|Gene:||rhaM||Accession Numbers: EG11865 (MetaCyc), b3901, ECK3894|
Species: Escherichia coli K-12 substr. MG1655
A crystal structure of RhaM in complex with L-rhamnose was solved at 1.8 Å resolution. Analysis of the catalytic activity of mutants in predicted active site residues allowed the authors to propose a possible catalytic mechanism [Ryu05a].
A rhaM deletion mutant has a decreased growth rate when grown on low concentrations of L-rhamnose [Ryu05a].
|Map Position: [4,091,147 <- 4,091,461]|
Molecular Weight of Polypeptide: 12.265 kD (from nucleotide sequence)
Molecular Weight of Multimer: 23.7 kD (experimental) [Ryu05a]
Unification Links: ASAP:ABE-0012729 , EchoBASE:EB1811 , EcoGene:EG11865 , EcoliWiki:b3901 , OU-Microarray:b3901 , PortEco:rhaM , PR:PRO_000023736 , Protein Model Portal:P32156 , RefSeq:NP_418337 , RegulonDB:EG11865 , SMR:P32156 , String:511145.b3901 , UniProt:P32156
|Biological Process:||GO:0019301 - rhamnose catabolic process
GO:0005975 - carbohydrate metabolic process [UniProtGOA11a]
GO:0019299 - rhamnose metabolic process [UniProtGOA12, UniProtGOA11a, GOA06, GOA01a]
|Molecular Function:||GO:0016857 - racemase and epimerase activity, acting on carbohydrates and derivatives
[GOA01, GOA01a, Ryu04]
GO:0042803 - protein homodimerization activity [Ryu05a]
GO:0016853 - isomerase activity [UniProtGOA11a, GOA06]
|Cellular Component:||GO:0005737 - cytoplasm [UniProtGOA11, UniProtGOA11a, GOA06, GOA01a]|
|MultiFun Terms:||metabolism → carbon utilization|
Enzymatic reaction of: L-rhamnose mutarotase
EC Number: 5.1.3.j
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is favored in the direction shown.
|Protein-Segment||76 -> 77|
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b3901 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11865; confirmed by SwissProt match.
Ryu04: Ryu KS, Kim C, Kim I, Yoo S, Choi BS, Park C (2004). "NMR application probes a novel and ubiquitous family of enzymes that alter monosaccharide configuration." J Biol Chem 279(24);25544-8. PMID: 15060078
Ryu05a: Ryu KS, Kim JI, Cho SJ, Park D, Park C, Cheong HK, Lee JO, Choi BS (2005). "Structural insights into the monosaccharide specificity of Escherichia coli rhamnose mutarotase." J Mol Biol 349(1);153-62. PMID: 15876375
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493