|Gene:||slcC||Accession Number: G-12189 (MetaCyc)|
Species: Chromohalobacter salexigens DSM 3043
The native apparent molecular mass of this enzyme was determined by gel filtration chromatography, which suggested a homodimeric subunit structure [Denger10].
This novel enzyme catalyzes the conversion of the (S)-enantiomer of (RS)-3-sulfolactate (racemic sulfolactate) to 3-sulfopyruvate [Denger10]. SlcC is one of three sulfolactate oxidoreductases, the other two being ComC (EC 184.108.40.2062), which functions either biosynthetically (in pathway coenzyme M biosynthesis I) or degradatively (in pathway sulfolactate degradation I), and the membrane-bound SlcD (EC 1.1.99.-), identified in Roseovarius nubinhibens ISM [Denger09].
The enzyme was purified from crude extracts. Peptide-mass fingerprinting of the purified S-sulfolactate dehydrogenase confirmed its identity as the product of this gene [Denger10].
Enzyme activity was present in (RS)-3-sulfolactate-grown cells, but not in acetate-grown cells, demonstrating its inducibility [Denger10].
The subunit apparent molecular mass was determined by SDS-PAGE [Denger10].
Molecular Weight of Polypeptide: 33.135 kD (from nucleotide sequence), 34.0 kD (experimental) [Denger10 ]
Molecular Weight of Multimer: 61.0 kD (experimental) [Denger10]
Relationship Links: Entrez-Nucleotide:PART-OF:CP000285 , InterPro:IN-FAMILY:IPR006139 , InterPro:IN-FAMILY:IPR006140 , InterPro:IN-FAMILY:IPR016040 , Pfam:IN-FAMILY:PF00389 , Pfam:IN-FAMILY:PF02826 , Prosite:IN-FAMILY:PS00065 , Prosite:IN-FAMILY:PS00670 , Prosite:IN-FAMILY:PS00671
Enzymatic reaction of: S-sulfolactate dehydrogenase
Synonyms: S-sulfolactate oxidoreductase, S-sulfolactate:NAD+ oxidoreductase
EC Number: 220.127.116.110
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
This reaction is reversible. [Denger10]
In Pathways: sulfolactate degradation I
The assay for this enzyme was based on the 3-sulfopyruvate-dependent oxidation of NADH. Enzyme activity was demonstrated in both the forward and reverse directions. It was specific for NAD+ as co-substrate and NADP+ could not substitute. The Km value for (RS)-3-sulfolactate was 7 mM [Denger10].
Denger09: Denger K, Mayer J, Buhmann M, Weinitschke S, Smits TH, Cook AM (2009). "Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase." J Bacteriol 191(18);5648-56. PMID: 19581363
Denger10: Denger K, Cook AM (2010). "Racemase activity effected by two dehydrogenases in sulfolactate degradation by Chromohalobacter salexigens: purification of (S)-sulfolactate dehydrogenase." Microbiology 156(Pt 3);967-74. PMID: 20007648
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