MetaCyc Enzyme: chitobiosyldiphosphodolichol β-mannosyltransferase

Gene: ALG1 Accession Number: G-5561 (MetaCyc)

Synonyms: GDP-mannose: dolichyl diphosphate chitobiose mannosyl transferase

Species: Saccharomyces cerevisiae

Residues 8-24 of the protein contain hydrophobic amino acids, and this region is predicted to form a signal sequence and membrane spanning domain [Albright90]. This region also contains a 13-amino acid consensus sequence thought to be involved in dolichol recognition. This 13-amino acid sequence is also found in the glycosyltransferase products of genes ALG7 and DPM1, and the product of gene SEC59 [Albright89].
ALG1 mutants accumulated (N-acetylglucosaminyl)2-diphosphodolichol [Huffaker83].

Citations: [Couto84, Sharma82]

Gene Citations: [Couto84]

Locations: endoplasmic reticulum

Map Position: [458,866 -> 460,215]

Molecular Weight of Polypeptide: 51.9 kD (from nucleotide sequence)

Unification Links: DIP:DIP-7371N , Entrez-gene:852407 , Mint:MINT-1355815 , ModBase:P16661 , Protein Model Portal:P16661 , String:4932.YBR110W , Swiss-Model:P16661 , UniProt:P16661

Relationship Links: CAZy:IN-FAMILY:GT33 , InterPro:IN-FAMILY:IPR001296 , InterPro:IN-FAMILY:IPR026051 , Panther:IN-FAMILY:PTHR13036 , Pfam:IN-FAMILY:PF00534

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005783 - endoplasmic reticulum [Gao04]

Enzymatic reaction of: chitobiosyldiphosphodolichol β-mannosyltransferase

EC Number:

an (N-acetylglucosaminyl)2-diphosphodolichol + GDP-α-D-mannose <=> a mannosyl-(N-acetylglucosaminyl)2-diphosphodolichol + GDP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: protein N-glycosylation (eukaryotic, high mannose)

The enzyme was partially purified from a solubilized membrane fraction [Sharma82]. Production of mannosyl-(N-acetylglucosaminyl)2-diphosphodolichol was shown in an E. coli expression system [Couto84].

Activators (Unknown Mechanism): Mg2+ [Sharma82]

Inhibitors (Unknown Mechanism): GDP [Sharma82] , GMP [Sharma82]

Kinetic Parameters:

Km (μM)
a mannosyl-(N-acetylglucosaminyl)2-diphosphodolichol

pH(opt): 7.5 [Sharma82]


Albright89: Albright CF, Orlean P, Robbins PW (1989). "A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition." Proc Natl Acad Sci U S A 86(19);7366-9. PMID: 2678101

Albright90: Albright CF, Robbins RW (1990). "The sequence and transcript heterogeneity of the yeast gene ALG1, an essential mannosyltransferase involved in N-glycosylation." J Biol Chem 265(12);7042-9. PMID: 2182636

Burda99: Burda P, Aebi M (1999). "The dolichol pathway of N-linked glycosylation." Biochim Biophys Acta 1426(2);239-57. PMID: 9878760

Couto84: Couto JR, Huffaker TC, Robbins PW (1984). "Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway." J Biol Chem 259(1);378-82. PMID: 6368538

Gao04: Gao XD, Nishikawa A, Dean N (2004). "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum." Glycobiology 14(6);559-70. PMID: 15044395

Huffaker83: Huffaker TC, Robbins PW (1983). "Yeast mutants deficient in protein glycosylation." Proc Natl Acad Sci U S A 80(24);7466-70. PMID: 6369318

Sato01: Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A (2001). "Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis." Genes Cells 6(6);495-506. PMID: 11442630

Sharma82: Sharma CB, Lehle L, Tanner W (1982). "Solubilization and characterization of the initial enzymes of the dolichol pathway from yeast." Eur J Biochem 126(2);319-25. PMID: 6215245

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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