MetaCyc Enzyme: benzalacetone synthase
Inferred from experiment

Species: Rubus idaeus

Subunit composition of benzalacetone synthase = [BAS subunit]2

The enzyme catalyzing the decarboxylative condensation of p-coumaroyl-CoA and malonyl-CoA to form p-hydroxybenzalacetone has been partially purified from berries of raspberry ( Rubus idaeus). Assessing the substrate specificity and effects of inhibitors it was concluded that benzalacetone synthase (BST) represents a unique enzyme and differs from the closely related enzymes chalcone synthase (CHS) and stilbene synthase (STS) that also operate in this plant [BorejszaWysocki96].

note of curator:

This enzyme may or may not be identical with 4-hydroxybenzalacetone synthase. Although very similar to 4-hydroxybenzalacetone synthase with regard to catalytic properties this enzyme remains to be characterized on a molecular level to confirm identity with 4-hydroxybenzalacetone synthase (personal communication: G. Hrazdina, November 2012)

Molecular Weight of Polypeptide: 41.5 kD (experimental) [BorejszaWysocki96]

Molecular Weight of Multimer: 83 kD (experimental) [BorejszaWysocki96]

pI: 5.3 [BorejszaWysocki96]

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 09-Nov-2006 by Foerster H, TAIR
Revised 15-Sep-2010 by Pujar A, Boyce Thompson Institute
Reviewed 29-Nov-2012 by Foerster H, Boyce Thompson Institute

Enzymatic reaction of: benzalacetone synthase

Inferred from experiment

EC Number:

4-coumaryl-CoA + malonyl-CoA + H+ + H2O → 4-hydroxybenzalacetone + 2 CO2 + 2 coenzyme A

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: raspberry ketone biosynthesis

Activators (Unknown Mechanism): 2-mercaptoethanol [BorejszaWysocki96] Inhibitors (Unknown Mechanism): glutathione [BorejszaWysocki96]Kinetic Parameters:
Substrate Km (μM) Citations
malonyl-CoA 3.0 [BorejszaWysocki96]
4-coumaryl-CoA 1.0 [BorejszaWysocki96]

pH(opt): 8.0 [BorejszaWysocki96]


BorejszaWysocki96: Borejsza-Wysocki W, Hrazdina G (1996). "Aromatic Polyketide Synthases (Purification, Characterization, and Antibody Development to Benzalacetone Synthase from Raspberry Fruits)." Plant Physiol 110(3);791-799. PMID: 12226219

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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