MetaCyc Polypeptide: hydrogenase 1, b-type cytochrome subunit

Gene: hyaC Accession Numbers: EG10470 (MetaCyc), b0974, ECK0965

Synonyms: cybH

Species: Escherichia coli K-12 substr. MG1655

Component of: hydrogenase 1 (extended summary available)

The hyaC gene product is very hydrophobic, rich in aromatic residues, and has four putative hydrophobic membrane-spanning regions [Menon90].

An in-frame deletion in the hyaC gene results in wild-type levels of hydrogenase 1 activity, although resulting in the appearance of multiple forms of the enzyme [Menon91].

Review: [Vignais04]

Locations: inner membrane

Map Position: [1,034,289 -> 1,034,996]

Molecular Weight of Polypeptide: 27.597 kD (from nucleotide sequence)

pI: 9.42

Unification Links: ASAP:ABE-0003290 , CGSC:31788 , DIP:DIP-35859N , EchoBASE:EB0465 , EcoGene:EG10470 , EcoliWiki:b0974 , ModBase:P0AAM1 , OU-Microarray:b0974 , PortEco:hyaC , Protein Model Portal:P0AAM1 , RefSeq:NP_415493 , RegulonDB:EG10470 , SMR:P0AAM1 , String:511145.b0974 , UniProt:P0AAM1

Relationship Links: InterPro:IN-FAMILY:IPR000516 , InterPro:IN-FAMILY:IPR011577 , InterPro:IN-FAMILY:IPR016174 , PDB:Structure:4GD3 , Pfam:IN-FAMILY:PF00033 , Pfam:IN-FAMILY:PF01292 , Prints:IN-FAMILY:PR00161 , Prosite:IN-FAMILY:PS00882 , Prosite:IN-FAMILY:PS00883

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [GOA01a]
GO:0009060 - aerobic respiration
GO:0009061 - anaerobic respiration
GO:0022904 - respiratory electron transport chain Inferred by computational analysis [GOA01a]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005506 - iron ion binding Inferred by computational analysis [GOA01a]
GO:0009055 - electron carrier activity Inferred by computational analysis [GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of macromolecules (cellular constituents) large molecule carriers cytochromes
metabolism energy metabolism, carbon aerobic respiration
metabolism energy metabolism, carbon anaerobic respiration
metabolism energy production/transport electron donors

Imported from EcoCyc 27-Jan-2015 by Paley S , SRI International

Subunit of: hydrogenase 1

Synonyms: HYD1, hydrogenase I, NiFe hydrogenase

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of hydrogenase 1 = [HyaA][HyaB][HyaC]
         hydrogenase 1, small subunit = HyaA (summary available)
         hydrogenase 1, large subunit = HyaB (summary available)
         hydrogenase 1, b-type cytochrome subunit = HyaC (summary available)

Hydrogenase 1 mediates hydrogen uptake in the presence of high-potential acceptors such as ferricyanide and phenazine methosulfate, but not low-potential acceptors [Laurinavichene01]. The enzyme is more tolerant to oxygen than hydrogenase 2, providing complementary redox properties to the cell [Laurinavichene02, Lukey10, Wulff14].

Hydrogenase 1 contains a [3Fe-4S] or [4Fe-4S] cluster (depending on oxidation state) and nickel [DerVartanian96]. The substrate specificity of hydrogenase 1 for various quinones is unknown [Laurinavichene01].

HybG [Blokesch01] and HybF [Hube02] are involved in maturation of hydrogenase 1.

E. coli K-12 contains a second respiratory hydrogenase - hydrogenase 2 - and a third hydrogenase - hydrogenase 3 - which is part of the formate hydrogenlyase complex. A potential fourth hydrogenase - hydrogenase 4 - is encoded within the hyf operon. Only hydrogenase 1 can reduce nitroblue tetrazolium in anaerobically growing E. coli cells [Pinske12b].

Review: [Vignais04]

Citations: [Pinske11]

Locations: membrane

GO Terms:

Molecular Function: GO:0016151 - nickel cation binding Inferred from experiment [DerVartanian96]
GO:0051536 - iron-sulfur cluster binding Inferred from experiment [DerVartanian96]
Cellular Component: GO:0016020 - membrane [Ballantine85]

Imported from EcoCyc 27-Jan-2015 by Paley S , SRI International

Enzymatic reaction of: hydrogenase

Synonyms: hydrogenlyase

Transport reaction diagram for hydrogenase

Imported from EcoCyc 27-Jan-2015 by Paley S , SRI International

There are four hydrogenases in E. coli which are synthesized in response to different physiological conditions. Hydrogenase 1 is a membrane-bound nickel-containing protein [Menon90, Sawers94]. It contains as electron-transfer components Fe-S clusters and nickel. It catalyzes the oxidation of hydrogen; the physiological electron carrier is unknown. The enzyme effects proton translocation coupled to the scalar redox reaction. [Sawers86]

Cofactors or Prosthetic Groups: [FeS] iron-sulfur cluster [DerVartanian96], Ni2+ [Comment 1, DerVartanian96, Sawers86]

Inhibitors (Unknown Mechanism): carbon monoxide [Sawers86] , azide [Sawers86] , N-bromosuccinimide [Sawers86]

Kinetic Parameters:

Km (μM)

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 20 -> 40
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 64 -> 84
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 131 -> 151
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 186 -> 203
UniProt: Helical;; Non-Experimental Qualifier: potential;

10/20/97 Gene b0974 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10470; confirmed by SwissProt match.


Ballantine85: Ballantine SP, Boxer DH (1985). "Nickel-containing hydrogenase isoenzymes from anaerobically grown Escherichia coli K-12." J Bacteriol 163(2);454-9. PMID: 3894325

Blokesch01: Blokesch M, Magalon A, Bock A (2001). "Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli." J Bacteriol 183(9);2817-22. PMID: 11292801

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DerVartanian96: DerVartanian ME, Menon NK, Przybyla AE, Peck HD, DerVartanian DV (1996). "Electron paramagnetic resonance (EPR) studies on hydrogenase-1 (HYD1) purified from a mutant strain (AP6) of Escherichia coli enhanced in HYD1." Biochem Biophys Res Commun 227(1);211-5. PMID: 8858127

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hube02: Hube M, Blokesch M, Bock A (2002). "Network of hydrogenase maturation in Escherichia coli: role of accessory proteins HypA and HybF." J Bacteriol 184(14);3879-85. PMID: 12081959

Laurinavichene01: Laurinavichene TV, Tsygankov AA (2001). "H2 consumption by Escherichia coli coupled via hydrogenase 1 or hydrogenase 2 to different terminal electron acceptors." FEMS Microbiol Lett 202(1);121-4. PMID: 11506918

Laurinavichene02: Laurinavichene TV, Zorin NA, Tsygankov AA (2002). "Effect of redox potential on activity of hydrogenase 1 and hydrogenase 2 in Escherichia coli." Arch Microbiol 178(6);437-42. PMID: 12420163

Lukey10: Lukey MJ, Parkin A, Roessler MM, Murphy BJ, Harmer J, Palmer T, Sargent F, Armstrong FA (2010). "How Escherichia coli is equipped to oxidize hydrogen under different redox conditions." J Biol Chem 285(6);3928-38. PMID: 19917611

Menon90: Menon NK, Robbins J, Peck HD, Chatelus CY, Choi ES, Przybyla AE (1990). "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames." J Bacteriol 1990;172(4);1969-77. PMID: 2180913

Menon91: Menon NK, Robbins J, Wendt JC, Shanmugam KT, Przybyla AE (1991). "Mutational analysis and characterization of the Escherichia coli hya operon, which encodes [NiFe] hydrogenase 1." J Bacteriol 173(15);4851-61. PMID: 1856178

Pinske11: Pinske C, Sawers G (2011). "Iron restriction induces preferential down-regulation of H(2)-consuming over H(2)-evolving reactions during fermentative growth of Escherichia coli." BMC Microbiol 11;196. PMID: 21880124

Pinske12b: Pinske C, Jaroschinsky M, Sargent F, Sawers G (2012). "Zymographic differentiation of [NiFe]-hydrogenases 1, 2 and 3 of Escherichia coli K-12." BMC Microbiol 12;134. PMID: 22769583

Sawers86: Sawers RG, Boxer DH (1986). "Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12." Eur J Biochem 1986;156(2);265-75. PMID: 3516689

Sawers94: Sawers G (1994). "The hydrogenases and formate dehydrogenases of Escherichia coli." Antonie Van Leeuwenhoek 1994;66(1-3);57-88. PMID: 7747941

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Vignais04: Vignais PM, Colbeau A (2004). "Molecular biology of microbial hydrogenases." Curr Issues Mol Biol 6(2);159-88. PMID: 15119826

Wulff14: Wulff P, Day CC, Sargent F, Armstrong FA (2014). "How oxygen reacts with oxygen-tolerant respiratory [NiFe]-hydrogenases." Proc Natl Acad Sci U S A 111(18);6606-11. PMID: 24715724

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon May 25, 2015, BIOCYC14A.