|Gene:||At2G41540||Accession Number: AT2G41540 (MetaCyc)|
Species: Arabidopsis thaliana col
A glycerol-3-P dehydrogenase (GPDH) cDNA was cloned from Arabidopsis in a complementation screening using an Escherichia coli GPDH mutant. The cDNA was found to be encoded by At2G41540. T-DNA insertion mutants in this Arabidopsis gene (At2G41540, GPDHc1) was subsequently identified and characterized.
Total cellular NAD-dependent GPDH activity was found reduced in the mutants, whereas no reduction of the enzyme activity was detected in any other subcellular compartments, indicating a cytosolic localization of the enzyme.
Since the NAD-dependent GPDH reaction consumes NADH and regenerates NAD+, At2G41540 mutants, with the corresponding enzymatic activity blocked, exhibited increased NADH/NAD+ ratios compared with wild type plants, indicating a clear role of the NAD-dependent GPDH in maintaining cellular NADH/NAD+ homeostasis.
Molecular Weight of Polypeptide: 51.5 kD (from nucleotide sequence)
Unification Links: TAIR:At2G41540
|Cellular Component:||GO:0005829 - cytosol [Shen06]|
Enzymatic reaction of: glycerol-3-phosphate dehydrogenase (NAD-dependent)
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
This reaction is reversible.
In Pathways: glycerol-3-phosphate shuttle
Shen06: Shen W, Wei Y, Dauk M, Tan Y, Taylor DC, Selvaraj G, Zou J (2006). "Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis." Plant Cell 18(2);422-41. PMID: 16415206
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