MetaCyc Enzyme: L,L-diaminopimelate aminotransferase

Gene: dapL Accession Numbers: G-12008 (MetaCyc), MJ1391

Species: Methanocaldococcus jannaschii

Organisms that synthesize L-lysine via the L-lysine biosynthesis VI pathway use the enzyme L,L-diaminopimelate aminotransferase to catalyze the direct conversion of (S)-2,3,4,5-tetrahydrodipicolinate to L,L-diaminopimelate. The pathway, which was originally characterized in green plants, is also used by certain bacteria, and some archaeabacteria belonging to the Methanobacteria and Methanococci classes.

The dapL gene from Methanocaldococcus jannaschii was cloned, expressed in Escherichia coli and purified. The protein was stable at 100° C for at least 30 min, and maximal activity was observed at 70° C. The enzyme was not able to use succinyl-DAP or acetyl-DAP as a substrate [Liu10c].

Molecular Weight of Polypeptide: 47.709 kD (from nucleotide sequence)

Unification Links: Entrez-gene:1452294 , Protein Model Portal:Q58786 , String:243232.MJ1391 , UniProt:Q58786

Relationship Links: InterPro:IN-FAMILY:IPR004838 , InterPro:IN-FAMILY:IPR004839 , InterPro:IN-FAMILY:IPR015421 , InterPro:IN-FAMILY:IPR015422 , InterPro:IN-FAMILY:IPR015424 , Pfam:IN-FAMILY:PF00155 , Prosite:IN-FAMILY:PS00105

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 03-May-2010 by Caspi R , SRI International

Enzymatic reaction of: L,L-diaminopimelate aminotransferase

EC Number:

L,L-diaminopimelate + 2-oxoglutarate <=> (S)-2,3,4,5-tetrahydrodipicolinate + L-glutamate + H2O + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

This reaction is reversible.

In Pathways: L-lysine biosynthesis VI

Cofactors or Prosthetic Groups: pyridoxal 5'-phosphate [Liu10c]

Kinetic Parameters:

Km (μM)

T(opt): 70 °C [Liu10c]

pH(opt): 8-9 [Liu10c]


Liu10c: Liu Y, White RH, Whitman WB (2010). "Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis." J Bacteriol. PMID: 20418392

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.