Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
twitter

MetaCyc Enzyme: UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglocosaminephosphotransferase

Gene: ALG7 Accession Number: G-5522 (MetaCyc)

Synonyms: TUR1, UDP-N-acetylglucosamine: dolichyl phosphate, N-acetylglucosamine-1-phosphate transferase, UDP-N-acetylglucosamine-1-P transferase, UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, UDP-N-acetyl-D-glucosamine:dolichyl-phosphate N-acetyl-D-glucosaminephosphotransferase, N-acetyl glucosaminyl phosphate transferase

Species: Saccharomyces cerevisiae

Summary:
The enzyme contains a 13-amino acid consensus sequence thought to be involved in dolichol recognition. This sequence is also found in the glycosyltransferase products of genes ALG1 and DPM1, and the product of gene SEC59 [Albright89].
The ALG7 gene is essential for cell growth [Kukuruzinska87]

Citations: [Burda99, Sharma82, Barnes84, Kukuruzinska87]

Gene Citations: [Barnes84]

Locations: endoplasmic reticulum

Map Position: [705,442 <- 706,788]

Unification Links: DIP:DIP-5364N , Entrez-gene:852545 , Mint:MINT-536736 , ModBase:P07286 , Protein Model Portal:P07286 , String:4932.YBR243C , Swiss-Model:P07286 , UniProt:P07286

Relationship Links: InterPro:IN-FAMILY:IPR000715 , Pfam:IN-FAMILY:PF00953

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005783 - endoplasmic reticulum [Marriott79]


Enzymatic reaction of: UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglocosaminephosphotransferase

EC Number: 2.7.8.15

a dolichyl phosphate + UDP-N-acetyl-α-D-glucosamine <=> an N-acetylglucosaminyl-diphosphodolichol + UMP

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: dolichyl-diphosphooligosaccharide biosynthesis

Summary:
Tunicamycin is a specific inhibitor of this enzyme in the pathway [Rine83].

Activators (Unknown Mechanism): Mg2+ [Sharma82]

Inhibitors (Unknown Mechanism): UDP [Sharma82] , UMP [Sharma82] , tunicamycin [Sharma82]

Kinetic Parameters:

Substrate
Km (μM)
Citations
a dolichyl phosphate
180.0
[Sharma82]
UDP-N-acetyl-α-D-glucosamine
15.0
[Sharma82]

pH(opt): 7.5 [Sharma82]


References

Albright89: Albright CF, Orlean P, Robbins PW (1989). "A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition." Proc Natl Acad Sci U S A 86(19);7366-9. PMID: 2678101

Barnes84: Barnes G, Hansen WJ, Holcomb CL, Rine J (1984). "Asparagine-linked glycosylation in Saccharomyces cerevisiae: genetic analysis of an early step." Mol Cell Biol 4(11);2381-8. PMID: 6096695

Burda99: Burda P, Aebi M (1999). "The dolichol pathway of N-linked glycosylation." Biochim Biophys Acta 1426(2);239-57. PMID: 9878760

Kukuruzinska87: Kukuruzinska MA, Robbins PW (1987). "Protein glycosylation in yeast: transcript heterogeneity of the ALG7 gene." Proc Natl Acad Sci U S A 84(8);2145-9. PMID: 3031666

Marriott79: Marriott M, Tanner W (1979). "Localization of dolichyl phosphate- and pyrophosphate-dependent glycosyl transfer reactions in Saccharomyces cerevisiae." J Bacteriol 139(2);566-72. PMID: 222737

Rine83: Rine J, Hansen W, Hardeman E, Davis RW (1983). "Targeted selection of recombinant clones through gene dosage effects." Proc Natl Acad Sci U S A 80(22);6750-4. PMID: 6316322

Sato01: Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A (2001). "Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis." Genes Cells 6(6);495-506. PMID: 11442630

Sharma82: Sharma CB, Lehle L, Tanner W (1982). "Solubilization and characterization of the initial enzymes of the dolichol pathway from yeast." Eur J Biochem 126(2);319-25. PMID: 6215245


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.