Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: glycerol-3-phosphate dehydrogenase (NAD-dependent)

Gene: At2G41540 Accession Number: AT2G41540 (MetaCyc)

Synonyms: GPDHc1

Species: Arabidopsis thaliana col

Summary:
A glycerol-3-P dehydrogenase (GPDH) cDNA was cloned from Arabidopsis in a complementation screening using an Escherichia coli GPDH mutant. The cDNA was found to be encoded by At2G41540. T-DNA insertion mutants in this Arabidopsis gene (At2G41540, GPDHc1) was subsequently identified and characterized.

Total cellular NAD-dependent GPDH activity was found reduced in the mutants, whereas no reduction of the enzyme activity was detected in any other subcellular compartments, indicating a cytosolic localization of the enzyme.

Since the NAD-dependent GPDH reaction consumes NADH and regenerates NAD+, At2G41540 mutants, with the corresponding enzymatic activity blocked, exhibited increased NADH/NAD+ ratios compared with wild type plants, indicating a clear role of the NAD-dependent GPDH in maintaining cellular NADH/NAD+ homeostasis.

Locations: cytosol

Molecular Weight of Polypeptide: 51.5 kD (from nucleotide sequence)

Unification Links: TAIR:At2G41540

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005829 - cytosol [Shen06a]

Credits:
Created 08-Mar-2010 by Zhang P , TAIR


Enzymatic reaction of: glycerol-3-phosphate dehydrogenase (NAD-dependent)

EC Number: 1.1.1.8

sn-glycerol 3-phosphate + NAD+ <=> dihydroxyacetone phosphate + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: glycerol-3-phosphate shuttle


References

Shen06a: Shen W, Wei Y, Dauk M, Tan Y, Taylor DC, Selvaraj G, Zou J (2006). "Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis." Plant Cell 18(2);422-41. PMID: 16415206


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, biocyc12.