Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: carboxynorspermidine dehydrogenase

Gene: VC1624 Accession Number: G-12133 (MetaCyc)

Synonyms: CANSDH

Species: Vibrio cholerae

Summary:
The subunit structure of this enzyme has not been reported.

This enzyme participates in the alternative pathway of polyamine biosynthesis in Vibrio cholerae. In this pathway L-aspartate-semialdehyde serves as a carboxyaminopropyl group donor in a condensation reaction with propane-1,3-diamine, or putrescine. These reactions lead to the biosynthesis of norspermidine or spermidine, respectively (in [Lee09f]).

Map Position: [1,745,276 <- 1,746,520]

Molecular Weight of Polypeptide: 45.948 kD (from nucleotide sequence)

Unification Links: Entrez-gene:2613880 , Protein Model Portal:Q9KRL3 , String:243277.VC1624 , UniProt:Q9KRL3

Relationship Links: InterPro:IN-FAMILY:IPR005097 , InterPro:IN-FAMILY:IPR016040 , Pfam:IN-FAMILY:PF03435

Gene-Reaction Schematic: ?

Credits:
Created 09-Aug-2010 by Fulcher CA , SRI International


Enzymatic reaction of: carboxyspermidine dehydrogenase (carboxynorspermidine dehydrogenase)

EC Number: 1.5.1.43

carboxyspermidine + NADP+ + H2O <=> L-aspartate-semialdehyde + putrescine + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: superpathway of polyamine biosynthesis III , spermidine biosynthesis II

Summary:
Overexpression of the gene encoding the wild-type enzyme in a Vibrio cholerae mutant in which this gene was deleted increased spermidine levels relative to the wild-type strain, suggesting that when the enzyme is in excess, putrescine is increasingly utilized and carboxyspermidine is formed which is then converted to spermidine (see pathways spermidine biosynthesis II and superpathway of polyamine biosynthesis III) [Lee09f].


Enzymatic reaction of: carboxynorspermidine dehydrogenase

EC Number: 1.5.1.43

carboxynorspermidine + NADP+ + H2O <=> L-aspartate-semialdehyde + propane-1,3-diamine + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: superpathway of polyamine biosynthesis III , norspermidine biosynthesis

Summary:
Deletion of the gene encoding this enzyme in Vibrio cholerae led to the disappearance of both carboxynorspermidine and norspermidine. Complementation of the deletion mutant with an overexpressed wild-type copy of the gene encoding this enzyme restored norspermidine biosynthesis (see pathways norspermidine biosynthesis and superpathway of polyamine biosynthesis III) [Lee09f].


References

Lee09f: Lee J, Sperandio V, Frantz DE, Longgood J, Camilli A, Phillips MA, Michael AJ (2009). "An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae." J Biol Chem 284(15);9899-907. PMID: 19196710


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC14A.