Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015 (Sat)
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015 (Sat)
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015 (Sat)
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015 (Sat)
Pathway Tools
Intro Tutorial
discounted registration ends Sept 5, 2015 (Sat)
twitter

MetaCyc Enzyme: long-chain acyl-CoA synthetase 9

Gene: LACS9 Accession Number: AT1G77590 (MetaCyc)

Synonyms: At1G77590

Species: Arabidopsis thaliana col

Summary:
The Arabidopsis genome contains nine functional genes encoding long-chain acyl-CoA synthetase - LACS1, LACS2, LACS3, LACS4, LACS5, LACS6, LACS7, LACS8 and LACS9 [Shockey02]. Genetic and biochemical evidence have sorted the physiological roles of some of them.

LACS9 is the major chloroplast acyl-CoA synthetase [Schnurr02]. It contributes to the activation of de novo synthesized fatty acids during their export from the chloroplast. The enzyme is located on the chloroplast envelope. Knockout mutant plants contain only 10% of the chloroplast long-chain acyl-CoA synthetase activity. LACS9 is highly expressed in developing seeds and young rosette leaves.

Locations: plastid envelope

Unification Links: PhylomeDB:Q9CAP8 , Phytozome Plant Orthologs:AT1G77590.1 , Pride:Q9CAP8 , Protein Model Portal:Q9CAP8 , SMR:Q9CAP8 , String:3702.AT1G77590.1-P , TAIR:AT1G77590 , UniProt:Q9CAP8

Relationship Links: InterPro:IN-FAMILY:IPR000873 , InterPro:IN-FAMILY:IPR020845 , Pfam:IN-FAMILY:PF00501 , Prosite:IN-FAMILY:PS00455

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Instance reactions of [a long-chain fatty acid + ATP + coenzyme A → a long-chain acyl-CoA + AMP + diphosphate] (6.2.1.3):
i1: palmitate + ATP + coenzyme A → palmitoyl-CoA + AMP + diphosphate (6.2.1.3)

i2: stearate + ATP + coenzyme A = stearoyl-CoA + AMP + diphosphate (6.2.1.3)

i3: 18-hydroxyoleate + ATP + coenzyme A → 18-hydroxyoleoyl-CoA + AMP + diphosphate (6.2.1.3)

i4: 16-hydroxypalmitate + ATP + coenzyme A → 16-hydroxypalmitoyl-CoA + AMP + diphosphate (6.2.1.3)

i5: 18-hydroxystearate + ATP + coenzyme A → 18-hydroxystearoyl-CoA + AMP + diphosphate (6.2.1.3)

i6: an ω-hydroxy long-chain fatty acid + ATP + coenzyme A → an ω-hydroxy long-chain fatty acyl-CoA + AMP + diphosphate (6.2.1.-)

GO Terms:

Cellular Component: GO:0009526 - plastid envelope [Schnurr02]


Enzymatic reaction of: long-chain-fatty-acid-CoA ligase (long-chain acyl-CoA synthetase 9)

EC Number: 6.2.1.3

a long-chain fatty acid + ATP + coenzyme A <=> a long-chain acyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: suberin monomers biosynthesis , stearate biosynthesis II (bacteria and plants) , superpathway of fatty acid biosynthesis II (plant) , palmitate biosynthesis II (bacteria and plants) , long-chain fatty acid activation


Enzymatic reaction of: oleate:CoA ligase (long-chain acyl-CoA synthetase 9)

EC Number: 6.2.1.3

oleate + ATP + coenzyme A <=> oleoyl-CoA + AMP + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of fatty acid biosynthesis II (plant) , oleate biosynthesis I (plants)


References

Schnurr02: Schnurr JA, Shockey JM, de Boer GJ, Browse JA (2002). "Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis." Plant Physiol 129(4);1700-9. PMID: 12177483

Shockey02: Shockey JM, Fulda MS, Browse JA (2002). "Arabidopsis contains nine long-chain acyl-coenzyme a synthetase genes that participate in fatty acid and glycerolipid metabolism." Plant Physiol 129(4);1710-22. PMID: 12177484


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Sep 3, 2015, biocyc14.