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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: germacrene A alcohol dehydrogenase/hydroxylase

Gene: GAO1 Accession Number: G-12100 (MetaCyc)

Species: Barnadesia spinosa

Summary:
A cDNA clone was isolated from Barnadesia spinosa plant. The cDNA was recombinantly expressed in yeast. The expressed protein shares identity with GAO1 of Asteraceae. The gene encodes a polypeptide of 488 amino acids. The enzyme synthesizes germcrene A acid de novo [Nguyen10b]. This enzyme catalyzes all three steps to the formation germacrene A acid [Nguyen10b].

Molecular Weight of Polypeptide: 55.7 kD (from nucleotide sequence), 55.7 kD (experimental) [Nguyen10b ]

Unification Links: Entrez-Nucleotide:GU256647 , Entrez:ADF43083 , UniProt:D5JBX1

Relationship Links: InterPro:IN-FAMILY:IPR001128 , InterPro:IN-FAMILY:IPR002401 , InterPro:IN-FAMILY:IPR017972 , Pfam:IN-FAMILY:PF00067 , Prints:IN-FAMILY:PR00385 , Prints:IN-FAMILY:PR00463 , Prosite:IN-FAMILY:PS00086

Gene-Reaction Schematic: ?

Credits:
Created 08-Jul-2010 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: germacrene A alcohol dehydrogenase

EC Number: 1.1.1.314

germacra-1(10),4,11(13)-trien-12-ol + 2 NADP+ + H2O <=> germacra-1(10),4,11(13)-trien-12-oate + 2 NADPH + 3 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

Reversibility of this reaction is unspecified.

In Pathways: costunolide biosynthesis


Enzymatic reaction of: germacrene A oxidase (germacrene A alcohol dehydrogenase/hydroxylase)

germacra-1(10),4,11(13)-trien-12-al + NADP+ + H2O <=> germacra-1(10),4,11(13)-trien-12-oate + NADPH + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: costunolide biosynthesis


Enzymatic reaction of: germacrene A oxidase (germacrene A alcohol dehydrogenase/hydroxylase)

germacra-1(10),4,11(13)-trien-12-ol + NADP+ <=> germacra-1(10),4,11(13)-trien-12-al + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: costunolide biosynthesis


Enzymatic reaction of: germacrene A oxidase (germacrene A alcohol dehydrogenase/hydroxylase)

EC Number: 1.14.13.123

(+)-germacrene A + NADPH + H+ + oxygen <=> germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: costunolide biosynthesis


References

Nguyen10b: Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK (2010). "Biochemical conservation and evolution of germacrene A oxidase in asteraceae." J Biol Chem 285(22);16588-98. PMID: 20351109


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13B.