MetaCyc Transporter: glucosyl-oleandomycin-exporting ATPase

Gene: oleB Accession Number: G-14879 (MetaCyc)

Species: Streptomyces antibioticus

The OleB protein of Streptomyces antibioticus contains two ATP-binding domains, either of which is sufficient to confer resistance to oleandomycin.

The oleB gene was expressed in Escherichia coli and purified. In addition, a recombinant Streptomyces albus strain expressing both the OleD glycosyltransferase and the OleB protein was capable of glycosylating oleandomycin and secreting the inactive glycosylated molecule. Thus it has been proposed that OleB constitutes the secretion system by which the glycosylated form of oleandomycin is secreted by Streptomyces antibioticus [Olano95].

Molecular Weight of Polypeptide: 61.706 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:L36601 , Protein Model Portal:Q53712 , UniProt:Q53712

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 04-Jan-2012 by Caspi R , SRI International

Enzymatic reaction of: glucosyl-oleandomycin-exporting ATPase

EC Number: 3.6.3.-

Transport reaction diagram for glucosyl-oleandomycin-exporting ATPase

In Pathways: oleandomycin activation/inactivation


Olano95: Olano C, Rodriguez AM, Mendez C, Salas JA (1995). "A second ABC transporter is involved in oleandomycin resistance and its secretion by Streptomyces antibioticus." Mol Microbiol 16(2);333-43. PMID: 7565095

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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