MetaCyc Enzyme: vanillin dehydrogenase

Gene: ligV Accession Number: G-11556 (MetaCyc)

Species: Sphingomonas sp. SYK6

The ligV gene of Sphingomonas sp. SYK6, encoding vanillin dehydrogenase, was identified by functional complementation of the vanillin-degradation defficient strain S. paucimobilis IAM 12578. The gene was cloned and expressed in Escherichia coli and cell extracts were assayed [Masai07].

Activity with NAD+ was approximately 90 times higher than that achieved with NADP. LigV has a wide substrate range. It exhibited the highest dehydrogenase activity toward vanillin, but showed relatively high levels of activity toward benzaldehyde, 4-hydroxybenzaldehyde, protocatechualdehyde, and 3-methoxybenzaldehyde.

A ligV mutant of Sphingomonas sp. SYK6 abolished normal growth on vanillin [Masai07].

Molecular Weight of Polypeptide: 50.359 kD (from nucleotide sequence), 50.0 kD (experimental) [Masai07 ]

Unification Links: Protein Model Portal:A2PZP3 , UniProt:A2PZP3

Relationship Links: Entrez-Nucleotide:PART-OF:AB287332 , InterPro:IN-FAMILY:IPR015590 , InterPro:IN-FAMILY:IPR016161 , InterPro:IN-FAMILY:IPR016162 , InterPro:IN-FAMILY:IPR016163 , InterPro:IN-FAMILY:IPR029510 , Pfam:IN-FAMILY:PF00171 , Prosite:IN-FAMILY:PS00687

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 25-Sep-2009 by Caspi R , SRI International

Enzymatic reaction of: vanillin dehydrogenase

EC Number:

vanillin + NAD+ + H2O <=> vanillate + NADH + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for vanillin: benzaldehyde [Masai07 ] , 4-hydroxybenzaldehyde [Masai07 ] , protocatechualdehyde [Masai07 ] , 3-methoxybenzaldehyde [Masai07 ]

In Pathways: superpathway of vanillin and vanillate degradation , vanillin and vanillate degradation I


Masai07: Masai E, Yamamoto Y, Inoue T, Takamura K, Hara H, Kasai D, Katayama Y, Fukuda M (2007). "Characterization of ligV essential for catabolism of vanillin by Sphingomonas paucimobilis SYK-6." Biosci Biotechnol Biochem 71(10);2487-92. PMID: 17928721

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc13.