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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: resveratrol 3,5-O-dimethyltransferase

Gene: ROMT Accession Number: G-12328 (MetaCyc)

Species: Vitis vinifera

Summary:
A cDNA clone was isolated from Vitis vinifera and recombinantly expressed in E.coli. The expressed protein was biochemically characterized, additionally it was co-expressed along with grapevine stilbene synthase in Tobacco using the agroinfiltration method. This gene encoded an enzyme that catalyzes the formation of pterostilbene from resveratrol, both in vitro as well as in planta [Schmidlin08].

Molecular Weight of Polypeptide: 40.08 kD (from nucleotide sequence), 40.0 kD (experimental) [Schmidlin08 ]

Unification Links: Entrez-Nucleotide:FM178870 , Entrez:CAQ76879 , Protein Model Portal:B6VJS4 , UniProt:B6VJS4

Relationship Links: InterPro:IN-FAMILY:IPR001077 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR012967 , InterPro:IN-FAMILY:IPR016461 , Pfam:IN-FAMILY:PF00891 , Pfam:IN-FAMILY:PF08100 , Prosite:IN-FAMILY:PS51683

Gene-Reaction Schematic: ?

Credits:
Created 02-Dec-2010 by Pujar A , Boyce Thompson Institute


Enzymatic reaction of: resveratrol 3,5-O-dimethyltransferase

EC Number: 2.1.1.240

2 S-adenosyl-L-methionine + trans-resveratrol <=> 2 S-adenosyl-L-homocysteine + pterostilbene + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: pterostilbene biosynthesis


Enzymatic reaction of: 3-methoxy-4',5-dihydroxy-trans-stilbene O-methyltransferase (resveratrol 3,5-O-dimethyltransferase)

3-methoxy-4',5-dihydroxy-trans-stilbene + S-adenosyl-L-methionine <=> pterostilbene + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: pterostilbene biosynthesis


Enzymatic reaction of: resveratrol O-methyltransferase (resveratrol 3,5-O-dimethyltransferase)

trans-resveratrol + S-adenosyl-L-methionine <=> 3-methoxy-4',5-dihydroxy-trans-stilbene + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: pterostilbene biosynthesis

Summary:
A cDNA clone was isolated from Vitis and recombinantly expressed in E.coli. The expressed protein was biocehmically characterized, additionally it was co-expressed along with grapevine stilbene synthase in Tobacco, by the agroinfiltration method. This gene encoded an enzyme that catalyzes the formation of pterostilbene from resveratrol in vitro as well as in planta [Schmidlin08].


References

Schmidlin08: Schmidlin L, Poutaraud A, Claudel P, Mestre P, Prado E, Santos-Rosa M, Wiedemann-Merdinoglu S, Karst F, Merdinoglu D, Hugueney P (2008). "A stress-inducible resveratrol O-methyltransferase involved in the biosynthesis of pterostilbene in grapevine." Plant Physiol 148(3);1630-9. PMID: 18799660


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, BIOCYC13A.