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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: vanillin dehydrogenase

Gene: ligV Accession Number: G-11556 (MetaCyc)

Species: Sphingomonas sp. SYK6

Summary:
The ligV gene of Sphingomonas sp. SYK6, encoding vanillin dehydrogenase, was identified by functional complementation of the vanillin-degradation defficient strain S. paucimobilis IAM 12578. The gene was cloned and expressed in Escherichia coli and cell extracts were assayed [Masai07].

Activity with NAD+ was approximately 90 times higher than that achieved with NADP. LigV has a wide substrate range. It exhibited the highest dehydrogenase activity toward vanillin, but showed relatively high levels of activity toward benzaldehyde, 4-hydroxybenzaldehyde, protocatechualdehyde, and 3-methoxybenzaldehyde.

A ligV mutant of Sphingomonas sp. SYK6 abolished normal growth on vanillin [Masai07].

Molecular Weight of Polypeptide: 50.359 kD (from nucleotide sequence), 50.0 kD (experimental) [Masai07 ]

Unification Links: Protein Model Portal:A2PZP3 , UniProt:A2PZP3

Relationship Links: Entrez-Nucleotide:PART-OF:AB287332 , InterPro:IN-FAMILY:IPR015590 , InterPro:IN-FAMILY:IPR016160 , InterPro:IN-FAMILY:IPR016161 , InterPro:IN-FAMILY:IPR016162 , InterPro:IN-FAMILY:IPR016163 , Pfam:IN-FAMILY:PF00171 , Prosite:IN-FAMILY:PS00687

Gene-Reaction Schematic: ?

Credits:
Created 25-Sep-2009 by Caspi R , SRI International


Enzymatic reaction of: vanillin dehydrogenase

EC Number: 1.2.1.67

vanillin + NAD+ + H2O <=> vanillate + NADH + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for vanillin: benzaldehyde [Masai07 ] , 4-hydroxybenzaldehyde [Masai07 ] , protocatechualdehyde [Masai07 ] , 3-methoxybenzaldehyde [Masai07 ]

In Pathways: superpathway of vanillin and vanillate degradation , vanillin and vanillate degradation I


References

Masai07: Masai E, Yamamoto Y, Inoue T, Takamura K, Hara H, Kasai D, Katayama Y, Fukuda M (2007). "Characterization of ligV essential for catabolism of vanillin by Sphingomonas paucimobilis SYK-6." Biosci Biotechnol Biochem 71(10);2487-92. PMID: 17928721


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.