Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: L-rhamnose mutarotase

Gene: rhaM Accession Numbers: EG11865 (MetaCyc), b3901, ECK3894

Synonyms: yiiL

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of L-rhamnose mutarotase = [RhaM]2
         L-rhamnose mutarotase = RhaM

Summary:
Utilizing NMR techniques, RhaM was shown to catalyze the anomeric conversion of rhamnose [Ryu04]. The enzyme has a preference for binding the β-anomer [Ryu05a].

A crystal structure of RhaM in complex with L-rhamnose was solved at 1.8 Å resolution. Analysis of the catalytic activity of mutants in predicted active site residues allowed the authors to propose a possible catalytic mechanism [Ryu05a].

A rhaM deletion mutant has a decreased growth rate when grown on low concentrations of L-rhamnose [Ryu05a].

Locations: cytosol

Map Position: [4,091,147 <- 4,091,461]

Molecular Weight of Polypeptide: 12.265 kD (from nucleotide sequence)

Molecular Weight of Multimer: 23.7 kD (experimental) [Ryu05a]

Unification Links: ASAP:ABE-0012729 , EchoBASE:EB1811 , EcoGene:EG11865 , EcoliWiki:b3901 , OU-Microarray:b3901 , PortEco:rhaM , PR:PRO_000023736 , Protein Model Portal:P32156 , RefSeq:NP_418337 , RegulonDB:EG11865 , SMR:P32156 , String:511145.b3901 , UniProt:P32156

Relationship Links: InterPro:IN-FAMILY:IPR008000 , InterPro:IN-FAMILY:IPR011008 , InterPro:IN-FAMILY:IPR013448 , PDB:Structure:1X8D , Pfam:IN-FAMILY:PF05336

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0019301 - rhamnose catabolic process Inferred from experiment [Ryu05a]
GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0019299 - rhamnose metabolic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11a, GOA06, GOA01a]
Molecular Function: GO:0016857 - racemase and epimerase activity, acting on carbohydrates and derivatives Inferred from experiment Inferred by computational analysis [GOA01, GOA01a, Ryu04]
GO:0042803 - protein homodimerization activity Inferred from experiment [Ryu05a]
GO:0016853 - isomerase activity Inferred by computational analysis [UniProtGOA11a, GOA06]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06, GOA01a]

MultiFun Terms: metabolism carbon utilization

Credits:
Created in EcoCyc 06-Nov-2007 by Keseler I , SRI International
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: L-rhamnose mutarotase

EC Number: 5.1.3.j

α-L-rhamnopyranose <=> β-L-rhamnopyranose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of fucose and rhamnose degradation , L-rhamnose degradation I

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Sequence Features

Feature Class Location Citations Comment
Mutagenesis-Variant 18
[Ryu05a, UniProt11a]
Alternate sequence: F; UniProt: Loss of mutarotase activity.
Amino-Acid-Sites-That-Bind 18
[UniProt10a]
UniProt: Substrate;
Active-Site 22
[UniProt10]
UniProt: Proton donor; Non-Experimental Qualifier: probably;
Amino-Acid-Sites-That-Bind 41
[UniProt10a]
UniProt: Substrate;
Protein-Segment 76 -> 77
[UniProt10]
UniProt: Substrate binding; Sequence Annotation Type: region of interest;

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b3901 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11865; confirmed by SwissProt match.


References

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Ryu04: Ryu KS, Kim C, Kim I, Yoo S, Choi BS, Park C (2004). "NMR application probes a novel and ubiquitous family of enzymes that alter monosaccharide configuration." J Biol Chem 279(24);25544-8. PMID: 15060078

Ryu05a: Ryu KS, Kim JI, Cho SJ, Park D, Park C, Cheong HK, Lee JO, Choi BS (2005). "Structural insights into the monosaccharide specificity of Escherichia coli rhamnose mutarotase." J Mol Biol 349(1);153-62. PMID: 15876375

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13A.