Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: uroporphyrinogen decarboxylase

Gene: hemE Accession Number: G-9033 (MetaCyc)

Species: Rhodobacter sphaeroides

Summary:
The molecular weight was determined by SDS-PAGE. The native molecular weight was 39 kD by gel filtration chromatography [Jones93a].

Map Position: [2,424,282 -> 2,425,313]

Molecular Weight of Polypeptide: 41 kD (experimental) [Jones93a ]

pI: 4.4 [Jones93a]

Unification Links: Entrez-gene:3718330 , Entrez:ABA79856

Gene-Reaction Schematic: ?


Enzymatic reaction of: uroporphyrinogen decarboxylase

EC Number: 4.1.1.37

uroporphyrinogen-III + 4 H+ <=> coproporphyrinogen III + 4 CO2

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of heme biosynthesis from glycine , superpathway of bacteriochlorophyll a biosynthesis , heme biosynthesis I (aerobic) , chlorophyllide a biosynthesis II (anaerobic)

Summary:
The enzyme was subject to substrate inhibition [Jones93a].

Inhibitors (Unknown Mechanism): porphobilinogen [Jones93a] , uroporphyrin I [Jones93a] , uroporphyrin III [Jones93a] , coproporphyrin I [Jones93a] , coproporphyrin III [Jones93a] , haematoporphyrin IX [Jones93a] , iodoacetamide [Jones93a] , N-ethylmaleimide [Jones93a] , phenylglyoxal [Jones93a] , Hg2+ [Jones93a] , Cu2+ [Jones93a]

Kinetic Parameters:

Substrate
Km (μM)
Citations
uroporphyrinogen-III
6.0
[Jones93a]

pH(opt): 6.7-6.9 [Jones93a]


References

Jones93a: Jones RM, Jordan PM (1993). "Purification and properties of the uroporphyrinogen decarboxylase from Rhodobacter sphaeroides." Biochem J 293 ( Pt 3);703-12. PMID: 8352737


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc13.