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MetaCyc Enzyme: C18 Δ9-polyunsaturated fatty acyl-CoA elongase

Gene: ASE1 Accession Number: G-14792 (MetaCyc)

Synonyms: C18 Δ9-fatty acid elongase, Δ9 elongase

Species: Isochrysis galbana

Summary:
The microalga Isochrysis galbana is rich in long chain polyunsaturated fatty acids such as docosahexaenoate [Qi02]. The ASE1 gene, encoding a polyunsaturated fatty acid-elongating activity, was isolated from this organism [Qi02]. Transgenic Saccharomyces cerevisiae expressing ASE1 specifically elongated the C18-Δ9 PUFAs linoleate and α-linolenate to an eicosadienoate and an eicosatrienoate, respectively. This suggests that ASE1 encodes the 3-oxoacyl-CoA synthase component of the elongase, and that it is able to interact with the other members of the yeast elongase complex (see very long chain fatty acid biosynthesis II) [Qi02].

Molecular Weight of Polypeptide: 30.19 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:AF390174 , UniProt:Q8W213

Relationship Links: InterPro:IN-FAMILY:IPR002076 , Panther:IN-FAMILY:PTHR11157 , Pfam:IN-FAMILY:PF01151

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 28-Oct-2011 by Weerasinghe D , SRI International


Enzymatic reaction of: 3-oxo-(11Z,14Z)-icosa-11,14-dienoyl-CoA synthase (C18 Δ9-polyunsaturated fatty acyl-CoA elongase)

malonyl-CoA + linoleoyl-CoA + H+ <=> 3-oxo-(11Z,14Z)-icosa-11,14-dienoyl-CoA + CO2 + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: arachidonate biosynthesis IV (8-detaturase)


Enzymatic reaction of: 3-oxo-icosatrienoyl-CoA synthase (C18 Δ9-polyunsaturated fatty acyl-CoA elongase)

malonyl-CoA + α-linolenoyl-CoA + H+ <=> 3-oxo-icosatrienoyl-CoA + CO2 + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: icosapentaenoate biosynthesis V (Δ8 desaturase)


References

Qi02: Qi B, Beaudoin F, Fraser T, Stobart AK, Napier JA, Lazarus CM (2002). "Identification of a cDNA encoding a novel C18-Delta(9) polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana." FEBS Lett 510(3);159-65. PMID: 11801246


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sun Apr 19, 2015, biocyc13.