Species: Rubus idaeus
Subunit composition of benzalacetone synthase = [BAS subunit]2
The enzyme catalyzing the decarboxylative condensation of p-coumaroyl-CoA and malonyl-CoA to form p-hydroxybenzalacetone has been partially purified from berries of raspberry ( Rubus idaeus). Assessing the substrate specificity and effects of inhibitors it was concluded that benzalacetone synthase (BST) represents a unique enzyme and differs from the closely related enzymes chalcone synthase (CHS) and stilbene synthase (STS) that also operate in this plant [BorejszaWysocki96].
note of curator:
This enzyme may or may not be identical with 4-hydroxybenzalacetone synthase. Although very similar to 4-hydroxybenzalacetone synthase with regard to catalytic properties this enzyme remains to be characterized on a molecular level to confirm identity with 4-hydroxybenzalacetone synthase (personal communication: G. Hrazdina, November 2012)
Molecular Weight of Polypeptide: 41.5 kD (experimental) [BorejszaWysocki96]
Molecular Weight of Multimer: 83 kD (experimental) [BorejszaWysocki96]
pI: 5.3 [BorejszaWysocki96]
Enzymatic reaction of: benzalacetone synthase
EC Number: 18.104.22.1684-coumaryl-CoA + malonyl-CoA + H+ + H2O → 4-hydroxybenzalacetone + 2 CO2 + 2 coenzyme A
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: raspberry ketone biosynthesisActivators (Unknown Mechanism): 2-mercaptoethanol [BorejszaWysocki96] Inhibitors (Unknown Mechanism): glutathione [BorejszaWysocki96]Kinetic Parameters:
pH(opt): 8.0 [BorejszaWysocki96]
BorejszaWysocki96: Borejsza-Wysocki W, Hrazdina G (1996). "Aromatic Polyketide Synthases (Purification, Characterization, and Antibody Development to Benzalacetone Synthase from Raspberry Fruits)." Plant Physiol 110(3);791-799. PMID: 12226219
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