|Gene:||bbsG||Accession Number: BBSG (MetaCyc)|
Species: Thauera aromatica
Subunit composition of
benzylsuccinyl-CoA dehydrogenase = [BbsG]45
benzylsuccinyl-CoA dehydrogenase subunit = BbsG
(R)-Benzylsuccinyl-CoA dehydrogenase belongs to the acyl-CoA dehydrogenase family.
The bbsG gene product from Thauera aromatica shows strong similarity to acyl-CoA dehydrogenases [Leuthner00]. The gene was overexpressed in Escherichia coli, and the activity of its product, (R)-benzylsuccinyl-CoA dehydrogenase, was studied [Leuthner00, Leutwein02]. Overproduced (R)-benzylsuccinyl-CoA dehydrogenase was subsequently partially purified and characterized [Leutwein02].
Substrate specificity studies of (R)-benzylsuccinyl-CoA dehydrogenase demonstrated that it has high substrate- and enantionmer-specificity [Leutwein02]. Not only is (R)-benzylsuccinyl-CoA dehydrogenase unable to oxidize the (S) isomer of benzylsuccinyl-CoA, but the enzyme was completely inhibited by its presence [Leutwein02]. A KM of 110 μM was found for (R)-benzylsuccinyl-CoA [Leutwein02]. Furthermore, it specifically forms E-phenylitconyl-CoA and not the Z isomer [Leutwein02].
(R)-Benzylsuccinyl-CoA dehydrogenase is induced by toluene- and m-xylene-grown cells of several toluene and m-xylene-degrading denitrifiers [Leutwein02].
Molecular Weight of Polypeptide: 45.6 kD (from nucleotide sequence), 45 kD (experimental)
Molecular Weight of Multimer: 200 kD (experimental)
pI: 5.3, 5.3 [Leuthner00]
Relationship Links: InterPro:IN-FAMILY:IPR006091 , InterPro:IN-FAMILY:IPR009075 , InterPro:IN-FAMILY:IPR009100 , InterPro:IN-FAMILY:IPR013786 , Pfam:IN-FAMILY:PF00441 , Pfam:IN-FAMILY:PF02770 , Pfam:IN-FAMILY:PF02771 , Prosite:IN-FAMILY:PS00072 , Prosite:IN-FAMILY:PS00073
|Cellular Component:||GO:0005829 - cytosol|
Enzymatic reaction of: benzylsuccinyl-CoA dehydrogenase
Synonyms: BbsG, BCDH, R-benzylsuccinyl-CoA dehydrogenase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
Leuthner00: Leuthner B, Heider J (2000). "Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate." J Bacteriol 2000;182(2);272-7. PMID: 10629170
Leutwein02: Leutwein C, Heider J (2002). "(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway." Arch Microbiol 178(6);517-24. PMID: 12420174
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