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discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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BioCyc websites down
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MetaCyc Enzyme: antigen 85C

Gene: fbpC Accession Numbers: RV0129C (MetaCyc), Rv0129c

Synonyms: mycolyl transferase 85C, fibronectin-bindng protein C, secreted antigen 85-C fbpC

Species: Mycobacterium tuberculosis H37Rv

Summary:
Mycobacterium tuberculosis H37Rv expresses three closely related mycolyl tranferases, also known as the antigen 85 (Ag85) complex. All three contribute to cell wall synthesis by catalyzing transfer of mycolic acid from one trehalose 6-monomycolate (TMM) to another, forming trehalose 6,6'-dimycolate [Belisle97] as well as the transfer of mycolate from TMM to arabinogalactan, forming the cell wall arabinogalactan-mycolate polymer [Takayama05].

The three antigen 85 variants are expressed at a steady-state ratio of 3:2:1 (ag85B: ag85A:ag85C), making antigen 85B the most abundant protein exported by M. tuberculosis (although it is the least active of the three) [Anderson01].

antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. Crystal structure of antigen 85C revealed a cannonical α/β hydrolase fold; a catalytic triad of Ser,Glu and His; a probable trehalose-mono-mycolate binding site; and a probable site for interaction with human fibronectin [Ronning00].

Gene Citations: [Wiker90]

Map Position: [156,576 <- 157,598] (3.55 centisomes)

Molecular Weight of Polypeptide: 36.771 kD (from nucleotide sequence)

Unification Links: Entrez-gene:886885 , Pride:P0A4V4 , Protein Model Portal:P0A4V4 , SMR:P0A4V4 , String:83332.Rv0129c , UniProt:P0A4V4

Relationship Links: InterPro:IN-FAMILY:IPR000801 , PDB:Structure:1DQY , PDB:Structure:1DQZ , PDB:Structure:1VA5 , PDB:Structure:3HRH , Pfam:IN-FAMILY:PF00756

Gene-Reaction Schematic: ?

Credits:
Revised 15-Dec-2009 by Caspi R , SRI International


Enzymatic reaction of: arabinogalactan:trehalose-trans-keto-mycolate mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

a mycobacterial arabinogalactan + trehalose-trans-keto-mono-mycolate <=> arabinogalactan-trans-keto-mycolate + α,α-trehalose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from trehalose-trans-keto-mono-mycolate to cell wall an arabinogalactan to form arabinogalactan-trans-keto-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that a mutation in fbpC reduced the transfer of mycolates on an arabinogalactan [Jackson99]. Overproduction of antigen 85C in the fbpC-inactivated mutant strian of M. tuberculosis restored the cell wall-linked mycolate defect [Puech02].


Enzymatic reaction of: arabinogalactan:trehalose-cis-keto-mycolate mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

a mycobacterial arabinogalactan + trehalose-cis-keto-mono-mycolate <=> arabinogalactan-cis-keto-mycolate + α,α-trehalose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from trehalose-cis-keto-mono-mycolate to cell wall an arabinogalactan to form arabinogalactan-cis-keto-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that a mutation in fbpC reduced the transfer of mycolates on an arabinogalactan [Jackson99]. Overproduction of antigen 85C in the fbpC-inactivated mutant strian of M. tuberculosis restored the cell wall-linked mycolate defect [Puech02].


Enzymatic reaction of: arabinogalactan:trehalose-trans-methoxy-mono-mycolate mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

a mycobacterial arabinogalactan + trehalose-trans-methoxy-mono-mycolate <=> arabinogalactan-trans-methoxy-mycolate + α,α-trehalose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from trehalose-trans-methoxy-mono-mycolate to cell wall an arabinogalactan to form arabinogalactan-trans-methoxy-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that a mutation in fbpC reduced the transfer of mycolates on an arabinogalactan [Jackson99]. Overproduction of antigen 85C in the fbpC-inactivated mutant strian of M. tuberculosis restored the cell wall-linked mycolate defect [Puech02].


Enzymatic reaction of: arabinogalactan: trehalose-cis-methoxy-mycolate mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

a mycobacterial arabinogalactan + trehalose-cis-methoxy-mono-mycolate <=> arabinogalactan-cis-methoxy-mycolate + α,α-trehalose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from trehalose-cis-methoxy-mono-mycolate to cell wall an arabinogalactan to form arabinogalactan-cis-methoxy-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that a mutation in fbpC reduced the transfer of mycolates on an arabinogalactan [Jackson99]. Overproduction of antigen 85C in the fbpC-inactivated mutant strian of M. tuberculosis restored the cell wall-linked mycolate defect [Puech02].


Enzymatic reaction of: arabinogalactan: trehalose-mono-mycolate mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

a mycobacterial arabinogalactan + trehalose-mono-mycolate <=> mycolyl-arabinogalactan-peptidoglycan complex + α,α-trehalose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis , superpathway of mycolate biosynthesis , mycolyl-arabinogalactan-peptidoglycan complex biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from trehalose-mono-mycolate to cell wall an arabinogalactan to form mycolyl-arabinogalactan-peptidoglycan complex outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that a mutation in fbpC reduced the transfer of mycolates on an arabinogalactan [Jackson99]. Overproduction of antigen 85C in the fbpC-inactivated mutant strian of M. tuberculosis restored the cell wall-linked mycolate defect [Puech02].


Enzymatic reaction of: trehalose-trans-keto-mycolate:trehalose-trans-keto-mycolate 6-mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

2 trehalose-trans-keto-mono-mycolate[periplasmic space] <=> trehalose-trans-keto-di-mycolate[periplasmic space] + α,α-trehalose[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from one trehalose-trans-keto-mono-mycolate to another trehalose-trans-keto-mono-mycolate to form trehalose-trans-keto-di-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that TDM is formed by |RV0129C-MONOMER| in mycolyltransferase assay [Belisle97].


Enzymatic reaction of: trehalose-cis-keto-mono-mycolate:trehalose-cis-keto-mono-mycolate 6-mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

2 trehalose-cis-keto-mono-mycolate[periplasmic space] <=> trehalose-cis-keto-di-mycolate[periplasmic space] + α,α-trehalose[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from one trehalose-cis-keto-mono-mycolate to another trehalose-cis-keto-mono-mycolate to form trehalose-cis-keto-di-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that TDM is formed by |RV0129C-MONOMER| in mycolyltransferase assay [Belisle97].


Enzymatic reaction of: trehalose-trans-methoxy-mono-mycolate:trehalose-trans-methoxy-mono-mycolate 6-mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

2 trehalose-trans-methoxy-mono-mycolate[periplasmic space] <=> trehalose-trans-methoxy-di-mycolate[periplasmic space] + α,α-trehalose[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from one trehalose-trans-methoxy-mono-mycolate to another trehalose-trans-methoxy-mono-mycolate to form trehalose-trans-methoxy-di-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that TDM is formed by |RV0129C-MONOMER| in mycolyltransferase assay [Belisle97].


Enzymatic reaction of: trehalose-cis-methoxy-mycolate:trehalose-cis-methoxy-mycolate 6-mycolyltransferase (antigen 85C)

EC Number: 2.3.1.-

2 trehalose-cis-methoxy-mono-mycolate[periplasmic space] <=> trehalose-cis-methoxy-di-mycolate[periplasmic space] + α,α-trehalose[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from one trehalose-cis-methoxy-mono-mycolate to another trehalose-cis-methoxy-mono-mycolate to form trehalose-cis-methoxy-di-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that TDM is formed by |RV0129C-MONOMER| in mycolyltransferase assay [Belisle97].


Enzymatic reaction of: α,α-trehalose-6-mycolate:α,α-trehalose-6-mycolate 6-mycolyltransferase (antigen 85C)

EC Number: 2.3.1.122

2 trehalose-mono-mycolate <=> α, α'-trehalose 6,6'-bismycolate + α,α-trehalose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of mycolate biosynthesis , mycolate biosynthesis

Summary:
antigen 85C is an extracellular mycolyltransferase that catalyzes the transfer of mycolic acid from one trehalose-mono-mycolate to another trehalose-mono-mycolate to form trehalose-di-mycolate outside the cells. antigen 85C shares 65 to 80 percent identity with antigen 85A and antigen 85B [Takayama05]. TLC shows that TDM is formed by |RV0129C-MONOMER| in mycolyltransferase assay [Belisle97].


References

Anderson01: Anderson DH, Harth G, Horwitz MA, Eisenberg D (2001). "An interfacial mechanism and a class of inhibitors inferred from two crystal structures of the Mycobacterium tuberculosis 30 kDa major secretory protein (Antigen 85B), a mycolyl transferase." J Mol Biol 307(2);671-81. PMID: 11254389

Belisle97: Belisle JT, Vissa VD, Sievert T, Takayama K, Brennan PJ, Besra GS (1997). "Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis." Science 276(5317);1420-2. PMID: 9162010

Jackson99: Jackson M, Raynaud C, Laneelle MA, Guilhot C, Laurent-Winter C, Ensergueix D, Gicquel B, Daffe M (1999). "Inactivation of the antigen 85C gene profoundly affects the mycolate content and alters the permeability of the Mycobacterium tuberculosis cell envelope." Mol Microbiol 31(5);1573-87. PMID: 10200974

Puech02: Puech V, Guilhot C, Perez E, Tropis M, Armitige LY, Gicquel B, Daffe M (2002). "Evidence for a partial redundancy of the fibronectin-binding proteins for the transfer of mycoloyl residues onto the cell wall arabinogalactan termini of Mycobacterium tuberculosis." Mol Microbiol 44(4);1109-22. PMID: 12010501

Ronning00: Ronning DR, Klabunde T, Besra GS, Vissa VD, Belisle JT, Sacchettini JC (2000). "Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines." Nat Struct Biol 7(2);141-6. PMID: 10655617

Takayama05: Takayama K, Wang C, Besra GS (2005). "Pathway to synthesis and processing of mycolic acids in Mycobacterium tuberculosis." Clin Microbiol Rev 18(1);81-101. PMID: 15653820

Wiker90: Wiker HG, Sletten K, Nagai S, Harboe M (1990). "Evidence for three separate genes encoding the proteins of the mycobacterial antigen 85 complex." Infect Immun 58(1);272-4. PMID: 2403534


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.