MetaCyc Enzyme: agmatine deiminase

Gene: aguA Accession Number: G-29 (MetaCyc)

Species: Pseudomonas aeruginosa

Subunit composition of agmatine deiminase = [AguA]2
         agmatine deiminase subunit = AguA

To prove the role of the aguA and aguB genes in Pseudomonas aeruginosa, the genes were knockedout in an speC mutant. Since the ornithine decarboxylase enzyme is inactive in speC background, these cells rely on agmatine deiminase and N-carbamoylputrescine amidohydrolase for putrescine biosynthesis. The mutants were putrescine auxotrophs, confirming the role of the aguA and aguB genes [Nakada03]. The enzyme was purified, and was found to be a homodimer. Agmatine was the sole substrate. Divalent ions had no effect on the enzyme, except for cationic mercury, which inhibited it [Nakada03].

Gene Citations: [Stover00, Nakada01]

Molecular Weight of Polypeptide: 41.191 kD (from nucleotide sequence), 43 kD (experimental) [Nakada03 ]

Molecular Weight of Multimer: 89 kD (experimental) [Nakada03]

Unification Links: ModBase:Q9I6J9 , Protein Model Portal:Q9I6J9 , SMR:Q9I6J9 , String:208964.PA0292 , Swiss-Model:Q9I6J9 , UniProt:Q9I6J9

Relationship Links: Entrez-Nucleotide:RELATED-TO:AE004467 , InterPro:IN-FAMILY:IPR007466 , InterPro:IN-FAMILY:IPR017754 , Pfam:IN-FAMILY:PF04371

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Enzymatic reaction of: agmatine deiminase

EC Number:

agmatine + H2O <=> ammonium + N-carbamoylputrescine

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of polyamine biosynthesis II , L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) , putrescine biosynthesis II

Inhibitors (Unknown Mechanism): iodoacetamide [Nakada03] , p-chloromercuribenzoate [Nakada03] , Hg2+ [Nakada03]

Kinetic Parameters:

Km (μM)

T(opt): 45 °C [Nakada03]

pH(opt): 8 [Nakada03]


Nakada01: Nakada Y, Jiang Y, Nishijyo T, Itoh Y, Lu CD (2001). "Molecular characterization and regulation of the aguBA operon, responsible for agmatine utilization in Pseudomonas aeruginosa PAO1." J Bacteriol 183(22);6517-24. PMID: 11673419

Nakada03: Nakada Y, Itoh Y (2003). "Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway." Microbiology 149(Pt 3);707-14. PMID: 12634339

Stover00: Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV (2000). "Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen." Nature 406(6799);959-64. PMID: 10984043

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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