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MetaCyc Enzyme: dTDP-4-dehydro-6-deoxy-D-glucose transaminase

Gene: rffA Accession Numbers: EG11456 (MetaCyc), b3791, ECK3783

Synonyms: rff, fcnA, wecE, yifI

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of dTDP-4-dehydro-6-deoxy-D-glucose transaminase = [RffA]4
         dTDP-4-dehydro-6-deoxy-D-glucose transaminase = RffA

Summary:
dTDP-4-dehydro-6-deoxy-D-glucose transaminase is involved in the biosynthesis of enterobacterial common antigen (ECA). This enzyme catalyzes the conversion of TDP-4-keto-6-deoxy-D-glucose to TDP-D-fucosamine. [MeierDieter90, Hwang04].

A Tn10 insertion mutant does not produce ECA [MeierDieter90].

Citations: [Danese98a, Barr93, MeierDieter92]

Locations: cytosol

Map Position: [3,973,169 -> 3,974,299]

Molecular Weight of Polypeptide: 41.901 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012384 , EchoBASE:EB1425 , EcoGene:EG11456 , EcoliWiki:b3791 , ModBase:P27833 , OU-Microarray:b3791 , PortEco:rffA , PR:PRO_000023732 , Pride:P27833 , Protein Model Portal:P27833 , RefSeq:NP_418238 , RegulonDB:EG11456 , SMR:P27833 , String:511145.b3791 , UniProt:P27833

Relationship Links: InterPro:IN-FAMILY:IPR000653 , InterPro:IN-FAMILY:IPR012749 , InterPro:IN-FAMILY:IPR015421 , InterPro:IN-FAMILY:IPR015422 , InterPro:IN-FAMILY:IPR015424 , Pfam:IN-FAMILY:PF01041

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009246 - enterobacterial common antigen biosynthetic process Inferred from experiment [MeierDieter90]
GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [UniProtGOA11]
GO:0009243 - O antigen biosynthetic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0019180 - dTDP-4-amino-4,6-dideoxygalactose transaminase activity Inferred from experiment Inferred by computational analysis [GOA01a, Hwang04]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0030170 - pyridoxal phosphate binding Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell structure surface antigens (ECA, O antigen of LPS)
metabolism biosynthesis of macromolecules (cellular constituents) enterobacterial common antigen (surface glycolipid)

Credits:
Created in EcoCyc 03-Dec-2012 by Keseler I , SRI International
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: dTDP-4-dehydro-6-deoxy-D-glucose transaminase

Synonyms: TDP-4-oxo-6-deoxy-D-glucose transaminase, dTDP-4-oxo-6-deoxy-D-glucose transaminase

EC Number: 2.6.1.59

dTDP-thomosamine + 2-oxoglutarate <=> dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + L-glutamate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

Alternative Substrates for L-glutamate: L-glutamine [Hwang04 ]

In Pathways: superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis , enterobacterial common antigen biosynthesis , dTDP-N-acetylthomosamine biosynthesis

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Cofactors or Prosthetic Groups: pyridoxal 5'-phosphate [Hwang04]

Kinetic Parameters:

Substrate
Km (μM)
kcat (sec-1)
kcat/Km (sec-1 μM-1)
Citations
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose
110.0
0.38
[Hwang04]

T(opt): 37 °C [BRENDA14, Hwang04]

pH(opt): 7.5 [Hwang04]


Sequence Features

Feature Class Location Citations Comment
N6-pyridoxal-phosphate-Lys-Modification 181
[UniProt11a]
UniProt: N6-(pyridoxal phosphate)lysine; Non-Experimental Qualifier: by similarity.

History:
7/13/1999 (pkarp) Merged genes G349/rffA and EG11456/wecE
10/20/97 Gene b3791 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11456.


References

Barr93: Barr K, Rick PD (1993). "Physical map location of the rffC and rffA genes of Escherichia coli." J Bacteriol 175(17);5738-9. PMID: 8366065

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Danese98a: Danese PN, Oliver GR, Barr K, Bowman GD, Rick PD, Silhavy TJ (1998). "Accumulation of the enterobacterial common antigen lipid II biosynthetic intermediate stimulates degP transcription in Escherichia coli." J Bacteriol 1998;180(22);5875-84. PMID: 9811644

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hwang04: Hwang BY, Lee HJ, Yang YH, Joo HS, Kim BG (2004). "Characterization and investigation of substrate specificity of the sugar aminotransferase WecE from E. coli K12." Chem Biol 11(7);915-25. PMID: 15271350

MeierDieter90: Meier-Dieter U, Starman R, Barr K, Mayer H, Rick PD (1990). "Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis." J Biol Chem 1990;265(23);13490-7. PMID: 2166030

MeierDieter92: Meier-Dieter U, Barr K, Starman R, Hatch L, Rick PD (1992). "Nucleotide sequence of the Escherichia coli rfe gene involved in the synthesis of enterobacterial common antigen. Molecular cloning of the rfe-rff gene cluster." J Biol Chem 1992;267(2);746-53. PMID: 1730666

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC14B.