twitter

MetaCyc Enzyme: D-galacturonate reductase

Gene: GAR1 Accession Number: G-11972 (MetaCyc)

Species: Trichoderma reesei

Summary:
The subunit structure of this enzyme from Trichoderma reesei (previously known as Hypocrea jecorina) has not been reported. The apparent molecular mass of the polypeptide was determined by SDS-PAGE. The reason for the discrepancy with the calculated molecular mass is not known [Kuorelahti05].

Recombinant enzyme containing a 6xHisTag C-terminal fusion was expressed in a yeast expression system and purified [Kuorelahti05].

Molecular Weight of Polypeptide: 33.997 kD (from nucleotide sequence), 40.0 kD (experimental) [Kuorelahti05 ]

Unification Links: Entrez-Nucleotide:AY862503 , Protein Model Portal:Q3ZFI7 , String:51453.JGI22004 , UniProt:Q3ZFI7

Relationship Links: InterPro:IN-FAMILY:IPR001395 , InterPro:IN-FAMILY:IPR018170 , InterPro:IN-FAMILY:IPR020471 , InterPro:IN-FAMILY:IPR023210 , Panther:IN-FAMILY:PTHR11732 , Pfam:IN-FAMILY:PF00248 , Prints:IN-FAMILY:PR00069 , Prosite:IN-FAMILY:PS00062 , Prosite:IN-FAMILY:PS00063

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Credits:
Created 15-Apr-2010 by Fulcher CA , SRI International


Enzymatic reaction of: D-galacturonate reductase

Synonyms: D-galacturonic acid reductase

EC Number: 1.1.1.365

aldehydo-L-galactonate + NADP+ <=> aldehydo-D-galacturonate + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Kuorelahti05]

Alternative Substrates for aldehydo-D-galacturonate: D-glucuronate [Kuorelahti05 ] , DL-glyceraldehyde [Kuorelahti05 ]

In Pathways: superpathway of microbial D-galacturonate and D-glucuronate degradation , D-galacturonate degradation III

Summary:
The enzyme was specific for NADPH and could not use NADH. It could use D-glucuronate as substrate but with a higher Km of 11 mM. L-galactono-1,4-lactone was not a substrate for the enzyme. In the forward direction, the following compounds could not serve as substrate: D-glucose, D-fructose, α-D-xylopyranose, D-galactose, L-arabinose, or D-mannose. In the reverse direction the following compounds could not serve as substrate: L-gulonate, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol [Kuorelahti05].

Kinetic Parameters:

Substrate
Km (μM)
Citations
NADP+
1.0
[Kuorelahti05]
NADPH
30.0
[Kuorelahti05]
aldehydo-L-galactonate
4000.0
[Kuorelahti05]


References

Kuorelahti05: Kuorelahti S, Kalkkinen N, Penttila M, Londesborough J, Richard P (2005). "Identification in the mold Hypocrea jecorina of the first fungal D-galacturonic acid reductase." Biochemistry 44(33);11234-40. PMID: 16101307


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Fri May 29, 2015, biocyc14.