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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: costunolide synthase

Species: Cichorium intybus

Summary:
An enzyme was purified from cell-free extract of Cichorium intybus by GC-MS. The enzyme was shown to catalyze the conversion of germacra-1(10),4,11(13)-trien-12-oate to (+)-costunolide via 6α-hydroxy-germacra-1(10),4,11(13)-trien-12-oate by lactonization [deKraker02]. Costunolide synthase is dependent on oxygen and NADPH, indicating the involvement of cytochrome P450, confirmed by blue light-reversible inhibition of the enzyme activity by carbon monoxide [deKraker02].

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0016106 - sesquiterpenoid biosynthetic process Inferred from experiment [deKraker02]

Credits:
Created 10-Dec-2007 by Pujar A , Cornell University
Revised 08-May-2014 by Foerster H , Boyce Thompson Institute


Enzymatic reaction of: costunolide synthase

EC Number: 1.14.13.120

germacra-1(10),4,11(13)-trien-12-oate + NADPH + oxygen + H+ <=> 6α-hydroxy-germacra-1(10),4,11(13)-trien-12-oate + NADP+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: costunolide biosynthesis


Enzymatic reaction of: costunolide synthase

Synonyms: (+)-costunolide synthase

EC Number: 1.14.13.120

germacra-1(10),4,11(13)-trien-12-oate + NADPH + oxygen + 2 H+ <=> (+)-costunolide + NADP+ + 2 H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: costunolide biosynthesis


References

deKraker02: de Kraker JW, Franssen MC, Joerink M, de Groot A, Bouwmeester HJ (2002). "Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome p450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory." Plant Physiol 129(1);257-68. PMID: 12011356


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc11.