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MetaCyc Enzyme: inositol polyphosphate 5-phosphatase OCRL1

Gene: OCRL Accession Number: HS04546 (MetaCyc)

Synonyms: INPP5F, OCRL1, LOCR, Lowe oculocerebrorenal syndrome protein, oculocerebrorenal syndrome of Lowe

Species: Homo sapiens

Summary:
General Background

The signalling activities of both D-myo-inositol (1,4,5)-trisphosphate and D-myo-inositol (1,3,4,5)-tetrakisphosphate are terminated by hydrolysis of their 5-phosphate groups. The inositol polyphosphate 5-phosphatases have been subdivided into four subgroups based on their size and substrate specificity [Jefferson97, Erneux98].

Type I enzymes hydrolyze only the water soluble D-myo-inositol (1,4,5)-trisphosphate and D-myo-inositol (1,3,4,5)-tetrakisphosphate [Verjans94, Laxminarayan94].

Type II enzymes also accept the insoluble phospholipids 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate and a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate as substrates [Jefferson95, Jackson95].

Type III enzymes hydrolyze only phosphoinositides and inositol polyphosphates that have a 3-position phosphate group, such as 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate [Jackson95].

Type IV enzymes hydrolyze 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate, 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate, and a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate D-myo-inositol (1,3,4,5)-tetrakisphosphate [Jefferson97]. They do not accept water soluble cyclitols [Kisseleva00].

About This Protein

Mutations linked to the disease oculocerebrorenal syndrome of Lowe (a rare X-linked disorder characterized by mental retardation, congenital cataracts, and Fanconi syndrome of the proximal renal tubules) led to the identification of the OCRL gene on the chromosomal region Xq25-26 [Nussbaum97, Leahey93]. The encoded protein, which is highly homologous to INPP5B [Attree92], was expressed in baculovirus-infected Sf9 insect cells and found to catalyze the reactions of a type II phospatidylinositol polyphosphate 5-phosphatase [Zhang95a].

The OCRL protein has been found in the Golgi complex in fibroblasts [OlivosGlander95] and in lysosomes in kidney proximal tubule cell lines of a patient with Lowe syndrome [Zhang98e].

Locations: Golgi vesicle

Map Position: [125,520,032 -> 125,572,317]

Molecular Weight of Polypeptide: 104.2 kD (from nucleotide sequence)

Unification Links: Entrez-Nucleotide:M88162 , Entrez-Nucleotide:S62085 , Entrez-Nucleotide:U57627 , Mint:MINT-5004450 , PhosphoSite:Q01968 , Pride:Q01968 , Protein Model Portal:Q01968 , SMR:Q01968 , String:Q01968 , UniProt:Q01968

Relationship Links: InterPro:IN-FAMILY:IPR000198 , InterPro:IN-FAMILY:IPR000300 , InterPro:IN-FAMILY:IPR005135 , InterPro:IN-FAMILY:IPR008936 , PDB:Structure:2KIE , PDB:Structure:2QV2 , PDB:Structure:3QBT , PDB:Structure:3QIS , Pfam:IN-FAMILY:PF00620 , Pfam:IN-FAMILY:PF03372 , Prosite:IN-FAMILY:PS50003 , Prosite:IN-FAMILY:PS50238 , Smart:IN-FAMILY:SM00128 , Smart:IN-FAMILY:SM00324

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005798 - Golgi-associated vesicle

Credits:
Created 19-Oct-2009 by Caspi R , SRI International


Enzymatic reaction of: phosphatidylinositol-4,5-bisphosphate 5-phosphatase (inositol polyphosphate 5-phosphatase OCRL1)

EC Number: 3.1.3.36

a 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O <=> a 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: 3-phosphoinositide degradation


Enzymatic reaction of: D-myo-inositol (1,4,5)-trisphosphate 5-phosphatase (inositol polyphosphate 5-phosphatase OCRL1)

EC Number: 3.1.3.56

D-myo-inositol (1,4,5)-trisphosphate + H2O <=> D-myo-inositol (1,4)-bisphosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism , D-myo-inositol (1,4,5)-trisphosphate degradation


Enzymatic reaction of: phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase (inositol polyphosphate 5-phosphatase OCRL1)

EC Number: 3.1.3.86

1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O <=> a 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: 3-phosphoinositide degradation


Enzymatic reaction of: inositol-1,3,4,5-tetrakisphosphate 5-phosphatase (inositol polyphosphate 5-phosphatase OCRL1)

EC Number: 3.1.3.56

D-myo-inositol (1,3,4,5)-tetrakisphosphate + H2O <=> D-myo-inositol (1,3,4)-trisphosphate + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism , D-myo-inositol (1,3,4)-trisphosphate biosynthesis , 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)

Exons/Introns:


References

Attree92: Attree O, Olivos IM, Okabe I, Bailey LC, Nelson DL, Lewis RA, McInnes RR, Nussbaum RL (1992). "The Lowe's oculocerebrorenal syndrome gene encodes a protein highly homologous to inositol polyphosphate-5-phosphatase." Nature 358(6383);239-42. PMID: 1321346

Erneux98: Erneux C, Govaerts C, Communi D, Pesesse X (1998). "The diversity and possible functions of the inositol polyphosphate 5-phosphatases." Biochim Biophys Acta 1436(1-2);185-99. PMID: 9838104

Jackson95: Jackson SP, Schoenwaelder SM, Matzaris M, Brown S, Mitchell CA (1995). "Phosphatidylinositol 3,4,5-trisphosphate is a substrate for the 75 kDa inositol polyphosphate 5-phosphatase and a novel 5-phosphatase which forms a complex with the p85/p110 form of phosphoinositide 3-kinase." EMBO J 14(18);4490-500. PMID: 7556092

Jefferson95: Jefferson AB, Majerus PW (1995). "Properties of type II inositol polyphosphate 5-phosphatase." J Biol Chem 270(16);9370-7. PMID: 7721860

Jefferson97: Jefferson AB, Auethavekiat V, Pot DA, Williams LT, Majerus PW (1997). "Signaling inositol polyphosphate-5-phosphatase. Characterization of activity and effect of GRB2 association." J Biol Chem 272(9);5983-8. PMID: 9038219

Kisseleva00: Kisseleva MV, Wilson MP, Majerus PW (2000). "The isolation and characterization of a cDNA encoding phospholipid-specific inositol polyphosphate 5-phosphatase." J Biol Chem 275(26);20110-6. PMID: 10764818

Laxminarayan94: Laxminarayan KM, Chan BK, Tetaz T, Bird PI, Mitchell CA (1994). "Characterization of a cDNA encoding the 43-kDa membrane-associated inositol-polyphosphate 5-phosphatase." J Biol Chem 269(25);17305-10. PMID: 8006039

Leahey93: Leahey AM, Charnas LR, Nussbaum RL (1993). "Nonsense mutations in the OCRL-1 gene in patients with the oculocerebrorenal syndrome of Lowe." Hum Mol Genet 2(4);461-3. PMID: 8504307

Lecompte08: Lecompte O, Poch O, Laporte J (2008). "PtdIns5P regulation through evolution: roles in membrane trafficking?." Trends Biochem Sci 33(10);453-60. PMID: 18774718

Nussbaum97: Nussbaum RL, Orrison BM, Janne PA, Charnas L, Chinault AC (1997). "Physical mapping and genomic structure of the Lowe syndrome gene OCRL1." Hum Genet 99(2);145-50. PMID: 9048911

OlivosGlander95: Olivos-Glander IM, Janne PA, Nussbaum RL (1995). "The oculocerebrorenal syndrome gene product is a 105-kD protein localized to the Golgi complex." Am J Hum Genet 57(4);817-23. PMID: 7573041

Rohde02: Rohde G, Wenzel D, Haucke V (2002). "A phosphatidylinositol (4,5)-bisphosphate binding site within mu2-adaptin regulates clathrin-mediated endocytosis." J Cell Biol 158(2);209-14. PMID: 12119359

Verjans94: Verjans B, De Smedt F, Lecocq R, Vanweyenberg V, Moreau C, Erneux C (1994). "Cloning and expression in Escherichia coli of a dog thyroid cDNA encoding a novel inositol 1,4,5-trisphosphate 5-phosphatase." Biochem J 300 ( Pt 1);85-90. PMID: 8198557

Zhang07a: Zhang Y, Zolov SN, Chow CY, Slutsky SG, Richardson SC, Piper RC, Yang B, Nau JJ, Westrick RJ, Morrison SJ, Meisler MH, Weisman LS (2007). "Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice." Proc Natl Acad Sci U S A 104(44);17518-23. PMID: 17956977

Zhang95a: Zhang X, Jefferson AB, Auethavekiat V, Majerus PW (1995). "The protein deficient in Lowe syndrome is a phosphatidylinositol-4,5-bisphosphate 5-phosphatase." Proc Natl Acad Sci U S A 92(11);4853-6. PMID: 7761412

Zhang98e: Zhang X, Hartz PA, Philip E, Racusen LC, Majerus PW (1998). "Cell lines from kidney proximal tubules of a patient with Lowe syndrome lack OCRL inositol polyphosphate 5-phosphatase and accumulate phosphatidylinositol 4,5-bisphosphate." J Biol Chem 273(3);1574-82. PMID: 9430698


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, BIOCYC13A.