|Gene:||pfkA||Accession Number: G-9171 (MetaCyc)|
Synonyms: pfkC, PF1784
Species: Pyrococcus furiosus
Subunit composition of
ADP-dependent phosphofructokinase = [PfkA]4
ADP-dependent phosphofructokinase subunit = PfkA
ADP-dependent phosphofructokinase and ADP-dependent glucokinase are members of a unique family unrelated to bacterial ATP-dependent glucokinase, or eukaryotic hexokinase (reviewed in [Verhees03]).
The native molecular mass of the enzyme partially purified from cell-free extracts of Pyrocuccus furiosus was determined by gel filtration chromatography [Tuininga99].
The subunit molecular mass of the purified, recombinant protein was determined by SDS-PAGE [Tuininga99].
Gene Citations: [Kengen01]
Molecular Weight of Polypeptide: 52.337 kD (from nucleotide sequence), 52 kD (experimental) [Tuininga99 ]
Molecular Weight of Multimer: 180 kD (experimental) [Tuininga99]
Relationship Links: Entrez-Nucleotide:PART-OF:AF127909 , InterPro:IN-FAMILY:IPR007666 , InterPro:IN-FAMILY:IPR011790 , InterPro:IN-FAMILY:IPR015990 , Panther:IN-FAMILY:PTHR21208 , Pfam:IN-FAMILY:PF04587 , Prosite:IN-FAMILY:PS51255
Enzymatic reaction of: ADP-dependent phosphofructokinase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
This reaction is reversible.
In Pathways: glycolysis V (Pyrococcus)
Enzyme activity data were derived from purified, recombinant protein expressed in Escherichia coli. The Km values were determined at 50 deg. C [Tuininga99].
pH(opt): 6.5 [Tuininga99]
Kengen01: Kengen SW, Tuininga JE, Verhees CH, van der Oost J, Stams AJ, de Vos WM (2001). "ADP-dependent glucokinase and phosphofructokinase from Pyrococcus furiosus." Methods Enzymol 331;41-53. PMID: 11265478
Tuininga99: Tuininga JE, Verhees CH, van der Oost J, Kengen SW, Stams AJ, de Vos WM (1999). "Molecular and biochemical characterization of the ADP-dependent phosphofructokinase from the hyperthermophilic archaeon Pyrococcus furiosus." J Biol Chem 274(30);21023-8. PMID: 10409652
Verhees03: Verhees CH, Kengen SW, Tuininga JE, Schut GJ, Adams MW, De Vos WM, Van Der Oost J (2003). "The unique features of glycolytic pathways in Archaea." Biochem J 375(Pt 2);231-46. PMID: 12921536
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