Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: adenylylsulfate:phosphate adenylyltransferase

Gene: apt Accession Number: G-9571 (MetaCyc)

Species: Thiobacillus denitrificans

Subunit composition of adenylylsulfate:phosphate adenylyltransferase = [Apt]2
         adenylylsulfate:phosphate adenylyltransferase subunit = Apt

Summary:
adenylylsulfate:phosphate adenylyltransferase (APAT) was isolated from the chemolithoautotroph Thiobacillus denitrificans, and the apt gene encoding it was cloned [Bruser00].

The enzyme is homodimeric and exhibits a specific activity of 2100 μmol/min/mg. Catalysis occurs by a ping-pong mechanism with a covalently bound AMP as reaction intermediate. adenylylsulfate:phosphate adenylyltransferase is highly similar to galactose-1-phosphate uridylyltransferase and is also related to ATP adenylyltransferase. The active site residues of galactose-1-phosphate uridylyltransferase are conserved in all three enzymes, suggesting a histidine as the nucleotide-binding residue in all of them [Bruser00].

Molecular Weight of Polypeptide: 41.518 kD (from nucleotide sequence), 41.387 kD (experimental) [Bruser00 ]

Unification Links: Protein Model Portal:Q9LA72 , UniProt:Q9LA72

Relationship Links: Entrez-Nucleotide:PART-OF:AF148553 , InterPro:IN-FAMILY:IPR001937 , InterPro:IN-FAMILY:IPR005849 , InterPro:IN-FAMILY:IPR011146 , Panther:IN-FAMILY:PTHR11943 , Pfam:IN-FAMILY:PF01087

Gene-Reaction Schematic: ?

Credits:
Created 11-Sep-2006 by Caspi R , SRI International


Enzymatic reaction of: adenylylsulfate:phosphate adenylyltransferase

EC Number: 2.7.7.5

sulfate + ADP + H+ <=> adenosine 5'-phosphosulfate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: sulfite oxidation II

Kinetic Parameters:

Substrate
Km (μM)
Citations
phosphate
12000.0
[Bruser00]
adenosine 5'-phosphosulfate
300.0
[Bruser00]


References

Bruser00: Bruser T, Selmer T, Dahl C (2000). ""ADP sulfurylase" from Thiobacillus denitrificans is an adenylylsulfate:phosphate adenylyltransferase and belongs to a new family of nucleotidyltransferases." J Biol Chem 275(3);1691-8. PMID: 10636864


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, biocyc13.