Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: D-galacturonate reductase

Gene: GAR1 Accession Number: G-11972 (MetaCyc)

Species: Trichoderma reesei

Summary:
The subunit structure of this enzyme from Trichoderma reesei (previously known as Hypocrea jecorina) has not been reported. The apparent molecular mass of the polypeptide was determined by SDS-PAGE. The reason for the discrepancy with the calculated molecular mass is not known [Kuorelahti05].

Recombinant enzyme containing a 6xHisTag C-terminal fusion was expressed in a yeast expression system and purified [Kuorelahti05].

Molecular Weight of Polypeptide: 33.997 kD (from nucleotide sequence), 40.0 kD (experimental) [Kuorelahti05 ]

Unification Links: Entrez-Nucleotide:AY862503 , Protein Model Portal:Q3ZFI7 , String:51453.JGI22004 , UniProt:Q3ZFI7

Relationship Links: InterPro:IN-FAMILY:IPR001395 , InterPro:IN-FAMILY:IPR018170 , InterPro:IN-FAMILY:IPR020471 , InterPro:IN-FAMILY:IPR023210 , Panther:IN-FAMILY:PTHR11732 , Pfam:IN-FAMILY:PF00248 , Prints:IN-FAMILY:PR00069 , Prosite:IN-FAMILY:PS00062 , Prosite:IN-FAMILY:PS00063

Gene-Reaction Schematic: ?

Credits:
Created 15-Apr-2010 by Fulcher CA , SRI International


Enzymatic reaction of: D-galacturonate reductase

Synonyms: D-galacturonic acid reductase

EC Number: 1.1.1.365

aldehydo-L-galactonate + NADP+ <=> aldehydo-D-galacturonate + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Kuorelahti05]

Alternative Substrates for aldehydo-D-galacturonate: D-glucuronate [Kuorelahti05 ] , DL-glyceraldehyde [Kuorelahti05 ]

In Pathways: superpathway of microbial D-galacturonate and D-glucuronate degradation , D-galacturonate degradation III

Summary:
The enzyme was specific for NADPH and could not use NADH. It could use D-glucuronate as substrate but with a higher Km of 11 mM. L-galactono-1,4-lactone was not a substrate for the enzyme. In the forward direction, the following compounds could not serve as substrate: D-glucose, D-fructose, α-D-xylopyranose, D-galactose, L-arabinose, or D-mannose. In the reverse direction the following compounds could not serve as substrate: L-gulonate, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol [Kuorelahti05].

Kinetic Parameters:

Substrate
Km (μM)
Citations
NADP+
1.0
[Kuorelahti05]
NADPH
30.0
[Kuorelahti05]
aldehydo-L-galactonate
4000.0
[Kuorelahti05]


References

Kuorelahti05: Kuorelahti S, Kalkkinen N, Penttila M, Londesborough J, Richard P (2005). "Identification in the mold Hypocrea jecorina of the first fungal D-galacturonic acid reductase." Biochemistry 44(33);11234-40. PMID: 16101307


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, biocyc11.