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MetaCyc Enzyme: long-chain acyl-CoA synthetase 9

Gene: LACS9 Accession Number: AT1G77590 (MetaCyc)

Synonyms: At1G77590

Species: Arabidopsis thaliana col

Summary:
The Arabidopsis genome contains nine functional genes encoding long-chain acyl-CoA synthetase - LACS1, LACS2, LACS3, LACS4, LACS5, LACS6, LACS7, LACS8 and LACS9 [Shockey02]. Genetic and biochemical evidence have sorted the physiological roles of some of them.

LACS9 is the major chloroplast acyl-CoA synthetase [Schnurr02]. It contributes to the activation of de novo synthesized fatty acids during their export from the chloroplast. The enzyme is located on the chloroplast envelope. Knockout mutant plants contain only 10% of the chloroplast long-chain acyl-CoA synthetase activity. LACS9 is highly expressed in developing seeds and young rosette leaves.

Locations: plastid envelope

Unification Links: PhylomeDB:Q9CAP8 , Phytozome Plant Orthologs:AT1G77590.1 , Pride:Q9CAP8 , Protein Model Portal:Q9CAP8 , SMR:Q9CAP8 , String:3702.AT1G77590.1-P , TAIR:AT1G77590 , UniProt:Q9CAP8

Relationship Links: InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR020845 , Pfam:IN-FAMILY:PF00501 , Prosite:IN-FAMILY:PS00455

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reactions of [a long-chain fatty acid + ATP + coenzyme A → a long-chain acyl-CoA + AMP + diphosphate] (6.2.1.3):
i1: palmitate + ATP + coenzyme A → palmitoyl-CoA + AMP + diphosphate (6.2.1.3)

i2: stearate + ATP + coenzyme A → stearoyl-CoA + AMP + diphosphate (6.2.1.3)

i3: 18-hydroxyoleate + ATP + coenzyme A → 18-hydroxyoleoyl-CoA + AMP + diphosphate (6.2.1.3)

i4: 16-hydroxypalmitate + ATP + coenzyme A → 16-hydroxypalmitoyl-CoA + AMP + diphosphate (6.2.1.3)

i5: 18-hydroxystearate + ATP + coenzyme A → 18-hydroxystearoyl-CoA + AMP + diphosphate (6.2.1.3)

i6: an ω-hydroxy long-chain fatty acid + ATP + coenzyme A → an ω-hydroxy long-chain fatty acyl-CoA + AMP + diphosphate (6.2.1.-)

GO Terms:

Cellular Component: GO:0009526 - plastid envelope [Schnurr02]


Enzymatic reaction of: long-chain-fatty-acid-CoA ligase (long-chain acyl-CoA synthetase 9)

EC Number: 6.2.1.3

a long-chain fatty acid + ATP + coenzyme A <=> a long-chain acyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: suberin monomers biosynthesis , stearate biosynthesis II (bacteria and plants) , superpathway of fatty acid biosynthesis II (plant) , palmitate biosynthesis II (bacteria and plants) , long-chain fatty acid activation


Enzymatic reaction of: oleate:CoA ligase (long-chain acyl-CoA synthetase 9)

EC Number: 6.2.1.3

oleate + ATP + coenzyme A <=> oleoyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of fatty acid biosynthesis II (plant) , oleate biosynthesis I (plants)


References

Schnurr02: Schnurr JA, Shockey JM, de Boer GJ, Browse JA (2002). "Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis." Plant Physiol 129(4);1700-9. PMID: 12177483

Shockey02: Shockey JM, Fulda MS, Browse JA (2002). "Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism." Plant Physiol 129(4);1710-22. PMID: 12177484


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat Aug 1, 2015, biocyc11.