Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: N-methylputrescine oxidase

Species: Hyoscyamus niger

Subunit composition of N-methylputrescine oxidase = [diamine oxidase]2

Molecular Weight of Polypeptide: 66 kD (experimental) [Hashimoto90 ]

Molecular Weight of Multimer: 135 kD (experimental) [Hashimoto90]

Gene-Reaction Schematic: ?


Enzymatic reaction of: N-methylputrescine oxidase

N-methylputrescine + H2O + oxygen <=> N-methylaminobutanal + ammonium + hydrogen peroxide

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for N-methylputrescine: cadaverine [Hashimoto90 ] , putrescine [Hashimoto90 ]

In Pathways: superpathway of hyoscyamine and scopolamine biosynthesis , N-methyl-Δ1-pyrrolinium cation biosynthesis

Summary:
An N-methylputrescine oxidase has been partially purified from Hyoscyamus niger. The enzyme accepts N-methylputrescine, putrescine, and cadaverine as substrates, with N-methylputrescine being the best substrate. Inhibition of the enzyme activity by copper ligands and carbonyl reagents indicated that copper and pyrroloquinoline quinine are present at the active site of the enzyme.

Cofactors or Prosthetic Groups: pyrroloquinoline quinone [Hashimoto90], Cu2+ [Hashimoto90]

Inhibitors (Unknown Mechanism): Cu2+ [Hashimoto90] , Hg2+ [Hashimoto90] , p-chloromercuribenzenesulfonic acid [Hashimoto90] , CdCl2 [Hashimoto90] , potassium cyanide [Hashimoto90] , N-methylpropylamine [Hashimoto90] , N-methylbutylamine [Hashimoto90]

Kinetic Parameters:

Substrate
Km (μM)
Citations
N-methylputrescine
330.0
[Hashimoto90]

pH(opt): 7.5-8 [Hashimoto90]


References

Hashimoto90: Hashimoto T, Mitani A, Yamada Y (1990). "Diamine Oxidase from Cultured Roots of Hyoscyamus niger: Its Function in Tropane Alkaloid Biosynthesis." Plant Physiol 93(1);216-221. PMID: 16667438


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC14B.