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discounted EARLY registration ends Dec 31, 2014
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites MAYBE down
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MetaCyc Enzyme: CYP83A1 monooxygenase

Gene: At4g13770 Accession Number: AT4G13770 (MetaCyc)

Synonyms: CYP83A1

Species: Arabidopsis thaliana col

Subunit composition of CYP83A1 monooxygenase = [At4g13770]
         CYP83A1 = At4g13770

Summary:
CYP83A1 metabolizes aldoximes derived from chain-elongated methionine. The Km for these aliphatic aldoximes is in the range of 20 to 150 uM [Naur03].

Both CYP83A1 and CYP83B1 metabolize phenylacetaldoxime. However phenylacetaldoxime is a better substrate for CYP83B1. The Km of CYP83A1 for phenylacetaldoxime is 3-fold higher than that of CYP83B1. The catalytic efficiency (Kcat/Km) of CYP83B1 is 6 times higher than that of CYP83A1 [Naur03].

The reaction is thought to involve a carbon-sulfur lyase to form the thiohydroximate from the intermediate alkylthiohydroximate [Wittstock02].

Unification Links: ArrayExpress:P48421 , PhylomeDB:P48421 , Pride:P48421 , Protein Model Portal:P48421 , SMR:P48421 , String:P48421 , TAIR:AT4G13770 , UniProt:P48421

Relationship Links: InterPro:IN-FAMILY:IPR001128 , InterPro:IN-FAMILY:IPR002401 , InterPro:IN-FAMILY:IPR017972 , Pfam:IN-FAMILY:PF00067 , Prints:IN-FAMILY:PR00385 , Prints:IN-FAMILY:PR00463 , Prosite:IN-FAMILY:PS00086

Gene-Reaction Schematic: ?


Enzymatic reaction of: CYP83A1 9-methylthiononanaldoxime monooxygenase (CYP83A1 monooxygenase)

9-methylthiononanaldoxime + NADP+ <=> 9-methylthiononanonitrile oxide + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate biosynthesis from hexahomomethionine


Enzymatic reaction of: CYP83A1 8-methylthiooctanaldoxime monooxygenase (CYP83A1 monooxygenase)

8-methylthiooctanaldoxime + NADP+ <=> 8-methylthiooctanonitrile oxide + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate biosynthesis from pentahomomethionine


Enzymatic reaction of: CYP83A1 7-methylthioheptanaldoxime monooxygenase (CYP83A1 monooxygenase)

7-methylthioheptanaldoxime + NADP+ <=> 7-methylthioheptanonitrile oxide + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate biosynthesis from tetrahomomethionine


Enzymatic reaction of: CYP83A1 6-methylthiohexanaldoxime monooxygenase (CYP83A1 monooxygenase)

6-methylthiohexanaldoxime + NADP+ <=> 6-methylthiohexanonitrile oxide + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate biosynthesis from trihomomethionine


Enzymatic reaction of: CYP83A1 5-methylthiopentanaldoxime monooxygenase (CYP83A1 monooxygenase)

5-methylthiopentanaldoxime + NADP+ <=> 5-methylthiopentanonitrile oxide + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate biosynthesis from dihomomethionine


Enzymatic reaction of: CYP83A1 4-methylthiobutanaldoxime monooxygenase (CYP83A1 monooxygenase)

4-methylthiobutanaldoxime + NADP+ <=> 4-methylthiobutanonitrile oxide + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate biosynthesis from homomethionine


Enzymatic reaction of: CYP83A1 phenylacetaldoxime monooxygenase (CYP83A1 monooxygenase)

EC Number: 1.-.-.-

(Z)-phenylacetaldehyde oxime + NADP+ <=> phenylacetonitrile oxide + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: glucosinolate biosynthesis from phenylalanine

Kinetic Parameters:

Substrate
Km (μM)
Citations
(Z)-phenylacetaldehyde oxime
556.0
[Naur03]


References

Naur03: Naur P, Petersen BL, Mikkelsen MD, Bak S, Rasmussen H, Olsen CE, Halkier BA (2003). "CYP83A1 and CYP83B1, Two Nonredundant Cytochrome P450 Enzymes Metabolizing Oximes in the Biosynthesis of Glucosinolates in Arabidopsis." Plant Physiol 133(1);63-72. PMID: 12970475

Wittstock02: Wittstock U, Halkier BA (2002). "Glucosinolate research in the Arabidopsis era." Trends Plant Sci 2002;7(6);263-70. PMID: 12049923


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 25, 2014, biocyc12.