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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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MetaCyc Enzyme: inositol-1-phosphate synthase

Gene: AF1794 Accession Number: G-3884 (MetaCyc)

Species: Archaeoglobus fulgidus

Subunit composition of inositol-1-phosphate synthase = [AF1794]4
         inositol-1-phosphate synthase subunit = AF1794

Summary:
The Archaeoglobus fulgidus inositol-1-phosphate synthase (IPS) gene was overexpressed in Escherichia coli. This archaeal enzyme, with the highest activity at its optimum growth temperature among all IPS reported, is extremely heat stable. The most unique feature of the Archaeoglobus fulgidus enzyme is that it absolutely requires divalent metal ions for activity, while NH4+ (a critical activator for all the other characterized IPS enzymes) has no effect. These properties suggested that this archaeal IPS is a class II aldolase [Chen00c].

Molecular Weight of Polypeptide: 44 kD (experimental) [Chen00c ]

Molecular Weight of Multimer: 168 kD (experimental) [Chen00c]

Unification Links: ModBase:O28480 , Protein Model Portal:O28480 , SMR:O28480 , String:224325.AF1794 , Swiss-Model:O28480 , UniProt:O28480

Relationship Links: Entrez-Nucleotide:PART-OF:AE000979 , InterPro:IN-FAMILY:IPR002587 , InterPro:IN-FAMILY:IPR013021 , InterPro:IN-FAMILY:IPR016040 , Panther:IN-FAMILY:PTHR11510 , PDB:Structure:1U1I , PDB:Structure:3QVS , PDB:Structure:3QVT , PDB:Structure:3QVW , PDB:Structure:3QVX , PDB:Structure:3QW2 , Pfam:IN-FAMILY:PF01658 , Pfam:IN-FAMILY:PF07994

Gene-Reaction Schematic: ?

MultiFun Terms: metabolism biosynthesis of macromolecules (cellular constituents) phospholipid


Enzymatic reaction of: inositol-1-phosphate synthase

EC Number: 5.5.1.4

D-glucopyranose 6-phosphate <=> 1D-myo-inositol 3-monophosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: myo-inositol biosynthesis , di-myo-inositol phosphate biosynthesis

Activators (Allosteric): Zn2+ [Chen00c] , Mn2+ [Chen00c]

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-glucopyranose 6-phosphate
120.0
[Chen00c]


References

Chen00c: Chen L, Zhou C, Yang H, Roberts MF (2000). "Inositol-1-phosphate synthase from Archaeoglobus fulgidus is a class II aldolase." Biochemistry 39(40);12415-23. PMID: 11015222


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc11.