|Gene:||ubiF||Accession Numbers: G6365 (MetaCyc), b0662, ECK0654|
Species: Escherichia coli K-12 substr. MG1655
2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase catalyzes the third monooxygenase reaction in the ubiquinone biosynthesis pathway. The enzyme has not been characterized biochemically.
A ubiF mutant accumulates 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone (MMQ-8) [Young71, Kwon00c] and has only a slight growth defect [Wallace77a]. MMQ-8 itself can function fairly efficiently (~50%) as an electron carrier in the NADH, D-lactate, and glycerol-3-phosphate dehydrogenase systems, but only poorly for the oxidation of succinate [Wallace77a]. ubiF mutants also have reduced capacity for uptake of aminoglycoside antibiotics [Muir81] and are resistant to phleomycin, bleomycin, and heat inactivation [Collis89].
Mutants in genes ubiF, ubiH, ubiX, ubiG, and ubiE from the Keio K-12 mutant collection showed elevated resistance to the broad-spectrum antibiotic D-cycloserine (DCS) when grown in complex media, suggesting that they may be involved in DCS sensitivity [Baisa13].
However, genes ubiF and sucB have been shown to be involved in the survival of small populations of slowly, or non-growing bacteria that are phenotypically resistant to antibiotics (referred to as persisters). Mutants in ubiF and sucB from the E. coli Keio deletion mutant library had higher susceptibility to various antibiotics (ampicillin, norfloxacin, gentamicin, tetracycline and trimethoprim) and several different stress conditions, compared with the parent strain. The potential of these genes as new drug and vaccine targets was noted [Ma10].
|Map Position: [694,324 -> 695,499]|
Molecular Weight of Polypeptide: 42.954 kD (from nucleotide sequence)
pI: 6.64 [Kwon00c]
Unification Links: ASAP:ABE-0002265 , DIP:DIP-11069N , EchoBASE:EB3422 , EcoGene:EG13658 , EcoliWiki:b0662 , ModBase:P75728 , OU-Microarray:b0662 , PortEco:ubiF , PR:PRO_000024158 , Pride:P75728 , Protein Model Portal:P75728 , RefSeq:NP_415195 , RegulonDB:G6365 , SMR:P75728 , String:511145.b0662 , UniProt:P75728
Relationship Links: InterPro:IN-FAMILY:IPR002938 , InterPro:IN-FAMILY:IPR003042 , InterPro:IN-FAMILY:IPR010971 , InterPro:IN-FAMILY:IPR018168 , Pfam:IN-FAMILY:PF01494 , Prints:IN-FAMILY:PR00420 , Prosite:IN-FAMILY:PS01304
|Biological Process:||GO:0006744 - ubiquinone biosynthetic process
[UniProtGOA12, UniProtGOA11a, GOA01a, Young71]
GO:0008152 - metabolic process [GOA01a]
GO:0044550 - secondary metabolite biosynthetic process [Gaudet10]
GO:0055114 - oxidation-reduction process [UniProtGOA11a, GOA01a]
|Molecular Function:||GO:0005515 - protein binding
GO:0008682 - 2-octoprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase activity [Kwon00c]
GO:0004497 - monooxygenase activity [UniProtGOA11a, Gaudet10]
GO:0016491 - oxidoreductase activity [UniProtGOA11a, GOA01a]
GO:0016705 - oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen [Kwon00c]
GO:0016709 - oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen [GOA01a]
GO:0050660 - flavin adenine dinucleotide binding [GOA01a]
|Cellular Component:||GO:0005829 - cytosol [DiazMejia09]|
|MultiFun Terms:||metabolism → biosynthesis of building blocks → cofactors, small molecule carriers → menaquinone, ubiquinone|
Enzymatic reaction of: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase
EC Number: 1.14.-.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
6/20/2000 (pkarp) Merged genes G211/ubiF and G6365/b0662
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Collis89: Collis CM, Grigg GW (1989). "An Escherichia coli mutant resistant to phleomycin, bleomycin, and heat inactivation is defective in ubiquinone synthesis." J Bacteriol 1989;171(9);4792-8. PMID: 2475481
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Ma10: Ma C, Sim S, Shi W, Du L, Xing D, Zhang Y (2010). "Energy production genes sucB and ubiF are involved in persister survival and tolerance to multiple antibiotics and stresses in Escherichia coli." FEMS Microbiol Lett 303(1);33-40. PMID: 20041955
Muir81: Muir ME, Hanwell DR, Wallace BJ (1981). "Characterization of a respiratory mutant of Escherichia coli with reduced uptake of aminoglycoside antibiotics." Biochim Biophys Acta 638(2);234-41. PMID: 7032590
Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554
Young71: Young IG, McCann LM, Stroobant P, Gibson F (1971). "Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone." J Bacteriol 1971;105(3);769-78. PMID: 4323297
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