultures of Petroselinum crispum. N-glucosylnicotinate was proposed to serve as a storage compound supported by the corresponding enzyme activity being present during the entire growth circle and the relatively short half life of about 24 hours. The enzyme, i.e. UDPG:nicotinate N-glucosyltransferase has been shown to promote free reversibility of the reaction indicating a point of regulation for the pyridine nucleotide cycling in plants and highlights the high group-transfer potential of N-glucosylnicotinate [Upmeier88].

Molecular Weight of Polypeptide: 46 kD (experimental) [Upmeier88 ]

Gene-Reaction Schematic: ?

Credits:
Created 25-Oct-2006 by Foerster H , TAIR


Enzymatic reaction of: UDPG:nicotinate N-glucosyltransferase

EC Number: 2.4.1.196

UDP-α-D-glucose + nicotinate + H+ <=> N-glucosylnicotinate + UDP

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

In Pathways: N-glucosylnicotinate metabolism

Inhibitors (Unknown Mechanism): Zn2+ [Upmeier88] , p-chloromercuribenzoate

Kinetic Parameters:

Substrate
Km (μM)
Citations
UDP
1200.0
[Upmeier88]
nicotinate
170.0
[Upmeier88]

T(opt): 30 °C [Upmeier88]

pH(opt): 7.8-8.2 [Upmeier88]


References

Upmeier88: Upmeier B, Thomzik JE, Barz W (1988). "Enzymatic studies on the reversible synthesis of nicotinic acid-N-glucoside in heterotrophic parsley cell suspension cultures." Z. Naturforsch. 43c, 835-842.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, biocyc14.