Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
twitter

MetaCyc Enzyme: xyloglucan galactosyltransferase

Gene: KAM1 Accession Numbers: AT2G20370 (MetaCyc), At2g20370

Synonyms: MUR3

Species: Arabidopsis thaliana col

Summary:
Biochemical analysis using crude extracts from Komagataella pastoris cells over-expressing the Arabidopsis thaliana KAM1 protein using radiolabeled UDP-α-D-galactose as the donor substrate revealed that this xyloglucan galactosyltransferase attaches a D-galactose residue specifically to the third xylose residue within the XXXG core structure [Madson03].

The KAM1 gene product has also been implicated in endomembrane organization, and appears to be necessary for cell elongation [Tamura05a].

Locations: Golgi apparatus

Molecular Weight of Polypeptide: 70.744 kD (from nucleotide sequence)

Unification Links: ArrayExpress:Q7XJ98 , Entrez-gene:816556 , PhylomeDB:Q7XJ98 , Phytozome Plant Orthologs:At2g20370.1 , Pride:Q7XJ98 , String:Q7XJ98 , TAIR:At2g20370 , UniProt:Q7XJ98

Relationship Links: CAZy:IN-FAMILY:GT47 , InterPro:IN-FAMILY:IPR004263 , Panther:IN-FAMILY:PTHR11062 , Pfam:IN-FAMILY:PF03016

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005794 - Golgi apparatus [Chevalier10]

Credits:
Created 15-May-2008 by Karthikeyan AS , TAIR
Revised 01-Jun-2011 by Caspi R , SRI International


Enzymatic reaction of: xyloglucan galactosyltransferase

UDP-α-D-galactose + an XLXG xylogulcan <=> an XLLG xylogulcan + UDP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Enzymatic reaction of: xyloglucan galactosyltransferase

UDP-α-D-galactose + an XXXG xylogulcan <=> an XXLG xylogulcan + UDP

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: xyloglucan biosynthesis


References

Chevalier10: Chevalier L, Bernard S, Ramdani Y, Lamour R, Bardor M, Lerouge P, Follet-Gueye ML, Driouich A (2010). "Subcompartment localization of the side chain xyloglucan-synthesizing enzymes within Golgi stacks of tobacco suspension-cultured cells." Plant J 64(6);977-89. PMID: 21143678

Madson03: Madson M, Dunand C, Li X, Verma R, Vanzin GF, Caplan J, Shoue DA, Carpita NC, Reiter WD (2003). "The MUR3 gene of Arabidopsis encodes a xyloglucan galactosyltransferase that is evolutionarily related to animal exostosins." Plant Cell 15(7);1662-70. PMID: 12837954

Tamura05a: Tamura K, Shimada T, Kondo M, Nishimura M, Hara-Nishimura I (2005). "KATAMARI1/MURUS3 Is a novel golgi membrane protein that is required for endomembrane organization in Arabidopsis." Plant Cell 17(6);1764-76. PMID: 15863516


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc13.