Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: acyl-CoA synthetase

Gene: LACS9 Accession Number: AT1G77590 (MetaCyc)

Synonyms: At1G77590

Species: Arabidopsis thaliana col

Summary:
The Arabidopsis genome has nine functional genes encoding acyl-CoA synthetase [Shockey02]. Genetic and biochemical evidence have sorted physiological roles for some of them. LACS9 is the major chloroplast acyl-CoA synthetase [Schnurr02]. It contributes to the activation of de novo synthesized fatty acids during their export from the chloroplast. The enzyme is located on the chloroplast envelope. Knockout mutant plants contain only 10% of the chloroplast long-chain acyl-CoA synthetase activity. LACS9 is highly expressed in developing seeds and young rosette leaves.

Locations: plastid envelope

Unification Links: PhylomeDB:Q9CAP8 , Phytozome Plant Orthologs:AT1G77590.1 , Pride:Q9CAP8 , Protein Model Portal:Q9CAP8 , SMR:Q9CAP8 , String:3702.AT1G77590.1-P , TAIR:AT1G77590 , UniProt:Q9CAP8

Relationship Links: InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR020845 , Pfam:IN-FAMILY:PF00501 , Prosite:IN-FAMILY:PS00455

Gene-Reaction Schematic: ?

Instance reaction of [a long-chain fatty acid + ATP + coenzyme A → a long-chain acyl-CoA + AMP + diphosphate] (6.2.1.3):
i1: palmitate + ATP + coenzyme A → palmitoyl-CoA + AMP + diphosphate (6.2.1.3)

GO Terms:

Cellular Component: GO:0009526 - plastid envelope [Schnurr02]


Enzymatic reaction of: long-chain-fatty-acid-CoA ligase (acyl-CoA synthetase)

EC Number: 6.2.1.3

a long-chain fatty acid + ATP + coenzyme A <=> a long-chain acyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: fatty acid activation


Enzymatic reaction of: oleate:CoA ligase (AMP-forming) (acyl-CoA synthetase)

EC Number: 6.2.1.3

oleate + ATP + coenzyme A <=> oleoyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: linoleate biosynthesis I (plants)


Enzymatic reaction of: long-chain-fatty-acid-CoA ligase (acyl-CoA synthetase)

EC Number: 6.2.1.3

a long-chain fatty acid + ATP + coenzyme A <=> a long-chain acyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: fatty acid activation

Summary:
The Arabidopsis genome has nine functional genes encoding acyl-CoA synthetase[Shockey02]. Genetic and biochemical evidence have sorted physiological roles for some of them. LACS9 is the major chloroplast acyl-CoA synthetase[Schnurr02]. It contributes to the activation of de novo synthesized fatty acids during their exporting from the chloroplast. The enzyme is located on the chloroplast envelope. Knockout mutant plants contain only 10% of the chloroplast long-chain acyl-CoA synthetase activity. LACS9 is highly expressed in developing seeds and young rosette leaves.


References

Schnurr02: Schnurr JA, Shockey JM, de Boer GJ, Browse JA (2002). "Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis." Plant Physiol 129(4);1700-9. PMID: 12177483

Shockey02: Shockey JM, Fulda MS, Browse JA (2002). "Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism." Plant Physiol 129(4);1710-22. PMID: 12177484


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, BIOCYC14A.