|Gene:||TKPR2||Accession Number: AT1G68540 (MetaCyc)|
Species: Arabidopsis thaliana col
Recombinant proteins of TKPR1 and TKPR2, expressed in E. coli, showed catalytic activity towards tetraketide but not triketide α-pyrones. TKPR2 is more active than TKPR1.
TKPR1 and TKPR2 are specifically expressed in tapetum cells and they are tightly co-expressed with ACOS5, PKSA and PKSB. Mutants of TKPR1 or TKPR2 showed defects in pollen exine layer [Grienenberger10].
Instance reaction of [a polyhydroxylated tetraketide α-pyrone + NADP+ = a hydroxylated tetraketide α-pyrone + NADPH + H+] (1.1.1.-):
Enzymatic reaction of: tetraketide α-pyrone reductase
EC Number: 1.1.1.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
Reversibility of this reaction is unspecified.
In Pathways: sporopollenin precursors biosynthesis
Grienenberger10: Grienenberger E, Kim SS, Lallemand B, Geoffroy P, Heintz D, Souza Cde A, Heitz T, Douglas CJ, Legrand M (2010). "Analysis of TETRAKETIDE α-PYRONE REDUCTASE function in Arabidopsis thaliana reveals a previously unknown, but conserved, biochemical pathway in sporopollenin monomer biosynthesis." Plant Cell 22(12);4067-83. PMID: 21193572
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