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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: tartronate semialdehyde reductase 2

Gene: glxR Accession Numbers: G6278 (MetaCyc), b0509, ECK0502

Synonyms: glxB1, ybbQ

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of tartronate semialdehyde reductase 2 = [GlxR]4
         tartronate semialdehyde reductase 2 = GlxR

Summary:
Tartronate semialdehyde reductase 2 (GlxR) catalyzes the reversible reactions of NAD+-dependent oxidation of D-glycerate and the NADH-dependent reduction of tartronate semialdehyde [Njau00].

Expression of tartronate semialdehyde reductase is induced by anaerobic growth with allantoin as the sole source of nitrogen [Cusa99] and is induced more than 100-fold by growth on glyoxylate as the sole source of carbon [Njau00]. A glxR mutant lacks the ability to utilize glyoxylate [Cusa99].

Review: [Njau01]

Locations: cytosol

Map Position: [535,810 -> 536,688]

Molecular Weight of Polypeptide: 30.801 kD (from nucleotide sequence), 35.0 kD (experimental) [Njau00 ]

Molecular Weight of Multimer: 150.0 kD (experimental) [Njau00]

Unification Links: ASAP:ABE-0001759 , EchoBASE:EB3052 , EcoGene:EG13265 , EcoliWiki:b0509 , ModBase:P77161 , OU-Microarray:b0509 , PortEco:glxR , Pride:P77161 , Protein Model Portal:P77161 , RefSeq:NP_415042 , RegulonDB:G6278 , SMR:P77161 , String:511145.b0509 , Swiss-Model:P77161 , UniProt:P77161

Relationship Links: InterPro:IN-FAMILY:IPR002204 , InterPro:IN-FAMILY:IPR006115 , InterPro:IN-FAMILY:IPR006398 , InterPro:IN-FAMILY:IPR008927 , InterPro:IN-FAMILY:IPR013328 , InterPro:IN-FAMILY:IPR015815 , InterPro:IN-FAMILY:IPR016040 , Panther:IN-FAMILY:PTHR22981 , Pfam:IN-FAMILY:PF03446 , Prosite:IN-FAMILY:PS00895

Gene-Reaction Schematic: ?

Instance reaction of [D-glycerate + NAD(P)+ ← tartronate semialdehyde + NAD(P)H + H+] (1.1.1.60):
i1: D-glycerate + NAD+ ↔ tartronate semialdehyde + NADH + H+ (1.1.1.-)

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0009436 - glyoxylate catabolic process Inferred from experiment [Cusa99]
GO:0009442 - allantoin assimilation pathway Inferred from experiment [Cusa99]
GO:0006098 - pentose-phosphate shunt Inferred by computational analysis [GOA01]
GO:0006573 - valine metabolic process Inferred by computational analysis [GOA01]
GO:0046296 - glycolate catabolic process Inferred by computational analysis [UniProtGOA12]
GO:0046487 - glyoxylate metabolic process Inferred by computational analysis [GOA01]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01]
Molecular Function: GO:0008679 - 2-hydroxy-3-oxopropionate reductase activity Inferred from experiment Inferred by computational analysis [GOA01a, GOA01, Njau00]
GO:0004616 - phosphogluconate dehydrogenase (decarboxylating) activity Inferred by computational analysis [GOA01]
GO:0008442 - 3-hydroxyisobutyrate dehydrogenase activity Inferred by computational analysis [GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred by computational analysis [GOA01]
GO:0050662 - coenzyme binding Inferred by computational analysis [GOA01]
GO:0051287 - NAD binding Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds
metabolism central intermediary metabolism glyoxylate degradation

Credits:
Created in EcoCyc 22-Aug-2007 by Keseler I , SRI International
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: tartronate semialdehyde reductase

Synonyms: TSA reductase, 2-hydroxy-3-oxopropionate reductase, (R)-glycerate:NAD(P)+ oxidoreductase

EC Number: 1.1.1.-

D-glycerate + NAD+ <=> tartronate semialdehyde + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Njau00]

In Pathways: superpathway of glycol metabolism and degradation , glycolate and glyoxylate degradation I

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Summary:
The optimal pH for the NADH-dependent reduction of tartronate semialdehyde is 6-6.5, while the optimal pH for the NAD-dependent oxidation of D-glycerate is 9.5 [Njau00].

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-glycerate
280.0, 278.0
[Njau00, BRENDA14]
NAD+
28.0
[Njau00]


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 4 -> 18
[UniProt10]
UniProt: NAD; Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 94
[UniProt10]
UniProt: NAD; Non-Experimental Qualifier: by similarity;
Active-Site 169
[UniProt10]
UniProt: Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 237
[UniProt10]
UniProt: NAD; Non-Experimental Qualifier: by similarity;

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Cusa99: Cusa E, Obradors N, Baldoma L, Badia J, Aguilar J (1999). "Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli." J Bacteriol 1999;181(24);7479-84. PMID: 10601204

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

Njau00: Njau RK, Herndon CA, Hawes JW (2000). "Novel beta -hydroxyacid dehydrogenases in Escherichia coli and Haemophilus influenzae." J Biol Chem 275(49);38780-6. PMID: 10978349

Njau01: Njau RK, Herndon CA, Hawes JW (2001). "New developments in our understanding of the beta-hydroxyacid dehydrogenases." Chem Biol Interact 130-132(1-3);785-91. PMID: 11306094

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC13A.