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discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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MetaCyc Enzyme: benzylsuccinyl-CoA dehydrogenase

Gene: bbsG Accession Number: BBSG (MetaCyc)

Species: Thauera aromatica

Subunit composition of benzylsuccinyl-CoA dehydrogenase = [BbsG]45
         benzylsuccinyl-CoA dehydrogenase subunit = BbsG

Summary:
(R)-Benzylsuccinyl-CoA dehydrogenase belongs to the acyl-CoA dehydrogenase family.

The bbsG gene product from Thauera aromatica shows strong similarity to acyl-CoA dehydrogenases [Leuthner00]. The gene was overexpressed in Escherichia coli, and the activity of its product, (R)-benzylsuccinyl-CoA dehydrogenase, was studied [Leuthner00, Leutwein02]. Overproduced (R)-benzylsuccinyl-CoA dehydrogenase was subsequently partially purified and characterized [Leutwein02].

Substrate specificity studies of (R)-benzylsuccinyl-CoA dehydrogenase demonstrated that it has high substrate- and enantionmer-specificity [Leutwein02]. Not only is (R)-benzylsuccinyl-CoA dehydrogenase unable to oxidize the (S) isomer of benzylsuccinyl-CoA, but the enzyme was completely inhibited by its presence [Leutwein02]. A KM of 110 μM was found for (R)-benzylsuccinyl-CoA [Leutwein02]. Furthermore, it specifically forms E-phenylitconyl-CoA and not the Z isomer [Leutwein02].

(R)-Benzylsuccinyl-CoA dehydrogenase is induced by toluene- and m-xylene-grown cells of several toluene and m-xylene-degrading denitrifiers [Leutwein02].

Locations: cytosol

Molecular Weight of Polypeptide: 45.6 kD (from nucleotide sequence), 45 kD (experimental)

Molecular Weight of Multimer: 200 kD (experimental)

pI: 5.3, 5.3 [Leuthner00]

Unification Links: ModBase:Q9KJE8 , Protein Model Portal:Q9KJE8 , Swiss-Model:Q9KJE8 , UniProt:Q9KJE8

Relationship Links: InterPro:IN-FAMILY:IPR006091 , InterPro:IN-FAMILY:IPR009075 , InterPro:IN-FAMILY:IPR009100 , InterPro:IN-FAMILY:IPR013786 , Pfam:IN-FAMILY:PF00441 , Pfam:IN-FAMILY:PF02770 , Pfam:IN-FAMILY:PF02771 , Prosite:IN-FAMILY:PS00072 , Prosite:IN-FAMILY:PS00073

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005829 - cytosol


Enzymatic reaction of: benzylsuccinyl-CoA dehydrogenase

Synonyms: BbsG, BCDH, R-benzylsuccinyl-CoA dehydrogenase

EC Number: 1.3.8.3

(R)-benzylsuccinyl-CoA + 2 an oxidized electron-transfer flavoprotein <=> (E)-2-benzylidenesuccinyl-CoA + 2 a reduced electron-transfer flavoprotein

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: toluene degradation VI (anaerobic) , anaerobic aromatic compound degradation (Thauera aromatica) , toluene degradation to benzoyl-CoA (anaerobic)

Cofactors or Prosthetic Groups: FAD [Leutwein02]

Inhibitors (Unknown Mechanism): (S)-benzylsuccinyl-CoA [Leutwein02]


References

Leuthner00: Leuthner B, Heider J (2000). "Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate." J Bacteriol 2000;182(2);272-7. PMID: 10629170

Leutwein02: Leutwein C, Heider J (2002). "(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway." Arch Microbiol 178(6);517-24. PMID: 12420174


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc13.