MetaCyc Enzyme: glycerol-3-phosphate dehydrogenase (NAD-dependent)

Gene: At2G41540 Accession Number: AT2G41540 (MetaCyc)

Synonyms: GPDHc1

Species: Arabidopsis thaliana col

A glycerol-3-P dehydrogenase (GPDH) cDNA was cloned from Arabidopsis in a complementation screening using an Escherichia coli GPDH mutant. The cDNA was found to be encoded by At2G41540. T-DNA insertion mutants in this Arabidopsis gene (At2G41540, GPDHc1) was subsequently identified and characterized.

Total cellular NAD-dependent GPDH activity was found reduced in the mutants, whereas no reduction of the enzyme activity was detected in any other subcellular compartments, indicating a cytosolic localization of the enzyme.

Since the NAD-dependent GPDH reaction consumes NADH and regenerates NAD+, At2G41540 mutants, with the corresponding enzymatic activity blocked, exhibited increased NADH/NAD+ ratios compared with wild type plants, indicating a clear role of the NAD-dependent GPDH in maintaining cellular NADH/NAD+ homeostasis.

Locations: cytosol

Molecular Weight of Polypeptide: 51.5 kD (from nucleotide sequence)

Unification Links: TAIR:At2G41540

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005829 - cytosol [Shen06a]

Created 08-Mar-2010 by Zhang P , TAIR

Enzymatic reaction of: glycerol-3-phosphate dehydrogenase (NAD-dependent)

EC Number:

sn-glycerol 3-phosphate + NAD+ <=> glycerone phosphate + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: glycerol-3-phosphate shuttle


Shen06a: Shen W, Wei Y, Dauk M, Tan Y, Taylor DC, Selvaraj G, Zou J (2006). "Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis." Plant Cell 18(2);422-41. PMID: 16415206

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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