|Gene:||MJ1418||Accession Number: G-9415 (MetaCyc)|
Species: Methanocaldococcus jannaschii
The Methanocaldococcus jannaschii MJ1418 gene product was expressed in Escherichia coli and purified. The enzyme was found to catalyze the condensation of a mixture of D- and L-(S)-lactaldehyde with dihydroxyacetone phosphate (DHAP) to form four different deoxyhexose sugars, including L-fuculose 1-phosphate. The specific activity of this condensation was 570 nmol/min/mg protein.
This reaction is reversible, and the authors suggest that it may be a main source for the production of (S)-lactaldehyde, which is subsequently used to form phospholactate, a precursor of oxidized coenzyme γ-F420-2.
The enzyme was able to utilize several alternative substrates, including D-glyceraldehyde. All in all, the enzyme from Methanocaldococcus jannaschii showed similar substrate specificity to the Escherichia coli K-12 FucA[Grochowski06].
Molecular Weight of Polypeptide: 20.47 kD (from nucleotide sequence), 20 kD (experimental) [Grochowski06 ]
Molecular Weight of Multimer: 90 kD (experimental) [Grochowski06]
Enzymatic reaction of: fuculose-1-phosphate aldolase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
This reaction is reversible.
Alternative Substrates for (S)-lactaldehyde: acetaldehyde [Grochowski06 ] , methylglyoxal [Grochowski06 ] , formaldehyde [Grochowski06 ] , acrolein [Grochowski06 ] , glycolaldehyde [Grochowski06 ] , D-glyceraldehyde [Grochowski06 ]
In Pathways: lactate biosynthesis (archaea)
Grochowski06: Grochowski LL, Xu H, White RH (2006). "Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis." J Bacteriol 188(8);2836-44. PMID: 16585745
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