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MetaCyc Enzyme: glucosamine-6-phosphate N-acetyltransferase 1

Gene: Gnpnat1 Accession Number: G-10022 (MetaCyc)

Synonyms: EMeg32

Species: Mus musculus

Summary:
Recombinant mouse glucosamine-6-phosphate acetyltransferase 1 has been expressed in both Escherichia coli and insect cells. The subunit relative molecular mass was estimated by SDS-PAGE, but the subunit structure was not determined [Boehmelt00].

Glucosamine-6-phosphate N-acetyltransferase is a member of the Gcn5-related N-acetyltransferases that catalyze the transfer of an acetyl group of acetyl-CoA to the primary amine of an acceptor substrate. The crystal structure of the enzyme from Saccharomyces cerevisiae has been determined and indicated a homodimeric structure [Peneff01]. Reviewed in [Milewski06].

Locations: late endosome membrane, Golgi membrane, lysosomal membrane

Map Position: [44,298,298 <- 44,310,673]

Molecular Weight of Polypeptide: 20.791 kD (from nucleotide sequence), 21 kD (experimental) [Boehmelt00 ]

Unification Links: Entrez-gene:54342 , Mint:MINT-1605392 , PhosphoSite:Q9JK38 , Pride:Q9JK38 , Protein Model Portal:Q9JK38 , SMR:Q9JK38 , String:Q9JK38 , UniProt:Q9JK38

Relationship Links: InterPro:IN-FAMILY:IPR000182 , InterPro:IN-FAMILY:IPR016181 , Pfam:IN-FAMILY:PF13508 , Prosite:IN-FAMILY:PS51186

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0000139 - Golgi membrane [Boehmelt00]
GO:0005765 - lysosomal membrane [Boehmelt00]
GO:0031902 - late endosome membrane [Boehmelt00]

Credits:
Created 20-Apr-2007 by Fulcher CA , SRI International


Enzymatic reaction of: glucosamine-6-phosphate acetyltransferase (glucosamine-6-phosphate N-acetyltransferase 1)

Synonyms: glucosamine-6-phosphate N-acetyltransferase, phosphoglucosamine acetyltransferase, acetyl-CoA:D-glucosamine-6-phosphate N-acetyltransferase, EMeg32/mGlcN6P-AT

EC Number: 2.3.1.4

D-glucosamine 6-phosphate + acetyl-CoA <=> N-acetyl-D-glucosamine 6-phosphate + coenzyme A + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: UDP-N-acetyl-D-glucosamine biosynthesis II

Summary:
Kinetic parameters for murine glucosamine-6-phosphate acetyltransferase were determined for the recombinant, his-tagged enzyme expressed in insect cells using a baculovirus vector [Boehmelt00].

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-glucosamine 6-phosphate
40.0
[Boehmelt00]
acetyl-CoA
120.0
[Boehmelt00]


References

Boehmelt00: Boehmelt G, Fialka I, Brothers G, McGinley MD, Patterson SD, Mo R, Hui CC, Chung S, Huber LA, Mak TW, Iscove NN (2000). "Cloning and characterization of the murine glucosamine-6-phosphate acetyltransferase EMeg32. Differential expression and intracellular membrane association." J Biol Chem 275(17);12821-32. PMID: 10777580

Milewski06: Milewski S, Gabriel I, Olchowy J (2006). "Enzymes of UDP-GlcNAc biosynthesis in yeast." Yeast 23(1);1-14. PMID: 16408321

Peneff01: Peneff C, Mengin-Lecreulx D, Bourne Y (2001). "The crystal structures of Apo and complexed Saccharomyces cerevisiae GNA1 shed light on the catalytic mechanism of an amino-sugar N-acetyltransferase." J Biol Chem 276(19);16328-34. PMID: 11278591


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Wed Aug 5, 2015, biocyc11.