|Gene:||aguA||Accession Number: G-29 (MetaCyc)|
Species: Pseudomonas aeruginosa
To prove the role of the aguA and aguB genes in Pseudomonas aeruginosa, the genes were knockedout in an speC mutant. Since the ornithine decarboxylase enzyme is inactive in speC background, these cells rely on agmatine deiminase and N-carbamoylputrescine amidohydrolase for putrescine biosynthesis. The mutants were putrescine auxotrophs, confirming the role of the aguA and aguB genes [Nakada03]. The enzyme was purified, and was found to be a homodimer. Agmatine was the sole substrate. Divalent ions had no effect on the enzyme, except for cationic mercury, which inhibited it [Nakada03].
Molecular Weight of Polypeptide: 41.191 kD (from nucleotide sequence), 43 kD (experimental) [Nakada03 ]
Molecular Weight of Multimer: 89 kD (experimental) [Nakada03]
Enzymatic reaction of: agmatine deiminase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
T(opt): 45 °C [Nakada03]
pH(opt): 8 [Nakada03]
Nakada01: Nakada Y, Jiang Y, Nishijyo T, Itoh Y, Lu CD (2001). "Molecular characterization and regulation of the aguBA operon, responsible for agmatine utilization in Pseudomonas aeruginosa PAO1." J Bacteriol 183(22);6517-24. PMID: 11673419
Nakada03: Nakada Y, Itoh Y (2003). "Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway." Microbiology 149(Pt 3);707-14. PMID: 12634339
Stover00: Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV (2000). "Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen." Nature 406(6799);959-64. PMID: 10984043
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