Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

MetaCyc Enzyme: benzylsuccinyl-CoA dehydrogenase

Gene: bbsG Accession Number: BBSG (MetaCyc)

Species: Thauera aromatica

Subunit composition of benzylsuccinyl-CoA dehydrogenase = [BbsG]45
         benzylsuccinyl-CoA dehydrogenase subunit = BbsG

Summary:
(R)-Benzylsuccinyl-CoA dehydrogenase belongs to the acyl-CoA dehydrogenase family.

The bbsG gene product from Thauera aromatica shows strong similarity to acyl-CoA dehydrogenases [Leuthner00]. The gene was overexpressed in Escherichia coli, and the activity of its product, (R)-benzylsuccinyl-CoA dehydrogenase, was studied [Leuthner00, Leutwein02]. Overproduced (R)-benzylsuccinyl-CoA dehydrogenase was subsequently partially purified and characterized [Leutwein02].

Substrate specificity studies of (R)-benzylsuccinyl-CoA dehydrogenase demonstrated that it has high substrate- and enantionmer-specificity [Leutwein02]. Not only is (R)-benzylsuccinyl-CoA dehydrogenase unable to oxidize the (S) isomer of benzylsuccinyl-CoA, but the enzyme was completely inhibited by its presence [Leutwein02]. A KM of 110 μM was found for (R)-benzylsuccinyl-CoA [Leutwein02]. Furthermore, it specifically forms E-phenylitconyl-CoA and not the Z isomer [Leutwein02].

(R)-Benzylsuccinyl-CoA dehydrogenase is induced by toluene- and m-xylene-grown cells of several toluene and m-xylene-degrading denitrifiers [Leutwein02].

Locations: cytosol

Molecular Weight of Polypeptide: 45.6 kD (from nucleotide sequence), 45 kD (experimental)

Molecular Weight of Multimer: 200 kD (experimental)

pI: 5.3, 5.3 [Leuthner00]

Unification Links: ModBase:Q9KJE8 , Protein Model Portal:Q9KJE8 , Swiss-Model:Q9KJE8 , UniProt:Q9KJE8

Relationship Links: InterPro:IN-FAMILY:IPR006091 , InterPro:IN-FAMILY:IPR009075 , InterPro:IN-FAMILY:IPR009100 , InterPro:IN-FAMILY:IPR013786 , Pfam:IN-FAMILY:PF00441 , Pfam:IN-FAMILY:PF02770 , Pfam:IN-FAMILY:PF02771 , Prosite:IN-FAMILY:PS00072 , Prosite:IN-FAMILY:PS00073

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0005829 - cytosol


Enzymatic reaction of: benzylsuccinyl-CoA dehydrogenase

Synonyms: BbsG, BCDH, R-benzylsuccinyl-CoA dehydrogenase

EC Number: 1.3.8.3

(R)-benzylsuccinyl-CoA + 2 an oxidized electron-transfer flavoprotein <=> (E)-2-benzylidenesuccinyl-CoA + 2 a reduced electron-transfer flavoprotein

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: toluene degradation VI (anaerobic) , anaerobic aromatic compound degradation (Thauera aromatica) , toluene degradation to benzoyl-CoA (anaerobic)

Cofactors or Prosthetic Groups: FAD [Leutwein02]

Inhibitors (Unknown Mechanism): (S)-benzylsuccinyl-CoA [Leutwein02]


References

Leuthner00: Leuthner B, Heider J (2000). "Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate." J Bacteriol 2000;182(2);272-7. PMID: 10629170

Leutwein02: Leutwein C, Heider J (2002). "(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway." Arch Microbiol 178(6);517-24. PMID: 12420174


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, biocyc13.