MetaCyc Enzyme: 3-oxocholest-4-en-26-oate–CoA ligase

Gene: fadD19 Accession Number: G-14719 (MetaCyc)

Species: Rhodococcus rhodochrous

An ATP-dependent steroid-CoA ligase (65 kDa) from Mycobacterium sp. strain NRRL B3805 was purified to near homogeneity and shown to be highly specific toward C26 carboxylic sterols [Chen85]. However, the gene encoding the enzyme has not been identified at the time.

Deletion of the fadD19 gene of Rhodococcus rhodochrous strain RG32 completely blocked side chain degradation of C-24 branched sterols. Heterologously expressed enzyme was also active toward steroid-C26-oic acid substrates, identifying FadD19 as a steroid-coenzyme A ligase with an essential in vivo role in the degradation of the side chains of C-24 branched-chain sterols [Wilbrink11].

Molecular Weight of Polypeptide: 60.602 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:E3UUE6 , UniProt:E3UUE6

Relationship Links: Entrez-Nucleotide:PART-OF:HM588719 , InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR020845 , InterPro:IN-FAMILY:IPR025110 , Pfam:IN-FAMILY:PF00501 , Pfam:IN-FAMILY:PF13193 , Prosite:IN-FAMILY:PS00455

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 05-Oct-2011 by Caspi R , SRI International

Enzymatic reaction of: 3-oxocholest-4-en-26-oate–CoA ligase

Synonyms: cholest-4-en-3-one-27-oate-CoA ligase

EC Number:

(25S)-3-oxocholest-4-en-26-oate + ATP + coenzyme A <=> (25S)-3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of cholesterol degradation I (cholesterol oxidase) , cholesterol degradation to androstenedione I (cholesterol oxidase)


Chen85: Chen, C. S. (1985). Ph.D. thesis. University of Wisconsin, Madison.

Wilbrink11: Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R (2011). "FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains." Appl Environ Microbiol 77(13);4455-64. PMID: 21602385

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, biocyc13.