MetaCyc Enzyme: L-rhamnose mutarotase

Gene: rhaM Accession Numbers: EG11865 (MetaCyc), b3901, ECK3894

Synonyms: yiiL

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of L-rhamnose mutarotase = [RhaM]2
         L-rhamnose mutarotase = RhaM

Utilizing NMR techniques, RhaM was shown to catalyze the anomeric conversion of rhamnose [Ryu04]. The enzyme has a preference for binding the β-anomer [Ryu05].

A crystal structure of RhaM in complex with L-rhamnose was solved at 1.8 Å resolution. Analysis of the catalytic activity of mutants in predicted active site residues allowed the authors to propose a possible catalytic mechanism [Ryu05].

A rhaM deletion mutant has a decreased growth rate when grown on low concentrations of L-rhamnose [Ryu05].

Locations: cytosol

Map Position: [4,091,147 <- 4,091,461]

Molecular Weight of Polypeptide: 12.265 kD (from nucleotide sequence)

Molecular Weight of Multimer: 23.7 kD (experimental) [Ryu05]

Unification Links: ASAP:ABE-0012729 , EchoBASE:EB1811 , EcoGene:EG11865 , EcoliWiki:b3901 , OU-Microarray:b3901 , PortEco:rhaM , PR:PRO_000023736 , Protein Model Portal:P32156 , RefSeq:NP_418337 , RegulonDB:EG11865 , SMR:P32156 , String:511145.b3901 , UniProt:P32156

Relationship Links: InterPro:IN-FAMILY:IPR008000 , InterPro:IN-FAMILY:IPR011008 , InterPro:IN-FAMILY:IPR013448 , PDB:Structure:1X8D , Pfam:IN-FAMILY:PF05336

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0019301 - rhamnose catabolic process Inferred from experiment [Ryu05]
GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [UniProtGOA11]
GO:0019299 - rhamnose metabolic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11, GOA06, GOA01]
Molecular Function: GO:0016857 - racemase and epimerase activity, acting on carbohydrates and derivatives Inferred from experiment Inferred by computational analysis [GOA01, Ryu04]
GO:0042803 - protein homodimerization activity Inferred from experiment [Ryu05]
GO:0016853 - isomerase activity Inferred by computational analysis [UniProtGOA11, GOA06]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, GOA01]

MultiFun Terms: metabolism carbon utilization

Created in EcoCyc 06-Nov-2007 by Keseler I , SRI International
Imported from EcoCyc 02-Jun-2015 by Paley S , SRI International

Enzymatic reaction of: L-rhamnose mutarotase

EC Number:

α-L-rhamnopyranose <=> β-L-rhamnopyranose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of fucose and rhamnose degradation , L-rhamnose degradation I

Imported from EcoCyc 02-Jun-2015 by Paley S , SRI International

Sequence Features

Feature Class Location Citations Comment
Amino-Acid-Sites-That-Bind 18
UniProt: Substrate.
Mutagenesis-Variant 18
[Ryu05, UniProt11]
UniProt: Loss of mutarotase activity.
Active-Site 22
UniProt: Proton donor; Non-Experimental Qualifier: probably;
Amino-Acid-Sites-That-Bind 41
UniProt: Substrate.
Protein-Segment 76 -> 77
UniProt: Substrate binding; Sequence Annotation Type: region of interest;

Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b3901 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11865; confirmed by SwissProt match.


GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Ryu04: Ryu KS, Kim C, Kim I, Yoo S, Choi BS, Park C (2004). "NMR application probes a novel and ubiquitous family of enzymes that alter monosaccharide configuration." J Biol Chem 279(24);25544-8. PMID: 15060078

Ryu05: Ryu KS, Kim JI, Cho SJ, Park D, Park C, Cheong HK, Lee JO, Choi BS (2005). "Structural insights into the monosaccharide specificity of Escherichia coli rhamnose mutarotase." J Mol Biol 349(1);153-62. PMID: 15876375

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, biocyc12.