Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: costunolide synthase

Species: Cichorium intybus

Summary:
An enzyme was purified from cell-free extract of Cichorium intybus by GC-MS. The enzyme was shown to catalyze the conversion of germacra-1(10),4,11(13)-trien-12-oate to (+)-costunolide via 6α-hydroxy-germacra-1(10),4,11(13)-trien-12-oate by lactonization [deKraker02]. Costunolide synthase is dependent on oxygen and NADPH, indicating the involvement of cytochrome P450, confirmed by blue light-reversible inhibition of the enzyme activity by carbon monoxide [deKraker02].

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0016106 - sesquiterpenoid biosynthetic process Inferred from experiment [deKraker02]

Credits:
Created 10-Dec-2007 by Pujar A , Cornell University
Revised 08-May-2014 by Foerster H , Boyce Thompson Institute


Enzymatic reaction of: costunolide synthase

EC Number: 1.14.13.120

germacra-1(10),4,11(13)-trien-12-oate + NADPH + oxygen + H+ <=> 6α-hydroxy-germacra-1(10),4,11(13)-trien-12-oate + NADP+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: costunolide biosynthesis


Enzymatic reaction of: costunolide synthase

Synonyms: (+)-costunolide synthase

EC Number: 1.14.13.120

germacra-1(10),4,11(13)-trien-12-oate + NADPH + oxygen + 2 H+ <=> (+)-costunolide + NADP+ + 2 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: costunolide biosynthesis


References

deKraker02: de Kraker JW, Franssen MC, Joerink M, de Groot A, Bouwmeester HJ (2002). "Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome p450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory." Plant Physiol 129(1);257-68. PMID: 12011356


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.