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discounted EARLY registration ends Dec 31, 2014
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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: alcaligin synthetase

Gene: alcC Accession Numbers: G-11646 (MetaCyc), BB3895

Species: Bordetella bronchiseptica

Summary:
The alcC gene is located in the alacaligin biosynthetic cluster and is involved in alcaligin synthesis [Kang96a, Giardina97]. The gene is highly homologous to several genes involved in the biosynthesis of siderophores in other bacteria, including iucC, pubC and desD. A transposon mutant with insertion into the alcC gene was not able to produce alcaligin [Pradel98].

Although the product of this gene has not been characterized biochemically yet, it is is proposed to catalyze the ATP-mediated condensation and cyclization of two 3-[4-amino-3-hydroxybutyl]-hydroxy-carbamoyl-propanoate monomers into alcaligin, via the free intermediate pre-alcaligin. The mutation could be complemented by introduction of the wild type gene [Challis05].

Molecular Weight of Polypeptide: 69.973 kD (from nucleotide sequence), 59.0 kD (experimental) [Pradel98 ]

Unification Links: Entrez-gene:2661347 , String:257310.BB3895 , UniProt:P94255

Relationship Links: InterPro:IN-FAMILY:IPR007310 , InterPro:IN-FAMILY:IPR022770 , PDB:Structure:2X0O , PDB:Structure:2X0P , PDB:Structure:2X0Q , Pfam:IN-FAMILY:PF04183 , Pfam:IN-FAMILY:PF06276

Gene-Reaction Schematic: ?

Credits:
Created 13-Nov-2009 by Caspi R , SRI International


Enzymatic reaction of: pre-alcaligin cyclase (alcaligin synthetase)

pre-alcaligin + ATP <=> alcaligin + AMP + diphosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: alcaligin biosynthesis


Enzymatic reaction of: pre-alcaligin synthetase

2 3-[4-amino-3-hydroxybutyl]-hydroxy-carbamoyl-propanoate + ATP <=> pre-alcaligin + AMP + diphosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: alcaligin biosynthesis


References

Challis05: Challis GL (2005). "A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases." Chembiochem 6(4);601-11. PMID: 15719346

Giardina97: Giardina PC, Foster LA, Toth SI, Roe BA, Dyer DW (1997). "Analysis of the alcABC operon encoding alcaligin biosynthesis enzymes in Bordetella bronchiseptica." Gene 194(1);19-24. PMID: 9266668

Kang96a: Kang HY, Brickman TJ, Beaumont FC, Armstrong SK (1996). "Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes." J Bacteriol 178(16);4877-84. PMID: 8759851

Pradel98: Pradel E, Guiso N, Locht C (1998). "Identification of AlcR, an AraC-type regulator of alcaligin siderophore synthesis in Bordetella bronchiseptica and Bordetella pertussis." J Bacteriol 180(4);871-80. PMID: 9473041


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.