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discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: 3-oxocholest-4-en-26-oate–CoA ligase

Gene: fadD19 Accession Number: G-14719 (MetaCyc)

Species: Rhodococcus rhodochrous

Summary:
An ATP-dependent steroid-CoA ligase (65 kDa) from Mycobacterium sp. strain NRRL B3805 was purified to near homogeneity and shown to be highly specific toward C26 carboxylic sterols [Chen85]. However, the gene encoding the enzyme has not been identified at the time.

Deletion of the fadD19 gene of Rhodococcus rhodochrous strain RG32 completely blocked side chain degradation of C-24 branched sterols. Heterologously expressed enzyme was also active toward steroid-C26-oic acid substrates, identifying FadD19 as a steroid-coenzyme A ligase with an essential in vivo role in the degradation of the side chains of C-24 branched-chain sterols [Wilbrink11].

Molecular Weight of Polypeptide: 60.602 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:E3UUE6 , UniProt:E3UUE6

Relationship Links: Entrez-Nucleotide:PART-OF:HM588719 , InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR020845 , InterPro:IN-FAMILY:IPR025110 , Pfam:IN-FAMILY:PF00501 , Pfam:IN-FAMILY:PF13193 , Prosite:IN-FAMILY:PS00455

Gene-Reaction Schematic: ?

Credits:
Created 05-Oct-2011 by Caspi R , SRI International


Enzymatic reaction of: 3-oxocholest-4-en-26-oate–CoA ligase

Synonyms: cholest-4-en-3-one-27-oate-CoA ligase

EC Number: 6.2.1.f

3-oxocholest-4-en-26-oate + ATP + coenzyme A <=> 3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of cholesterol degradation I (cholesterol oxidase) , cholesterol degradation to androstenedione I (cholesterol oxidase)


References

Chen85: Chen, C. S. (1985). Ph.D. thesis. University of Wisconsin, Madison.

Wilbrink11: Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R (2011). "FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains." Appl Environ Microbiol 77(13);4455-64. PMID: 21602385


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14B.