MetaCyc Enzyme: fuculose-1-phosphate aldolase

Gene: MJ1418 Accession Number: G-9415 (MetaCyc)

Species: Methanocaldococcus jannaschii

Subunit composition of fuculose-1-phosphate aldolase = [MJ1418]4
         fuculose-1-phosphate aldolase subunit = MJ1418

The Methanocaldococcus jannaschii MJ1418 gene product was expressed in Escherichia coli and purified. The enzyme was found to catalyze the condensation of a mixture of D- and L-(S)-lactaldehyde with glycerone phosphate (DHAP) to form four different deoxyhexose sugars, including L-fuculose 1-phosphate. The specific activity of this condensation was 570 nmol/min/mg protein.

This reaction is reversible, and the authors suggest that it may be a main source for the production of (S)-lactaldehyde, which is subsequently used to form phospholactate, a precursor of oxidized coenzyme F420-2.

The enzyme was able to utilize several alternative substrates, including D-glyceraldehyde. All in all, the enzyme from Methanocaldococcus jannaschii showed similar substrate specificity to the Escherichia coli K-12 FucA[Grochowski06].

Molecular Weight of Polypeptide: 20.47 kD (from nucleotide sequence), 20 kD (experimental) [Grochowski06 ]

Molecular Weight of Multimer: 90 kD (experimental) [Grochowski06]

Unification Links: Protein Model Portal:Q58813 , String:243232.MJ1418 , UniProt:Q58813

Relationship Links: InterPro:IN-FAMILY:IPR001303 , Pfam:IN-FAMILY:PF00596 , Smart:IN-FAMILY:SM01007

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 19-May-2006 by Caspi R , SRI International

Enzymatic reaction of: fuculose-1-phosphate aldolase

EC Number:

L-fuculose 1-phosphate <=> (S)-lactaldehyde + glycerone phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for (S)-lactaldehyde: acetaldehyde [Grochowski06 ] , methylglyoxal [Grochowski06 ] , formaldehyde [Grochowski06 ] , acrolein [Grochowski06 ] , glycolaldehyde [Grochowski06 ] , D-glyceraldehyde [Grochowski06 ]

In Pathways: lactate biosynthesis (archaea)


Grochowski06: Grochowski LL, Xu H, White RH (2006). "Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis." J Bacteriol 188(8);2836-44. PMID: 16585745

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc11.